Results 21 to 30 of about 147,075 (286)
Comparison of SARS-CoV-2 sequencing using the ONT GridION and the Illumina MiSeq
Background Over 4 million SARS-CoV-2 genomes have been sequenced globally in the past 2 years. This has been crucial in elucidating transmission chains within communities, the development of new diagnostic methods, vaccines, and antivirals.
Derek Tshiabuila +12 more
doaj +1 more source
16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform [PDF]
peer-reviewedBackground Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the
Claesson, Marcus J. +4 more
core +2 more sources
QQ-SNV: single nucleotide variant detection at low frequency by comparing the quality quantiles [PDF]
Background: Next generation sequencing enables studying heterogeneous populations of viral infections. When the sequencing is done at high coverage depth ("deep sequencing"), low frequency variants can be detected.
Aerssens, Jeroen +7 more
core +4 more sources
Shifting Feeding Habits During Settlement Among Small Yellow Croakers (Larimichthys polyactis)
The small yellow croaker, Larimichthys polyactis, is a keystone species in the Yellow Sea and the East China Sea, with significant impacts on the regional ecosystem, but has experienced decades of population decline as a result of environmental changes ...
Yuange Chen +4 more
doaj +1 more source
CD-HIT-OTU-MiSeq, an Improved Approach for Clustering and Analyzing Paired End MiSeq 16S rRNA Sequences [PDF]
AbstractIn recent years, Illumina MiSeq sequencers replaced pyrosequencing platforms and became dominant in 16S rRNA sequencing. One unique feature of MiSeq technology, compared with Pyrosequencing, is the Paired End (PE) reads, with each read can be sequenced to 250-300 bases to cover multiple variable regions on the 16S rRNA gene.
Li, Weizhong, Chang, Yuanyuan
openaire +1 more source
Cost-efficient high-throughput HLA typing by MiSeq amplicon sequencing [PDF]
Abstract Background A close match of the HLA alleles between donor and recipient is an important prerequisite for successful unrelated hematopoietic stem cell transplantation. To increase the chances of finding an unrelated donor, registries recruit many hundred thousands of volunteers each ...
Lange, Vinzenz +15 more
openaire +2 more sources
The complete mitogenome of Caridina indistincta ‘sp. A’ (Decapoda: Atyidae)
A complete mitochondrial genome sequence was determined for a member of the Caridina indistincta species complex known as C. indistincta ‘sp. A’. The 15,461 bp sequence (GenBank: MH189850) was obtained via genome skimming, and contains 13 protein coding ...
Daniel J. Schmidt
doaj +1 more source
Culture-independent metagenomic sequencing of enriched agricultural water could expedite the detection and virulotyping of Shiga toxin-producing Escherichia coli (STEC).
Meghan Maguire +7 more
doaj +1 more source
The complete mitochondrial genomes of four fish species of the commercially important family Latidae were sequenced using the Illumina MiSeq, thereby significantly increasing the mitogenomic resources for the family.
Han Ming Gan +6 more
doaj +1 more source
Illumina MiSeq is the current standard for characterizing microbial communities in soil. The newer alternative, Oxford Nanopore Technologies MinION sequencer, is quickly gaining popularity because of the low initial cost and longer sequence reads ...
Bo Maxwell Stevens +3 more
doaj +1 more source

