Results 111 to 120 of about 42,496 (156)

Essential Roles for Polymerase θ-Mediated End Joining in the Repair of Chromosome Breaks

, 2016
DNA polymerase theta (Pol θ)-mediated end joining (TMEJ) has been implicated in the repair of chromosome breaks, but its cellular mechanism and role relative to canonical repair pathways are poorly understood.
Ramsden, Dale A., Wyatt, David W., Feng, Wanjuan, Conlin, Michael P., Yousefzadeh, Matthew J., Mieczkowski, Piotr, Roberts, Steven A., Gupta, Gaorav P., Wood, Richard D.   +8 more
core   +1 more source

Pol θ-Mediated End-Joining Uses Microhomologies Containing Mismatches

DNA polymerase theta (Pol θ) initiates repair of DNA double-strand breaks by pairing single strands at short microhomologies . It is important to understand microhomology selection, as some cancer cells rely on Pol θ for survival.
Dang, Ngoc K, Liu, Bin, Xu, Han, Carvajal-Maldonado, Denisse, Li, Yuzhen, Returan, Mark, Wood, Richard D, He, Wei, Guerin, Adele T   +8 more
core   +1 more source

Application of an F0-based genetic assay in adult zebrafish to identify modifier genes of an inherited cardiomyopathy

Disease Models & Mechanisms, 2023
Yonghe Ding, Mingmin Wang, Haisong Bu, Jiarong Li, Xueying Lin, Xiaolei Xu   +5 more
doaj   +1 more source

RNA-mediated double-strand break repair by end-joining mechanisms

Nature Communications
Double-strand breaks (DSBs) in DNA are challenging to repair. Cells employ at least three DSB-repair mechanisms, with a preference for non-homologous end joining (NHEJ) over homologous recombination (HR) and microhomology-mediated end joining (MMEJ ...
Youngkyu Jeon, Yilin Lu, Margherita Maria Ferrari, Tejasvi Channagiri, Penghao Xu, Chance Meers, Yiqi Zhang, Sathya Balachander, Vivian S. Park, Stefania Marsili, Zachary F. Pursell, Nataša Jonoska, Francesca Storici   +12 more
doaj   +1 more source

DNA end configurations dictate synaptic complex formation during NHEJ-mediated end bridging

Nature Communications
Synapsis, the physical bridging of two broken DNA ends, is a critical step in non-homologous DNA end joining (NHEJ), the primary pathway for repairing DNA double-strand breaks (DSBs) in vertebrates.
Chenyang Zhang, Weiwei Jin, Yuhao Jiang, Weilin Li, Rongguo Fu, Keyin Qi, Wantao Li, Chao Zhao, Di Liu, Bailin Zhao   +9 more
doaj   +1 more source

Toward Precision Molecular Surgery: Robust, Selective Induction of Microhomology-mediated End Joining in vivo

, 2018
One key problem in precision genome editing is the resultant unpredictable plurality of sequence outcomes at the site of targeted DNA double-strand breaks (DSBs).
Karl J. Clark, Kyle J. Schaefbauer, Alexey V. Dvornikov, Alvin C. Ma, Hirotaka Ata, Carla M. Mann, Stephen C. Ekker, Drena Dobbs, Thomas L. Ekstrom, Gabriel Martínez-Gálvez   +9 more
core   +1 more source

The enterohemorrhagic Escherichia coli insertion sequence-excision enhancer protein is a DNA polymerase with microhomology-mediated end-joining activity. [PDF]

Nucleic Acids Res, 2023
Calvo PA, Mateo-Cáceres V, Díaz-Arco S, Redrejo-Rodríguez M, de Vega M.   +4 more
europepmc   +1 more source

The E3 ubiquitin ligase DTX3L and the deubiquitinase USP28 fine-tune DNA repair through mutual regulation of their protein levels

iScience
Summary: The DNA damage response (DDR) relies on a complex protein network to maintain genomic integrity, yet the interplay between post-translational modifiers remains poorly understood.
Daniela Mennerich, Yashwanth Ashok, Carlos Vela-Rodríguez, Heli I. Hentilä, Melanie Rall-Scharpf, Lisa Wiesmüller, Renata Prunskaite-Hyyryläinen, Lari Lehtiö, Thomas Kietzmann   +8 more
doaj   +1 more source

Recent advances in CRISPR-Cas9-based genome insertion technologies

Molecular Therapy: Nucleic Acids
Programmable genome insertion (or knock-in) is vital for both fundamental and translational research. The continuously expanding number of CRISPR-based genome insertion strategies demonstrates the ongoing development in this field.
Xinwen Chen, Jingjing Du, Shaowei Yun, Chaoyou Xue, Yao Yao, Shuquan Rao   +5 more
doaj   +1 more source

PARP1 and PARP2 are dispensable for DNA repair by microhomology-mediated end-joining during mitosis

ABSTRACT Poly ADP-ribose polymerase (PARP) inhibitors are standard of care treatment for cancers with homologous-recombination deficiencies. Yet, as tumors develop resistance, complementary strategies are emerging, including targeting microhomology-mediated end joining (MMEJ).
Raquel Ortega, Erin Taylor, Sophie M. Whitehead, Thomas Danhorn, Benjamin G. Bitler, Nausica Arnoult   +5 more
openaire   +2 more sources