Results 81 to 90 of about 42,496 (156)

Progress and Challenges in Faba Bean Genetic Engineering

Legume Science, Volume 8, Issue 2, June 2026.
ABSTRACT Legumes are essential for global food security, serving as a vital source of plant‐based protein and playing a significant role in sustainable agricultural practices. Among legumes, faba bean (Vicia faba L.) is noteworthy due to its high‐protein content, broad climate adaptation, and suitability for cultivation in temperate regions.
Simone A. Gierlich, Thilani B. Jayakody, Sruthy Maria Augustine, Huijun Liu, Marko Jääskeläinen, Triin Vaisalu, Rod. J. Snowdon, Stig U. Andersen   +7 more
wiley   +1 more source

Multiple DSB Resection Activities Redundantly Promote Alternative End Joining-Mediated Class Switch Recombination

Frontiers in Cell and Developmental Biology, 2021
Alternative end joining (A-EJ) catalyzes substantial level of antibody class switch recombination (CSR) in B cells deficient for classical non-homologous end joining, featuring increased switch (S) region DSB resection and junctional microhomology (MH ...
Xikui Sun, Xikui Sun, Jingning Bai, Jingning Bai, Jiejie Xu, Jiejie Xu, Xiaoli Xi, Mingyu Gu, Mingyu Gu, Chengming Zhu, Hongman Xue, Chun Chen, Junchao Dong, Junchao Dong, Junchao Dong   +14 more
doaj   +1 more source

Enhancing CRISPR/Cas‐Mediated Gene Knockout With Short Non‐Homologous Oligonucleotides

Plant Biotechnology Journal, Volume 24, Issue 6, Page 3742-3753, June 2026.
ABSTRACT Chlamydomonas reinhardtii is a model green microalga that has great industrial potential as a sustainable bio‐factory for recombinant protein and high‐value chemical production. Efficient genome editing tools are required to redesign this organism for synthetic biology applications. CRISPR‐Cas editing technologies have already been adapted for
Yen Peng Chew, Aron Ferenczi, Marie Dannay, Cristina Ponce‐Lilly, Adam Kovac, Dávid Tóth, Szilvia Z. Tóth, Attila Molnar   +7 more
wiley   +1 more source

Efficient biallelic knock-in in mouse embryonic stem cells by in vivo-linearization of donor and transient inhibition of DNA polymerase θ/DNA-PK

Scientific Reports, 2021
CRISPR/Cas9-mediated homology-directed repair (HDR) is used for error-free targeted knock-in of foreign donor DNA. However, the low efficiency of HDR-mediated knock-in hinders establishment of knock-in clones.
Daisuke Arai, Yoichi Nakao
doaj   +1 more source

Polθ‐mediated T‐DNA integration is a conserved mechanism across plant species

The Plant Journal, Volume 126, Issue 5, June 2026.
SUMMARY Agrobacterium‐mediated transformation remains the cornerstone of plant biotechnology, providing a versatile system for stable gene delivery and crop improvement. In Arabidopsis thaliana, DNA polymerase theta (Polθ) has been identified as a central factor mediating T‐DNA integration via theta‐mediated end joining (TMEJ).
Lejon Kralemann, Ayako Nishizawa‐Yokoi, Frank Meulewaeter, Maarten Stuiver, Anne‐Marie Kuijpers, Seiichi Toki, Paul Hooykaas, Marcel Tijsterman   +7 more
wiley   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, Volume 36, Issue 36, 4 May 2026.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

DNA break site at fragile subtelomeres determines probability and mechanism of antigenic variation in African trypanosomes. [PDF]

, 2013
Antigenic variation in African trypanosomes requires monoallelic transcription and switching of variant surface glycoprotein (VSG) genes. The transcribed VSG, always flanked by '70 bp'-repeats and telomeric-repeats, is either replaced through DNA double ...
Glover, Lucy, Horn, David, Horn, David; id_orcid, Lucy Glover (24966), Sam Alsford, David Horn (24969), Alsford, Sam, Lucy Glover, Sam Alsford (24967), David Horn   +9 more
core   +1 more source

SSA-like and MMEJ-like recombination events in a transgenic construct with in-cis programmed self-eliminating components in different E. coli strains

BMC Research Notes
Objective DNA repair pathways have been leveraged for genetic engineering strategies, including self-eliminating transgenes in insects. In effort towards establishing a self-eliminating transgenic system that leaves desired cargoes like antibodies or ...
Christian E. Ogaugwu, Zach N. Adelman
doaj   +1 more source

DNA double-strand break repair in mammals

Journal of Radiation and Cancer Research, 2017
Failure in repair of DNA double-strand breaks (DSBs) could result in various disorders in mammals including cancer. Among various exogenous agents, radiation is one of the primary causes for induction of DSBs.
Monica Pandey, Sathees C Raghavan
doaj   +1 more source

Deciphering the Evolution Pattern of Structural Variations Overlapped With Repetitive Sequence During Cattle Evolution

Advanced Science, Volume 13, Issue 28, 18 May 2026.
The authors complement bovine pan‐SV with massive novel structural variations (SVs) identified through long‐read sequencing of 83 globally distributed cattle breeds. Repetitive sequence‐mediated SVs (rep‐SV) exhibit distinct dynamic patterns throughout cattle sub‐speciation and/or domestication processes, including uneven distribution between chr‐X and
Zhifan Guo, Jinxiu Li, Adeniyi C. Adeola, Xueyan Jiang, Juntao Ma, Jian Xiao, Dexiang Hu, Kaixing Qu, Haihong Wu, Junren Chen, Zhanxing He, Tingting Yin, Ali Esmailizadeh, Jing Luo, Olivier Hanotte, Ya‐Ping Zhang, Yan Li   +16 more
wiley   +1 more source