Results 91 to 100 of about 12,345 (165)

Enhancing CRISPR/Cas‐Mediated Gene Knockout With Short Non‐Homologous Oligonucleotides

Plant Biotechnology Journal, Volume 24, Issue 6, Page 3742-3753, June 2026.
ABSTRACT Chlamydomonas reinhardtii is a model green microalga that has great industrial potential as a sustainable bio‐factory for recombinant protein and high‐value chemical production. Efficient genome editing tools are required to redesign this organism for synthetic biology applications. CRISPR‐Cas editing technologies have already been adapted for
Yen Peng Chew, Aron Ferenczi, Marie Dannay, Cristina Ponce‐Lilly, Adam Kovac, Dávid Tóth, Szilvia Z. Tóth, Attila Molnar   +7 more
wiley   +1 more source

Efficient biallelic knock-in in mouse embryonic stem cells by in vivo-linearization of donor and transient inhibition of DNA polymerase θ/DNA-PK

Scientific Reports, 2021
CRISPR/Cas9-mediated homology-directed repair (HDR) is used for error-free targeted knock-in of foreign donor DNA. However, the low efficiency of HDR-mediated knock-in hinders establishment of knock-in clones.
Daisuke Arai, Yoichi Nakao
doaj   +1 more source

Polθ‐mediated T‐DNA integration is a conserved mechanism across plant species

The Plant Journal, Volume 126, Issue 5, June 2026.
SUMMARY Agrobacterium‐mediated transformation remains the cornerstone of plant biotechnology, providing a versatile system for stable gene delivery and crop improvement. In Arabidopsis thaliana, DNA polymerase theta (Polθ) has been identified as a central factor mediating T‐DNA integration via theta‐mediated end joining (TMEJ).
Lejon Kralemann, Ayako Nishizawa‐Yokoi, Frank Meulewaeter, Maarten Stuiver, Anne‐Marie Kuijpers, Seiichi Toki, Paul Hooykaas, Marcel Tijsterman   +7 more
wiley   +1 more source

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, Volume 36, Issue 36, 4 May 2026.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

DNA break site at fragile subtelomeres determines probability and mechanism of antigenic variation in African trypanosomes. [PDF]

, 2013
Antigenic variation in African trypanosomes requires monoallelic transcription and switching of variant surface glycoprotein (VSG) genes. The transcribed VSG, always flanked by '70 bp'-repeats and telomeric-repeats, is either replaced through DNA double ...
Glover, Lucy, Horn, David, Horn, David; id_orcid, Lucy Glover (24966), Sam Alsford, David Horn (24969), Alsford, Sam, Lucy Glover, Sam Alsford (24967), David Horn   +9 more
core   +1 more source

SSA-like and MMEJ-like recombination events in a transgenic construct with in-cis programmed self-eliminating components in different E. coli strains

BMC Research Notes
Objective DNA repair pathways have been leveraged for genetic engineering strategies, including self-eliminating transgenes in insects. In effort towards establishing a self-eliminating transgenic system that leaves desired cargoes like antibodies or ...
Christian E. Ogaugwu, Zach N. Adelman
doaj   +1 more source

DNA double-strand break repair in mammals

Journal of Radiation and Cancer Research, 2017
Failure in repair of DNA double-strand breaks (DSBs) could result in various disorders in mammals including cancer. Among various exogenous agents, radiation is one of the primary causes for induction of DSBs.
Monica Pandey, Sathees C Raghavan
doaj   +1 more source

Deciphering the Evolution Pattern of Structural Variations Overlapped With Repetitive Sequence During Cattle Evolution

Advanced Science, Volume 13, Issue 28, 18 May 2026.
The authors complement bovine pan‐SV with massive novel structural variations (SVs) identified through long‐read sequencing of 83 globally distributed cattle breeds. Repetitive sequence‐mediated SVs (rep‐SV) exhibit distinct dynamic patterns throughout cattle sub‐speciation and/or domestication processes, including uneven distribution between chr‐X and
Zhifan Guo, Jinxiu Li, Adeniyi C. Adeola, Xueyan Jiang, Juntao Ma, Jian Xiao, Dexiang Hu, Kaixing Qu, Haihong Wu, Junren Chen, Zhanxing He, Tingting Yin, Ali Esmailizadeh, Jing Luo, Olivier Hanotte, Ya‐Ping Zhang, Yan Li   +16 more
wiley   +1 more source

McVey M (2010) Synthesis-dependent microhomology-mediated end joining accounts for multiple types of repair junctions

, 2020
Ku or DNA ligase 4-independent alternative end joining (alt-EJ) repair of DNA double-strand breaks (DSBs) frequently correlates with increased junctional microhomology. However, alt-EJ also produces junctions without microhomology (apparent blunt joins),
Amy Marie Yu, Mitch Mcvey
core  

The DNA helicase RTEL1 is involved in the repair of replicative DNA damage independently of the alternative end joining and the DNA–protein cross‐link repair pathways in Arabidopsis

The Plant Journal, Volume 126, Issue 3, May 2026.
SUMMARY Recent studies indicate that the Fe–S DNA helicase RTEL1 plays an important role in replicative DNA repair in plants. Since non‐homologous end joining (NHEJ) and DNA–protein cross‐link (DPC) repair also protect DNA replication in Arabidopsis, we set out to further define the interactions of RTEL1 and these pathways by analyzing double mutants ...
Lara Goldkuhle, Laura Lechner, Niklas Capdeville, Annika Dorn, Holger Puchta   +4 more
wiley   +1 more source