Results 241 to 250 of about 4,947,794 (321)

Ligands for Mixed-Mode Protein Chromatography: Principles, Characteristics and Design

Journal of Biotechnology, 2009
Mixed-mode chromatography is a chromatographic method that utilizes more than one form of interactions between the stationary phase and the solutes in a feed stream. Compared with other types of chromatography, mixed-mode chromatography is advantageous in its salt-independent adsorption, facile elution by charge repulsion, and unique selectivity. Hence,
Yan Sun, Guofeng Zhao
exaly   +3 more sources

Antibody Aggregate Removal Using a Mixed-Mode Chromatography Resin

Methods in molecular biology, 2020
In monoclonal antibody (mAb) production, aggregates represent a major class of product-related impurities that needs to be removed by the downstream process. Protein A chromatography is generally less effective at removing antibody aggregates under typical conditions, and in most cases aggregate removal relies on a subsequent polishing chromatography ...
Tao, Chen, Gaili, Guo, Guoqing, Tan, Ying, Wang, Yifeng, Li   +4 more
openaire   +3 more sources

Preparation and evaluation of dextran-grafted mixed-mode chromatography adsorbents

Journal of Chromatography A, 2019
In the present work, mixed-mode chromatography adsorbents grafted with dextran of different molecular weight were prepared. Compared to non-grafted adsorbent (BA-6B), the morphology of dextran-grafted adsorbents (BA-6B-Ts) did not change significantly, while the water content (ω), porosity (P) and pore volume (V) decreased.
Jiali, Gu, Yan, Zhang, Hongfei, Tong, Yang, Liu, Laiyu, Sun, Yajun, Wang, Li, Xiao   +6 more
openaire   +3 more sources

Mixed Mode Chromatography, Complex Development for Large Opportunities

Current Protein & Peptide Science, 2018
Mixed mode chromatography resins with salt tolerance, large design space and orthogonal selectivity requires a slightly more complex development than traditional resins. It is important to screen several ligands and several binding and elution conditions. This allows taking full advantage of these resins.
Cabanne, Charlotte, Santarelli, Xavier
openaire   +3 more sources

Mixed-Mode Chromatography of Nucleic Acids

Chemical Reviews, 1989
Larry W McLaughlin
exaly   +2 more sources

Recent Advances in Materials for Stationary Phases of Mixed-Mode High-Performance Liquid Chromatography

TrAC - Trends in Analytical Chemistry, 2016
Mixed-mode high-performance liquid chromatography (MHPLC) is a type of chromatographic technique with extraordinary separation properties, such as adjustable selectivity, high resolution and high sample loading capacity.
Weili Wei, Xu Jie, Lujun Wang
exaly   +2 more sources

Monomer-mediated fabrication of microporous organic network@silica microsphere for reversed-phase/hydrophilic interaction mixed-mode chromatography.

Talanta: The International Journal of Pure and Applied Analytical Chemistry, 2022
Microporous organic networks (MONs) are promising in high performance liquid chromatography (HPLC) with large specific surface area, good hydrophobicity and stability.
Hao-Fei Sun, Yuan-Yuan Cui, Chang-Qing Zhen, Cheng-Xiong Yang   +3 more
semanticscholar   +1 more source

Two-Dimensional Liquid Chromatography Separation of Peptides Using Reversed-Phase/Weak Cation-Exchange Mixed-Mode Column in First Dimension

Journal of Chromatography A, 2012
Selection of a first-dimensional separation method is very important in proteomic studies performed with two-dimensional liquid chromatography (2D-LC), since reversed-phase chromatography (RPLC) is usually chosen as the second-dimensional separation ...
Wei Lai, Zhen Long, Xiuli Zhang
exaly   +2 more sources

Hydrophilic Interaction/Weak Cation-Exchange Mixed-Mode Chromatography for Chitooligosaccharides Separation

Carbohydrate Research, 2012
A hydrophilic interaction/weak cation-exchange (HILIC/WCX) mixed-mode chromatography was developed for separation of chitooligosaccharides (COS) using a weak cation-exchange column. For better understanding of the retention characteristics of the column,
Wei Lai, Shen Aijin, Xuefang Dong
exaly   +2 more sources

Striped covalent organic frameworks modified stationary phase for mixed mode chromatography.

Journal of Chromatography A, 2021
Covalent organic frameworks (COFs) have showed expected potential in chromatographic separation due to unique structure and excellent performance. Nowadays, COF materials applied as chromatographic stationary phases is still in its infancy.
Yunchao Zheng, Mei Wan, Jingqiu Zhou, Qiurong Luo, Die Gao, Qi-Feng Fu, Jing Zeng, Fengjiao Zu, Lu-Jun Wang   +8 more
semanticscholar   +1 more source