Results 261 to 270 of about 4,947,794 (321)
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Journal of Chromatography A, 2020
Until today, ion-pair reversed-phase chromatography is still the dominating method for analytical characterization of synthetic oligonucleotides. Its hyphenation with mass spectrometry, however, has some drawbacks such as ion-suppression in electrospray ...
Feiyang Li +3 more
semanticscholar +1 more source
Until today, ion-pair reversed-phase chromatography is still the dominating method for analytical characterization of synthetic oligonucleotides. Its hyphenation with mass spectrometry, however, has some drawbacks such as ion-suppression in electrospray ...
Feiyang Li +3 more
semanticscholar +1 more source
One step purification of chymosin by mixed mode chromatography
Biotechnology and Bioengineering, 1997Mixed mode Sepharose and Perloza bead cellulose matrices were prepared using various chemistries. These matrices contained hydrophobic (aliphatic and/or aromatic) and ionic (carboxylate or alkylamine) groups. Hydrophobic amine ligands were attached to epichlorohydrin activated Sepharose (mixed mode amine matrices).
S C, Burton, N W, Haggarty, D R, Harding
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Talanta: The International Journal of Pure and Applied Analytical Chemistry
Ionic liquids have garnered significant attention in the development of stationary phases for high-performance liquid chromatography (HPLC) due to their core characteristics such as wide electrochemical windows, high ionic conductivity, and strong ...
Wei-Ran Sun +8 more
semanticscholar +1 more source
Ionic liquids have garnered significant attention in the development of stationary phases for high-performance liquid chromatography (HPLC) due to their core characteristics such as wide electrochemical windows, high ionic conductivity, and strong ...
Wei-Ran Sun +8 more
semanticscholar +1 more source
Protein Expression and Purification, 2020
WuXiBody is a novel bispecific antibody (bsAb) platform developed by WuXi Biologics. Its key feature is the replacement of one parental mAb's CH1/CL constant region with the T cell receptor (TCR) constant domain, a design aimed at promoting cognate heavy
Yan Wan +4 more
semanticscholar +1 more source
WuXiBody is a novel bispecific antibody (bsAb) platform developed by WuXi Biologics. Its key feature is the replacement of one parental mAb's CH1/CL constant region with the T cell receptor (TCR) constant domain, a design aimed at promoting cognate heavy
Yan Wan +4 more
semanticscholar +1 more source
Development and application of separation materials for mixed-mode chromatography
Chinese Journal of Chromatography, 2013Mixed-mode chromatography has received more and more attention due to its unique chromatographic characteristics recently. In this review, a summary of the development and applications of mixed-mode chromatography is presented. According to the types of hydrophilic interaction/ion-exchange mixed-mode chromatography (HILIC/IEX), reversed-phase ...
Xuefang, Dong +5 more
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Nucleic acid resolution by mixed-mode chromatography
Journal of Chromatography A, 1984Two approaches to the preparation of mixed-mode (ionic—hydrophobic) supports for high-performance liquid chromatography are described. In the first, hydrophobic moieties are covalently bound to an anion-exchange support (ASP-Hypersil). In the second approach ionic amines are bound through hydrophobic interactions to a reversed-phase support (ODS ...
Rainer Bischoff, Larry W. McLaughlin
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Analysis and purification of IgG4 bispecific antibodies by a mixed-mode chromatography
Analytical Biochemistry, 2015Therapeutic non-hinge-modified IgG4 molecules form bispecific hybrid antibodies with endogenous human IgG4 molecules via a process known as Fab-arm exchange (or called half molecule exchange). Analysis of the bispecific hybrids is critical for studies of half molecule exchange. A number of analytical methods are available to detect IgG4 hybrids.
Xiaoyu, Yang +6 more
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Protein Expression and Purification, 2019
During recombinant production of asymmetric IgG-like bispecific antibodies (bsAbs), various by-products are often observed due to unbalanced chain expression and incorrect chain pairing.
Jiaqin Tang +4 more
semanticscholar +1 more source
During recombinant production of asymmetric IgG-like bispecific antibodies (bsAbs), various by-products are often observed due to unbalanced chain expression and incorrect chain pairing.
Jiaqin Tang +4 more
semanticscholar +1 more source
Analysis of monoclonal antibody oxidation by simple mixed mode chromatography
Journal of Chromatography A, 2016Analysis of oxidation of monoclonal antibodies (mAbs) in most cases relies on peptide mapping and LC-MS, which is time consuming and labor-intensive. A robust chromatography based method that is able to resolve and quantitate mAb oxidation variants due to oxidized methionine or tryptophan is highly desired.
Jorge Alexander Pavon +9 more
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Capto MMC mixed-mode chromatography of murine and rabbit antibodies
Protein Expression and Purification, 2016Murine antibodies have weak affinity for Protein-A. Here, we have tested binding of murine monoclonal antibody (mAb) to Protein-A or Protein-A/Protein-G mixture under salting-out conditions. The addition of ammonium sulfate to HEK conditioned medium (CM) expressing murine mAb resulted in complete binding, leading to its elution by low pH or neutral ...
Tsutomu, Arakawa +5 more
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