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Controlled mixed-mode interaction chromatography on membrane adsorbers
Journal of Chromatography A, 1996Abstract Membrane adsorbers (MAs) are used for protein separation in controlled mixed-mode interaction chromatography. The strong anion- and cation-exchange MAs used are made either from a synthetic copolymer or from modified cellulose membranes. The affinity MAs (Cibacron Blue) are also made from the copolymer membranes.
Ruth Freitag +2 more
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IgG Aggregate Removal by Charged-Hydrophobic Mixed Mode Chromatography
Current Pharmaceutical Biotechnology, 2009Charged-hydrophobic mixed mode chromatography methods have been applied to antibody purification for decades and have focused more recently on the specific task of aggregate removal. They exploit various combinations of alkyl and aromatic hydrophobic groups with positively and/or negatively charged residues.
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Improved purification of immunoglobulin G from plasma by mixed‐mode chromatography
Journal of Separation Science, 2014Efficient loading of immunoglobulin G in mixed‐mode chromatography is often a serious bottleneck in the chromatographic purification of immunoglobulin G. In this work, a mixed‐mode ligand, 4‐(1H‐imidazol‐1‐yl) aniline, was coupled to Sepharose Fast Flow to fabricate AN SepFF adsorbents with ligand densities of 15–64 mmol/L, and the chromatographic ...
Dong-Sheng, Chai +3 more
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Journal of Chromatography B, 2019
The main aim of this study was to define the optimal adsorption and elution conditions for the purification of human immunoglobulin G (IgG) by mixed-mode chromatography using the multimodal resin Capto MMC. To this end, Central Composite Experimental Design (ED) was performed for both the adsorption and desorption stages.
P L R, de Sousa +8 more
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The main aim of this study was to define the optimal adsorption and elution conditions for the purification of human immunoglobulin G (IgG) by mixed-mode chromatography using the multimodal resin Capto MMC. To this end, Central Composite Experimental Design (ED) was performed for both the adsorption and desorption stages.
P L R, de Sousa +8 more
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Recovery and purification of a Kluyvermyces lactis β -galactosidase by Mixed Mode Chromatography
Journal of Chromatography B, 2016Mixed Mode Chromatography (MMC) is a potential separation technique that allows simultaneous ionic and hydrophobic interactions between the adsorbent and the adsorbate. The aim of this work was to assess the recovery and purification of a Kluyveromyces lactis β-galactosidase employing MMC.
Micael de Andrade, Lima +3 more
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Review on the Application of Mixed-mode Chromatography for Separation of Structure Isoforms
Current Protein & Peptide Science, 2018Proteins often generate structure isoforms naturally or artificially due to, for example, different glycosylation, disulfide scrambling, partial structure rearrangement, oligomer formation or chemical modification. The isoform formations are normally accompanied by alterations in charged state or hydrophobicity.
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Journal of Chromatography A
Hydrophilic Interaction Liquid Chromatography (HILIC) and Mixed-Mode Chromatography (MMC) excel in separating polar, hydrophilic, and charged analytes due to unique hydrophilic or mixed-mode retention mechanisms. They represent a complementary approach to the widely used Reversed Phase Liquid Chromatography (RPLC).
Bingchuan Wei, Lulu Dai, Kelly Zhang
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Hydrophilic Interaction Liquid Chromatography (HILIC) and Mixed-Mode Chromatography (MMC) excel in separating polar, hydrophilic, and charged analytes due to unique hydrophilic or mixed-mode retention mechanisms. They represent a complementary approach to the widely used Reversed Phase Liquid Chromatography (RPLC).
Bingchuan Wei, Lulu Dai, Kelly Zhang
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ANIONIC EXCHANGE-HYDROPHOBIC MIXED MODE CHROMATOGRAPHY RESIN
2021BELISLE CHRISTOPHER +4 more
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PURIFICATION OF ANTIBODIES BY MIXED MODE CHROMATOGRAPHY
2022FALKENSTEIN ROBERTO, KONRAD SUSANNE
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