Results 1 to 10 of about 145,034 (286)

A bumpy ride on the diagnostic bench of massive parallel sequencing, the case of the mitochondrial genome [PDF]

, 2014
The advent of massive parallel sequencing (MPS) has revolutionized the field of human molecular genetics, including the diagnostic study of mitochondrial (mt) DNA dysfunction.
Caljon, Ben, De Meirleir, Linda, De Paepe, Boel, Jonckheere, An, Lissens, Willy, Seneca, Sara, Smet, Joél, Spits, Claudia, Stouffs, Katrien, Van Coster, Rudy, Vancampenhout, Kim, Vanlander, Arnaud   +11 more
core   +19 more sources

Deciphering Azadirachta indica (Neem) Gum Microbiome using Metagenomic Approaches

Journal of Pure and Applied Microbiology, 2023
Indian lilac or neem (Azadirachta indica) is found in tropical and subtropical regions of the Indian subcontinent. Each part of the tree is a source of various phytochemicals. Neem gum is an exudate from mature parts of the plant stem.
Pragya Saxena, Arjun Singh, Hillol Chakdar, Sheetal Shirodkar, Alok K. Srivastava   +4 more
doaj   +1 more source

MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species [PDF]

Royal Society Open Science, 2015
We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome ...
M. Miya, Y. Sato, T. Fukunaga, T. Sado, J. Y. Poulsen, K. Sato, T. Minamoto, S. Yamamoto, H. Yamanaka, H. Araki, M. Kondoh, W. Iwasaki   +11 more
doaj   +1 more source

Characterization of the complete mitochondrial genome of the Northern Mud Gudgeon, Ophiocara porocephala (Perciformes: Eleotridae) with phylogenetic implications

Mitochondrial DNA. Part B. Resources, 2021
The first mitochondrial genome of Ophiocara porocephala was determined by the combination of next-generation sequencing (NGS) and Sanger sequencing methods. A complete circular mitogenome of O. porocephala (16,529 bp) consisted of 13 protein-coding genes,
Muhammad Hilman Fu’adil Amin, Soo Rin Lee, Bambang Irawan, Sapto Andriyono, Hyun-Woo Kim   +4 more
doaj   +1 more source

EM-mosaic detects mosaic point mutations that contribute to congenital heart disease. [PDF]

, 2020
BackgroundThe contribution of somatic mosaicism, or genetic mutations arising after oocyte fertilization, to congenital heart disease (CHD) is not well understood.
Bernstein, Daniel, Brueckner, Martina, Chung, Wendy K, DePalma, Steven, Gelb, Bruce D, Goldmuntz, Elizabeth, Gorham, Joshua M, Griffin, Emily, Hsieh, Alexander, Kim, Richard W, Lifton, Richard P, Manheimer, Kathryn B, McKean, David, Morton, Sarah U, Newburger, Jane W, Porter, George A, Qi, Hongjian, Seidman, Christine E, Seidman, JG, Shen, Yufeng, Srivastava, Deepak, Tai, Angela C, Tristani-Firouzi, Martin, Willcox, Jon AL   +23 more
core   +3 more sources

Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]

, 2020
BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer, Garber, Megan E, Hillson, Nathan J, Mukhopadhyay, Aindrila   +3 more
core   +1 more source

Ethnic Differences Shape the Alpha but Not Beta Diversity of Gut Microbiota from School Children in the Absence of Environmental Differences

Microorganisms, 2020
Although the human gut microbiome is shaped by factors such as diet, environment, and genetic background, most studies investigating the relationship between ethnicity and microbiota have compared groups living in separate geographical locations.
Ke Liu, Yongling Zhang, Qinglin Li, Huan Li, Danfeng Long, Shijuan Yan, Wenjie Huang, Ruijun Long, Xiaodan Huang   +8 more
doaj   +1 more source

Characterization of complete mitochondrial genome of Pogonophryne albipinna (Perciformes: Artedidraconidae)

Mitochondrial DNA. Part B. Resources, 2020
The complete mitochondrial genome of Pogonophryne albipinna was determined by the MiSeq platform, which was the first report in the family Artedidraconidae.
Nazia Tabassum, Md. Jobaidul Alam, Jeong-Hoon Kim, Soo Rin Lee, Ji-Hyun Lee, Hyun Park, Hyun-Woo Kim   +6 more
doaj   +1 more source

Development and Validation of a Next-Generation Sequencing Assay for BRCA1 and BRCA2 Variants for the Clinical Laboratory. [PDF]

PLoS ONE, 2015
The objective of this study was to design and validate a next-generation sequencing assay (NGS) to detect BRCA1 and BRCA2 mutations. We developed an assay using random shearing of genomic DNA followed by RNA bait tile hybridization and NGS sequencing on ...
Charles M Strom, Steven Rivera, Christopher Elzinga, Taraneh Angeloni, Sun Hee Rosenthal, Dana Goos-Root, Martin Siaw, Jamie Platt, Cory Braastadt, Linda Cheng, David Ross, Weimin Sun   +11 more
doaj   +1 more source

Design and use of a new primer pair for the characterization of the cyanobacteria Synechococcus and Prochlorococcus communities targeting petB gene through metabarcoding approaches

MethodsX, 2023
During the last years, the application of next-generation sequencing (NGS) technologies to search for specific genetic markers has become a crucial method for the characterization of microbial communities.
Alexandra Coello-Camba, Rubén Díaz-Rúa, Susana Agusti   +2 more
doaj   +1 more source