Protocol for microinjection of rapamycin into the zebrafish habenula [PDF]
STAR ProtocolsSummary: Mechanistic target of rapamycin complex 1 (mTorC1) activity plays a crucial role in brain development. Here, we present an approach for rapamycin microinjection into the habenula of larval zebrafish to achieve localized inhibition of the mTorC1 ...
Olga Doszyn +2 more
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Behavioral and cardiorespiratory responses to bilateral microinjections of oxytocin into the central nucleus of amygdala of Wistar rats, an experimental model of compulsion. [PDF]
PLoS ONE, 2014 INTRODUCTION:The central nucleus of amygdala plays an important role mediating fear and anxiety responses. It is known that oxytocin microinjections into the central nucleus of amygdala induce hypergrooming, an experimental model of compulsive behavior ...
Érica Maria Granjeiro +8 more
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Frontiers in Neuroscience, 2021 Mitochondria are dynamic organelles that mediate the energetic supply to cells and mitigate oxidative stress through the intricate balance of fission and fusion.
Michael Kalyn, Marc Ekker
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Multiple Sites of Associative Odor Learning as Revealed by Local Brain Microinjections of Octopamine in Honeybees [PDF]
Learning & memory (Cold Spring Harbor, N.Y.), 1998 In a classical conditioning procedure, honeybees associate an odor with sucrose resulting in the capacity of the odor to evoke an appetitive response, the extension of the proboscis (PER).
Martin Hammer, Randolf Menzel
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Microinjecting Cells Using a Constant-Flow Microinjection System [PDF]
Cold Spring Harbor Protocols, 2011 INTRODUCTIONThe efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology, and direct delivery of genetic material by microinjection remains a reliable means of transfection. In the system described here a constant flow of sample is delivered from the tip of the pipette, and the ...
David A, Dean, Joshua Z, Gasiorowski
openaire +4 more sources
The Kir channel in the nucleus tractus solitarius integrates the chemosensory system with REM sleep executive machinery for homeostatic balance [PDF]
Scientific ReportsThe locus coeruleus (LC), nucleus tractus solitarius (NTS), and retrotrapezoid nucleus (RTN) are critical chemosensory regions in the brainstem. In the LC, acid-sensing ion channels and proton pumps serve as H+ sensors and facilitate the transition from ...
Fayaz A. Mir, Sushil K. Jha
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RNAi-Mediated Silencing of Laccase 2 in Culex pipiens Pupae via Dehydration and Soaking Results in Multiple Defects in Cuticular Development [PDF]
InsectsMosquitoes transmit a range of pathogens, causing devastating effects on human health. Population genetic control strategies have been developed and successfully used for several mosquito species.
Anastasia N. Naumenko, Megan L. Fritz
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Protocol to assess the impact of sperm RNA on mouse preimplantation embryos using microinjection [PDF]
STAR ProtocolsSummary: Zygotic microinjections show that sperm RNAs transmit non-genetically inherited phenotypes to offspring and influence embryonic development.
Grace S. Lee +2 more
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“Usage a CRISPR/Cas9-based for obtaining knockouts of economically significant cattle genes” [PDF]
E3S Web of Conferences, 2023 An experimental work dealing with the gene modification using the Cas9 RNA-based editing system was performed. Point site-specific breakpoints in gDNA were introduced at the zygote stage by microinjection of spCas9 mRNA protein and guide RNAs into the ...
Bruter A. +5 more
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Journal of Cosmetic Dermatology, 2023 Melasma is a hyperpigmentary disorder causing cosmetic disfigurement. We aimed to compare the efficacy and safety of tranexamic acid (TXA) microinjections with TXA mesoneedling for facial melasma.
Nazila Poostiyan +3 more
semanticscholar +1 more source