Results 71 to 80 of about 67,036 (249)

A novel dilated contextual attention module for breast cancer mitosis cell detection

Frontiers in Physiology
Background and object: Mitotic count (MC) is a critical histological parameter for accurately assessing the degree of invasiveness in breast cancer, holding significant clinical value for cancer treatment and prognosis.
Zhiqiang Li, Xiangkui Li, Weixuan Wu, He Lyu, Xuezhi Tang, Chenchen Zhou, Fanxin Xu, Bin Luo, Yulian Jiang, Xingwen Liu, Wei Xiang   +10 more
doaj   +1 more source

Tunable Bioresorbable Scaffolds With Marine Sulfated Polysaccharides for Small‐Caliber Vascular Grafts: A Multi‐Layered Strategy Combining Electrospinning and 4‐Axis Printing

Advanced Healthcare Materials, EarlyView.
A multilayered small‐caliber vascular scaffold combining electrospinning and 4‐axis printing is developed and biofunctionalized with marine sulfated polysaccharides from Holothuria tubulosa. The resulting construct exhibits enhanced hemocompatibility, tunable mechanical properties, and supports endothelial and smooth muscle cell adhesion and ...
Gabriele Obino, Alberto Sensini, Tim ten Brink, Gabriele Nieddu, Tristan Bodet, Giovanni Andrea Deiana, Martijn van Griensven, Marilena Formato, Antonio J. Lepedda, Lorenzo Moroni   +9 more
wiley   +1 more source

Mitosis detection, fast and slow: Robust and efficient detection of mitotic figures

Medical Image Analysis
Counting of mitotic figures is a fundamental step in grading and prognostication of several cancers. However, manual mitosis counting is tedious and time-consuming. In addition, variation in the appearance of mitotic figures causes a high degree of discordance among pathologists.
Mostafa Jahanifar, Adam Shephard, Neda Zamanitajeddin, Simon Graham, Shan E. Ahmed Raza, Fayyaz Minhas, Nasir Rajpoot   +6 more
openaire   +3 more sources

Two‐Photon 3D Printing of Functional Microstructures Inside Living Cells

Advanced Materials, EarlyView.
This study demonstrates 3D printing inside living cells by using two‐photon photo‐lithography. A bio‐compatible photoresist is injected into cells and selectively polymerized with a femtosecond laser, creating custom‐shaped intracellular structures with submicron resolution.
Maruša Mur, Aljaž Kavčič, Uroš Jagodič, Rok Podlipec, Matjaž Humar   +4 more
wiley   +1 more source

Detection of Anaphase Bridge Formation by Immunofluorescence Microscopy in Mammalian Cells

Bio-Protocol, 2016
The aim of this protocol is to provide a comprehensive description of the materials, equipment and reproducible methods to detect and analyze anaphase bridges in immunofluorescence microscopy using DAPI to detect cells that failed to completely segregate
Thomas Aschacher, Florian Enzmann
doaj   +1 more source

Femtosecond‐Laser‐Fabricated 3D CYTOP Microfluidic Chips Enabling Super‐Resolution Live Imaging of Cell Behavior in Confined Microspaces

Advanced Materials Technologies, EarlyView.
Using two‐photon polymerization of SU‐8 together with mold processing allowed the creation of defect‐free, flexible 3D microfluidic structures with sub‐micrometer precision in CYTOP. The fabricated CYTOP microfluidic chips enabled super‐resolution imaging of cancer cells in the microchannels, clearly visualizing previously undetectable fine structural ...
Koji Sugioka, Mirai Hanzawa, Kotaro Obata, Sota Matsumoto, Masatoshi Takagi, Asako Sakaue‐Sawano, Asako Tosaki, Satoshi Shimozono, Felix Sima, Hiroyuki Kawano, Takuro Tojima, Daisuke Miyashiro, Kazunari Ozasa, Yasutaka Hanada, Godai Miyaji, Akihiko Nakano, Atsushi Miyawaki   +16 more
wiley   +1 more source

Artificial intelligence-based automated determination in breast and colon cancer and distinction between atypical and typical mitosis using a cloud-based platform

Pathology and Oncology Research
Artificial intelligence (AI) technology in pathology has been utilized in many areas and requires supervised machine learning. Notably, the annotations that define the ground truth for the identification of different confusing process pathologies, vary ...
Nilay Bakoglu, Emine Cesmecioglu, Hirotsugu Sakamoto, Masao Yoshida, Takashi Ohnishi, Seung-Yi Lee, Lindsey Smith, Yukako Yagi   +7 more
doaj   +1 more source

Detection of large extracellular silver nanoparticle rings observed during mitosis using darkfield microscopy.

PLoS ONE, 2020
During studies on the absorption and interactions between silver nanoparticles and mammalian cells grown in vitro it was observed that large extracellular rings of silver nanoparticles were deposited on the microscope slide, many located near post ...
Robert M Zucker, Jayna Ortenzio, Laura L Degn, William K Boyes   +3 more
doaj   +1 more source

Mitosis Detection from Pathology Images

, 2021
In the case of breast cancer, according to the Nottingham Grading System, counting mitotic cells is an important indicator of tumour diagnosis and grading. Pathologists usually manually count mitosis from histopathology images to determine the cancer grade. This is a challenging and time-consuming procedure.
openaire   +1 more source

ROS Activated NETosis of Bone Marrow CD55+ Intermediate Mature Neutrophils Through HIF1α‐PADI4 Pathway to Initiate Bone Aging

Advanced Science, EarlyView.
In this study, we find CD55+ neutrophils show activated NETosis within bone marrow, induce BMSC senescence and osteogenesis inhibition, finally leading to bone aging initiation. Mechanistically, ROS synergizes with the CD55‐driven HIF1α‐PADI4 pathway to promote NETosis.
Yutong Guo, Shengjie Cui, Xi Wen, Yidi Wang, Ben Wu, Yixiang Wang, Yan Gu   +6 more
wiley   +1 more source