Results 171 to 180 of about 51,233 (376)

Role of mitochondrial complex I genes in host plant expansion of Bactrocera tau (Tephritidae: Diptera) by CRISPR/Cas9 system

open access: yesInsect Science, EarlyView.
The role of 3 mitochondrial complex I gene in host expansion of Bactrocera tau was verified by CRISPR/Cas9 gene editing system. A stable homogenous strain (Btndufa7‐/‐) of B.tau was established after silencing Ndufa7. The reduced size of larvae and pupae of Ndufa7 knockout strain was first found.Then different levels of fitness and relative expression ...
Wei Shi   +3 more
wiley   +1 more source

Development of a microinjection system for RNA interference in the water flea Daphnia pulex [PDF]

open access: gold, 2013
Chizue Hiruta   +7 more
openalex   +1 more source

Optical coherence tomography guided microinjections in live mouse embryos: high-resolution targeted manipulation for mouse embryonic research

open access: yesJournal of Biomedical Optics, 2015
S. Syed   +6 more
semanticscholar   +1 more source

Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae)

open access: yesInsect Science, EarlyView.
An intronless version of the white pupae (wp) gene was engineered to restore the wild type brown puparium color in white pupae phenotype mutants of the Mediterranean fruit fly, Ceratitis capitata. Functionality of the minimal gene version (mini‐wp) was verified in vivo, as one copy of mini‐wp successfully restored the wild type phenotype in homozygous ...
Lucas Henrique Figueiredo Prates   +4 more
wiley   +1 more source

Molecular characterization of transformer, transformer‐2, and doublesex genes in the carambola fruit fly, Bactrocera carambolae, revealing potential for genetic pest management

open access: yesInsect Science, EarlyView.
Abstract Globalization and climate change may be driving the spread of the quarantine fruit fly, Bactrocera carambolae, highlighting the need for ecofriendly control methods like the Sterile Insect Technique (SIT), which relies on releasing sterile males to reduce wild populations.
Kamoltip Laohakieat   +2 more
wiley   +1 more source

High throughput microinjections of sea urchin zygotes.

open access: yesJournal of Visualized Experiments, 2014
N. Stepicheva, Jia L. Song
semanticscholar   +1 more source

Silencing of juvenile hormone‐related genes through RNA interference leads to molt failure and high mortality in the spongy moth

open access: yesInsect Science, EarlyView.
The feasibility of using RNA interference to control the globally important quarantine pest, the spongy moth. Targeting genes related to JHs play an important role in the growth and development of insects. First, the open reading frames (ORFs) of Ldjhamt and Ldjheh were identified and characterized, and the target genes were cloned and double‐stranded ...
Wenzhuai Ji   +3 more
wiley   +1 more source

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