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Mixed Mode Chromatography: A Novel Way Toward New Selectivity
Mixed mode chromatography offers a diversity of ligands, each providing a new selectivity. This allows the design of novel purification processes with reduced column steps. Structure of ligands is based on both hydrophobic and ionic groups. Thanks to its salt tolerance, crude extracts or post-IEX samples can be loaded directly without conditioning ...
Santarelli, Xavier, Cabanne, Charlotte
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Review on the Application of Mixed-mode Chromatography for Separation of Structure Isoforms
Proteins often generate structure isoforms naturally or artificially due to, for example, different glycosylation, disulfide scrambling, partial structure rearrangement, oligomer formation or chemical modification. The isoform formations are normally accompanied by alterations in charged state or hydrophobicity.
T. Arakawa
semanticscholar +4 more sources
Mixed-Mode Chromatography and Its Applications to Biopolymers
Journal of Chromatography A, 2011Mixed-mode chromatography is a type of chromatography in which a chromatographic stationary phase interacts with solutes through more than one interaction mode. This technique has been growing rapidly because of its advantages over conventional chromatography, such as its high resolution, high selectivity, high sample loading, high speed, and the ...
Xindu Geng
exaly +3 more sources
Journal of Chromatography A
Hydrophilic Interaction Liquid Chromatography (HILIC) and Mixed-Mode Chromatography (MMC) excel in separating polar, hydrophilic, and charged analytes due to unique hydrophilic or mixed-mode retention mechanisms. They represent a complementary approach to the widely used Reversed Phase Liquid Chromatography (RPLC).
Kelly Zhang, Bingchuan Wei, Lulu Dai
exaly +4 more sources
Hydrophilic Interaction Liquid Chromatography (HILIC) and Mixed-Mode Chromatography (MMC) excel in separating polar, hydrophilic, and charged analytes due to unique hydrophilic or mixed-mode retention mechanisms. They represent a complementary approach to the widely used Reversed Phase Liquid Chromatography (RPLC).
Kelly Zhang, Bingchuan Wei, Lulu Dai
exaly +4 more sources

