Results 131 to 140 of about 42,496 (156)

Novel allogeneic CAR T-cell platform involving microhomology-mediated end joining repair and low off-targeting potential. [PDF]

Mol Ther Nucleic Acids
Hundal T, Luo Y, Qie Y, Gadd ME, Brim AD, Vazquez-Rosario I, Guo S, Kharfan-Dabaja MA, Qin H.   +8 more
europepmc   +1 more source

CRISPR-Cas12a enables precise microhomology-mediated DNA replacement

The development of more efficient and precise methods to engineer the cellular genome is an essential aim in synthetic biology, life sciences and medicine.
GRAZIOLI, SIMONA
core  

DNA large fragment deleting by compact, sequence-motif-free and specific TaqTth-hpRNA assisted with the microhomology-mediated end joining pathway. [PDF]

Nucleic Acids Res
Liu Y, Weng Z, Zhai Z, Xu Z, Liu X, Qiang H, Tian K, Rao B, Zhou G, Guo Y, Xu S.   +10 more
europepmc   +1 more source

Particulate hexavalent chromium inhibits global transcription of genes in DNA repair pathways, particularly targeting homologous recombination repair, base excision repair, mismatch repair and microhomology-mediated end-joining. [PDF]

J Hazard Mater
Meaza I, Cahill CR, Speer RM, Kouokam JC, Wise JP.   +4 more
europepmc   +1 more source

Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks. [PDF]

BMC Biol
Yuan B, Bi C, Tian Y, Wang J, Jin Y, Alsayegh K, Tehseen M, Yi G, Zhou X, Shao Y, Romero FV, Fischle W, Izpisua Belmonte JC, Hamdan S, Huang Y, Li M.   +15 more
europepmc   +1 more source

Mitochondrial genome microhomology-mediated editing by donor DNA delivery into mitochondria in human cells. [PDF]

Mol Ther Nucleic Acids
Maximov VV, Shebanov N, Nikitchina N, Rapoport R, Maor Y, Tarassov I, Pines O, Entelis N.   +7 more
europepmc   +1 more source

Non-canonical double-stranded DNA break repair in Pseudomonas aeruginosa includes LigD-independent non-homologous end joining. [PDF]

Nucleic Acids Res
Hare PJ, González JR, Truong A, Mok WWK.   +3 more
europepmc   +1 more source

In vivo gene correction with targeted sequence substitution through microhomology-mediated end joining

Biochemical and Biophysical Research Communications, 2018
Genome editing technology using programmable nucleases has rapidly evolved in recent years. The primary mechanism to achieve precise integration of a transgene is mainly based on homology-directed repair (HDR). However, an HDR-based genome-editing approach is less efficient than non-homologous end-joining (NHEJ).
Suresh Ramakrishna, Sunghoon Kim, Junwon Lee   +2 more
exaly   +6 more sources

Homology and enzymatic requirements of microhomology-dependent alternative end joining

Cell Death and Disease, 2015
Nonhomologous DNA end joining (NHEJ) is one of the major double-strand break (DSB) repair pathways in higher eukaryotes. Recently, it has been shown that alternative NHEJ (A-NHEJ) occurs in the absence of classical NHEJ and is implicated in chromosomal ...
Sathees C Raghavan
exaly   +2 more sources

Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway

Structure, 2015
DNA polymerase theta (Polθ) has been identified as a crucial alternative non-homologous end-joining factor in mammalian cells. Polθ is upregulated in a range of cancer cell types defective in homologous recombination, and knockdown has been shown to ...
Joseph A Newman, Christopher D O Cooper, Hazel Aitkenhead   +2 more
exaly   +3 more sources