Results 231 to 240 of about 4,754,428 (281)
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Mixed-mode chromatography of nucleic acids
Chemical Reviews, 1989Larry W McLaughlin
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Journal of Chromatography A, 2012 Selection of a first-dimensional separation method is very important in proteomic studies performed with two-dimensional liquid chromatography (2D-LC), since reversed-phase chromatography (RPLC) is usually chosen as the second-dimensional separation ...
Wei Lai, Zhen Long, Xiuli Zhang
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Modeling of mixed-mode chromatography of peptides
Journal of Chromatography A, 2013Mixed-mode chromatographic materials are more and more often used for the purification of biomolecules, such as peptides and proteins. In many instances they in fact exhibit better selectivity values and therefore improve the purification efficiency compared to classical materials.
S. Bernardi +3 more
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Isoform separation of proteins by mixed-mode chromatography
Protein Expression and Purification, 2015Mixed-mode chromatography uses a multimodal functional resin, mainly composed of electrostatic and aromatic/hydrophobic groups. Here we have tested 2 mixed-mode resins, anion-exchange Capto adhere and cation-exchange Capto MMC, using 2 model proteins, i.e., an Fc-fusion etanercept, and bovine serum albumin (BSA). When etanercept was produced in Chinese
Tsutomu, Arakawa +2 more
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One step purification of chymosin by mixed mode chromatography
Biotechnology and Bioengineering, 1997Mixed mode Sepharose and Perloza bead cellulose matrices were prepared using various chemistries. These matrices contained hydrophobic (aliphatic and/or aromatic) and ionic (carboxylate or alkylamine) groups. Hydrophobic amine ligands were attached to epichlorohydrin activated Sepharose (mixed mode amine matrices).
S C, Burton, N W, Haggarty, D R, Harding
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Development and application of separation materials for mixed-mode chromatography
Chinese Journal of Chromatography, 2013Mixed-mode chromatography has received more and more attention due to its unique chromatographic characteristics recently. In this review, a summary of the development and applications of mixed-mode chromatography is presented. According to the types of hydrophilic interaction/ion-exchange mixed-mode chromatography (HILIC/IEX), reversed-phase ...
Xuefang, Dong +5 more
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Nucleic acid resolution by mixed-mode chromatography
Journal of Chromatography A, 1984Two approaches to the preparation of mixed-mode (ionic—hydrophobic) supports for high-performance liquid chromatography are described. In the first, hydrophobic moieties are covalently bound to an anion-exchange support (ASP-Hypersil). In the second approach ionic amines are bound through hydrophobic interactions to a reversed-phase support (ODS ...
Rainer Bischoff, Larry W. McLaughlin
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Analysis and purification of IgG4 bispecific antibodies by a mixed-mode chromatography
Analytical Biochemistry, 2015Therapeutic non-hinge-modified IgG4 molecules form bispecific hybrid antibodies with endogenous human IgG4 molecules via a process known as Fab-arm exchange (or called half molecule exchange). Analysis of the bispecific hybrids is critical for studies of half molecule exchange. A number of analytical methods are available to detect IgG4 hybrids.
Xiaoyu, Yang +6 more
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Capto MMC mixed-mode chromatography of murine and rabbit antibodies
Protein Expression and Purification, 2016Murine antibodies have weak affinity for Protein-A. Here, we have tested binding of murine monoclonal antibody (mAb) to Protein-A or Protein-A/Protein-G mixture under salting-out conditions. The addition of ammonium sulfate to HEK conditioned medium (CM) expressing murine mAb resulted in complete binding, leading to its elution by low pH or neutral ...
Tsutomu, Arakawa +5 more
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