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Implantation of Microinjected Eggs
2003Microinjected eggs are normally incubated overnight in Ml6 microdrop culture at 37°C in CO₂ until 1400 h. By this time the eggs will have developed as far as the two-cell stage. The eggs are then implanted into surrogate mothers. As the membrane covering the oviduct of the rat is much tougher than the mouse, we normally cut it with a very fine pair of ...
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Microinjection of Spermatozoa into Oocytes
Annals of the New York Academy of Sciences, 1988L, Mettler +4 more
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