Results 61 to 70 of about 145,034 (286)
Advanced Materials, EarlyView.A high‐throughput in vivo mRNA LNP screening platform is developed and employed to screen a large library of 122 mRNA LNPs in vivo for delivery to immune, stromal, and parenchymal cells, identifying promising LNP candidates. A novel small particle flow cytometry‐based protein adsorption analysis method is utilized to interrogate protein corona ...
Alex G. Hamilton +17 more
wiley +1 more source
Phylogenomic analysis of lactobacillus curvatus reveals two lineages distinguished by genes for fermenting plant-derived carbohydrates [PDF]
, 2018 Lactobacillus curvatus is a lactic acid bacterium encountered in many different types of fermented food (meat, seafood, vegetables, and cereals). Although this species plays an important role in the preservation of these foods, few attempts have been ...
Chaillou, Stéphane +5 more
core +2 more sources
Primer ID MiSeq Library Prep v3 [PDF]
, 2018 This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID. The Superscript III kit was used for the cDNA synthesis. We used two rounds of PCR to amplify the cDNA and incorporate Illumina indexed
openaire +1 more source
Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi
Vavilov Journal of Genetics and Breeding, 2020 Arbuscular mycorrhiza fungi (AMF) form one of the most common symbiosis with the majority of land plants. AMF supply the plant with various mineral elements, primarily phosphorus, and improve the water supply. The search for the most effective AMF strains for symbiosis and the creation of microbial preparations on that basis is an important task for ...
A. A. Kryukov +6 more
openaire +4 more sources
PrimalSeq:Generation of tiled virus amplicons for MiSeq sequencing v1 [PDF]
, 2020 Generated in collaboration by the Loman, Andersen, and Grubaugh labs. For general use of the protocol and primer design, please cite: Quick, J. et al. Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples.
Nate Matteson +5 more
openaire +1 more source
The Gut Microbiota Regulates Motor Deficits via Butyrate in a Gnal+/− Mouse Model of DYT25 Dystonia
Advanced Science, EarlyView.The present study provides compelling evidence for a modulatory role of the gut microbiota in the pathology of DYT25 dystonia, and butyrate supplementation alleviates the motor deficits of dystonia in Gnal+/− mice. Abstract Dystonia is the third most common movement disorder, following essential tremor and Parkinson's disease. The underlying mechanisms
Jingya Guo +3 more
wiley +1 more source
A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq [PDF]
PeerJ, 2016 Background The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision.
Catherine M. Burke, Aaron E. Darling
doaj +2 more sources
Experimental data from flesh quality assessment and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus labrax) fillets [PDF]
, 2019 Fresh fish are highly perishable food products and their short shelf-life limits their commercial exploitation and leads to waste, which has a negative impact on aquaculture sustainability.
Aken +19 more
core +1 more source
Barcoding PCR for MiSeq sequencing v1
, 2018 Barcoding PCR for MiSeq sequencing Barcode head 8mer-GCT ATG CGC GAG CTG C Modified from Rudi et al. (2003)
Eva Petrova, Roey Angel
openaire +1 more source
Advanced Science, EarlyView.A solid‐state fermented probiotic (PYW) is developed using wheat bran as substrate, containing high viable Lacticaseibacillus paracasei and bioactive metabolites. PYW alleviates intestinal inflammation by gut microbiota composition, enriching indole derivatives, activating aryl hydrocarbon receptor signaling, and regulating macrophage polarization. PYW
Heng Zhang +11 more
wiley +1 more source