Results 81 to 90 of about 12,345 (165)

Compromised DNA replication in gut cells underlies sensitivity to genotoxic stress in the tardigrade Hypsibius exemplaris

The FEBS Journal, EarlyView.
DNA damage in the tardigrade Hypsibius exemplaris elicits distinct cellular outcomes depending on replication status. While non‐replicating cells tolerate genotoxic stress, constitutively replicating cells undergo irreversible replication failure upon DNA damage, leading to loss of tissue homeostasis, fat depletion, sterility, and organismal death ...
Gonzalo Quiroga‐Artigas, Pauline Fontanié, Benjamin Lacroix, Maria Dolores Molina, María Moriel‐Carretero   +4 more
wiley   +1 more source

Regulation of Error-Prone DNA Double-Strand Break Repair and Its Impact on Genome Evolution

Cells, 2020
Double-strand breaks are one of the most deleterious DNA lesions. Their repair via error-prone mechanisms can promote mutagenesis, loss of genetic information, and deregulation of the genome.
Terrence Hanscom, Mitch McVey
doaj   +1 more source

ErCas12a and T5exo-ErCas12a Mediate Simple and Efficient Genome Editing in Zebrafish

Biology, 2022
In zebrafish, RNA-guided endonucleases such as Cas9 have enabled straightforward gene knockout and the construction of reporter lines or conditional alleles via targeted knockin strategies. However, the performance of another commonly used CRISPR system,
Bingzhou Han, Yage Zhang, Yang Zhou, Biao Zhang, Christopher J. Krueger, Xuetong Bi, Zuoyan Zhu, Xiangjun Tong, Bo Zhang   +8 more
doaj   +1 more source

Gene Editing for Haemophilia—The Next Frontier

Haemophilia, EarlyView.
ABSTRACT The recently approved haemophilia A and B gene therapies via adeno‐associated virus (AAV) showed a promising therapeutic response after a single injection, but there are still limitations, including the potential loss of transgene expression and restriction in adults.
Mirko Pinotti, Gregory A. Newby, Sundar Selvaraj, Steven W. Pipe   +3 more
wiley   +1 more source

Microhomology-mediated end joining is the principal mediator of double-strand break repair during mitochondrial DNA lesions [PDF]

, 2016
Mitochondrial DNA (mtDNA) deletions are associated with various mitochondrial disorders. The deletions identified in humans are flanked by short, directly repeated mitochondrial DNA sequences; however, the mechanism of such DNA rearrangements has yet to ...
Choudhary, Bibha, Raghavan, Sathees C., Sebastian, Robin, Dahal, Sumedha, Babu, Ravi K., Raghavan, Sathees C, Babu, Ravi K, Tadi, Satish Kumar   +7 more
core   +1 more source

CRISPR-Cas-based Microhomology-Mediated End Joining for Precise Gene Replacement in Plant

bioRxiv, 2022
Abstract Precise gene or allele replacement is a desirable technology, but implementing it in plants remains challenging. CRISPR-Cas-based approaches, such as gene targeting (GT) and prime editing (PE), have opened up new possibilities for precise gene replacement in plants.
T. Vu, Gah-Hyun Lim, Seung Hee Choi, J. Y. Moon, N. T. Nguyen, Swati Das, M. Tran, Y. Sung, Jihae Kim, Y. Song, Sukweon Kim, J. Jeong, Jae-Yean Kim   +12 more
semanticscholar   +2 more sources

Arabidopsis DNA repair mutants can integrate Agrobacterium T‐DNA into the plant genome

New Phytologist, EarlyView.
Transient (a) and stable (b) transformation of Arabidopsis thaliana DNA repair/recombination mutants grown under normal light conditions. Summary Agrobacterium transfer DNA (T‐DNA) integration is mediated by plant DNA repair proteins of various nonhomologous end‐joining (NHEJ) pathways.
Lan‐Ying Lee, Yunjia Shen, Yooyoung Kim, Ayako Nishizawa‐Yokoi, Hiroaki Saika, Demi White, Wenying Liao, Stanton B. Gelvin   +7 more
wiley   +1 more source

Ionizing Radiation Induces Microhomology-Mediated End Joiningin transin Yeast and Mammalian Cells [PDF]

Radiation Research, 2009
DNA double-strand breaks repaired through nonhomologous end joining require no extended sequence homology as a template for the repair. A subset of end-joining events, termed microhomology-mediated end joining, occur between a few base pairs of homology, and such pathways have been implicated in different human cancers and genetic diseases.
Zorica, Scuric, Cecilia Y, Chan, Kurt, Hafer, Robert H, Schiestl   +3 more
openaire   +2 more sources

Progress and Challenges in Faba Bean Genetic Engineering

Legume Science, Volume 8, Issue 2, June 2026.
ABSTRACT Legumes are essential for global food security, serving as a vital source of plant‐based protein and playing a significant role in sustainable agricultural practices. Among legumes, faba bean (Vicia faba L.) is noteworthy due to its high‐protein content, broad climate adaptation, and suitability for cultivation in temperate regions.
Simone A. Gierlich, Thilani B. Jayakody, Sruthy Maria Augustine, Huijun Liu, Marko Jääskeläinen, Triin Vaisalu, Rod. J. Snowdon, Stig U. Andersen   +7 more
wiley   +1 more source

Multiple DSB Resection Activities Redundantly Promote Alternative End Joining-Mediated Class Switch Recombination

Frontiers in Cell and Developmental Biology, 2021
Alternative end joining (A-EJ) catalyzes substantial level of antibody class switch recombination (CSR) in B cells deficient for classical non-homologous end joining, featuring increased switch (S) region DSB resection and junctional microhomology (MH ...
Xikui Sun, Xikui Sun, Jingning Bai, Jingning Bai, Jiejie Xu, Jiejie Xu, Xiaoli Xi, Mingyu Gu, Mingyu Gu, Chengming Zhu, Hongman Xue, Chun Chen, Junchao Dong, Junchao Dong, Junchao Dong   +14 more
doaj   +1 more source