Results 251 to 260 of about 4,947,794 (321)

pH-dependent selective separation of acidic and basic proteins using quaternary ammoniation functionalized cysteine-zwitterionic stationary phase with RPLC/IEC mixed-mode chromatography.

Talanta: The International Journal of Pure and Applied Analytical Chemistry, 2021
In this paper, a cysteine-functionalized zwitterionic stationary phase (Cys-silica) was prepared based on the "thiol-ene" click chemistry between cysteine and vinyl-functionalized silica, and was further modified with bromoethane, 1-bromooctane and 1 ...
Jian-Zhong Wang, Jian-Shan Wang, X. Ning, Jia-Wei Liu, Hong-Jun Xia, Guang-Ping Wan, Q. Bai   +6 more
semanticscholar   +1 more source

Prospects of using hyperbranched stationary phase based on poly(styrene-divinylbenzene) in mixed-mode chromatography.

Journal of Chromatography A, 2021
Evaluation of the chromatographic properties of covalently bonded hyperbranched stationary phase based on poly(styrene-divinylbenzene) (PS-DVB) and containing zwitterionic fragments in the structure of functional layer was conducted in suppressed ion ...
A. Popov, K. A. Spiridonov, A. S. Uzhel, A. D. Smolenkov, A. Chernobrovkina, A. Zatirakha   +5 more
semanticscholar   +1 more source

Preparation of two amide bonded stationary phases and comparative evaluation under mixed-mode chromatography.

Journal of Separation Science, 2021
In this work, the conventional reactions were used to functionalize the silica surface with amide and hydrocarbon chain groups affording two different mixed-mode stationary phases (Sil-amide-C11 and Sil-C12-amide).
E. Hosseini, K. Tabar-Heydar
semanticscholar   +1 more source

Application of pH-salt dual gradient elution in purifying a WuXiBody-based bispecific antibody by MMC ImpRes mixed-mode chromatography.

Protein Expression and Purification, 2021
WuXiBody is a novel bispecific antibody (bsAb) platform developed by WuXi Biologics. BsAbs based on WuXiBody can adopt either asymmetric or symmetric format.
Yan Wan, Yu-Meng Wang, Ting-mo Zhang, Sheng-Wei Zhang, Ying Wang, Yifeng Li   +5 more
semanticscholar   +1 more source

Purification of N-acetylgalactosamine-modified-oligonucleotides using orthogonal anion-exchange and mixed-mode chromatography approaches.

Journal of Chromatography A, 2021
N-acetylgalactosamine (GalNAc)-modified small interfering ribonucleic acids (siRNA) have shown promising outcomes for targeted siRNA delivery resulting in gene silencing in vivo; however, their structural complexity requires development of new ...
A. A. Kazarian, Wesley W. Barnhart, J. Long, K. Sham, Bin Wu, Justin K. Murray   +5 more
semanticscholar   +1 more source

Modeling of mixed-mode chromatography of peptides

Journal of Chromatography A, 2013
Mixed-mode chromatographic materials are more and more often used for the purification of biomolecules, such as peptides and proteins. In many instances they in fact exhibit better selectivity values and therefore improve the purification efficiency compared to classical materials.
S. Bernardi, D. Gétaz, N. Forrer, MORBIDELLI, MASSIMO   +3 more
openaire   +2 more sources

Isoform separation of proteins by mixed-mode chromatography

Protein Expression and Purification, 2015
Mixed-mode chromatography uses a multimodal functional resin, mainly composed of electrostatic and aromatic/hydrophobic groups. Here we have tested 2 mixed-mode resins, anion-exchange Capto adhere and cation-exchange Capto MMC, using 2 model proteins, i.e., an Fc-fusion etanercept, and bovine serum albumin (BSA). When etanercept was produced in Chinese
Tsutomu, Arakawa, Sean, Ponce, Glen, Young   +2 more
openaire   +2 more sources

Mixed-mode chromatography characteristics of chiralpak ZWIX(+) and ZWIX(-) and elucidation of their chromatographic orthogonality for LC × LC application.

Analytica Chimica Acta, 2020
Two-dimensional liquid chromatography requires orthogonal columns and/or separation principles in the first and second separation dimension. It is sometimes not straightforward to achieve. Chiral columns could expand the toolbox for 2D-LC, but are rarely
Stefanie Bäurer, Martina Ferri, A. Carotti, S. Neubauer, R. Sardella, M. Lämmerhofer   +5 more
semanticscholar   +1 more source

Preparation of small peptoid-modified zwitterion-exchange/reversed-phase mixed-mode chromatography stationary phases for the separation of arenes and antibiotics.

Analytica Chimica Acta
In this study, three small peptoids with different structures, named Sil-peptoids, were developed to improve the separation selectivity of zwitterion-exchange/reversed-phase mixed-mode chromatography stationary phases for multi-component complex drugs ...
Meng Xu, Zhenwei Wu
semanticscholar   +1 more source

Preparation of an aminophenylboronic acid and N-isopropyl acrylamide copolymer functionalized stationary phase for mixed-mode chromatography.

Journal of Chromatography A, 2020
A novel stationary phase co-modified with N-isopropyl acrylamide (NIPAM) and 3-aminophenylboronic acid copolymer on the silica was synthesized through atom transfer radical polymerization (ATRP) reaction for performing mixed-mode and boronate affinity ...
Di Zhou, Qiurong Luo, Qinbei Zeng, Yunchao Zheng, Xiujun Ren, Die Gao, Qi-Feng Fu, Kailian Zhang, Zhi-Ning Xia, Lu-Jun Wang   +9 more
semanticscholar   +1 more source