Results 271 to 280 of about 4,947,794 (321)
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Session 3.1: Protein A, Hydroxyapatite, and Mixed-Mode Chromatography
PDA Journal of Pharmaceutical Science and Technology, 2016In addition to removing process/product impurities such as host cellular protein (HCP), DNA, and aggregates, chromatography unit operations can also contribute to overall viral clearance capacity.
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Mixed-Mode Chromatography and Its Role in Monoclonal Antibody Purification
2023As the biopharmaceutical industry matures and embraces process intensification methodologies allied to the emergence of newer personalized medicines, a key constant is the regulatory need to purify products that satisfy the criteria of safety, quality, and efficacy in each batch of released product destined for clinical use.
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Protein Expression and Purification, 2019
We previously developed a method that can significantly improve Protein A chromatography's capability of removing antibody aggregates. That particular method requires polyethylene glycol (PEG) and 400 mM or more of calcium chloride/sodium chloride to be ...
Yuan Zhang +3 more
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We previously developed a method that can significantly improve Protein A chromatography's capability of removing antibody aggregates. That particular method requires polyethylene glycol (PEG) and 400 mM or more of calcium chloride/sodium chloride to be ...
Yuan Zhang +3 more
semanticscholar +1 more source
Separation and Purification Technology, 2018
Adsorption selectivity is one of the most important parameters for chromatography. As a promising technology for protein purification, mixed-mode chromatography (MMC) has unique advantages; however, its selectivity needs to be improved further.
Wei Shi +4 more
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Adsorption selectivity is one of the most important parameters for chromatography. As a promising technology for protein purification, mixed-mode chromatography (MMC) has unique advantages; however, its selectivity needs to be improved further.
Wei Shi +4 more
semanticscholar +1 more source
Polydopamine‐functionalized monolithic silica column for mixed‐mode chromatography†
Journal of Separation Science, 2013Dopamine is easy to self‐polymerize under alkaline conditions and the resultant polydopamine is easy to adhere to the surface of many organic and inorganic materials. Based on the characteristics of dopamine, in this paper, a new polydopamine functionalized monolithic silica column was successfully prepared for performing mixed‐mode chromatography. The
Qiaoli, Zhou +3 more
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Journal of Chromatography A, 2008
A mixed-mode chromatographic (MMC) sorbent was prepared by functionalizing the silica sorbent with a pentafluorophenyl (PFP) ligand. The resulting stationary phase provided a reversed-phase (RP) retention mode along with a relatively mild strong cation-exchange (SCX) retention interaction. While the mechanism of interaction is not entirely clear, it is
Martin, Gilar +4 more
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A mixed-mode chromatographic (MMC) sorbent was prepared by functionalizing the silica sorbent with a pentafluorophenyl (PFP) ligand. The resulting stationary phase provided a reversed-phase (RP) retention mode along with a relatively mild strong cation-exchange (SCX) retention interaction. While the mechanism of interaction is not entirely clear, it is
Martin, Gilar +4 more
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Purification process of recombinant monoclonal antibodies with mixed mode chromatography
Journal of Chromatography A, 2015An innovative process to purify mAb from CHO cell culture supernatant was developed. This three-step process involved two mixed mode resins and an anion exchange membrane. We used a human IgG mixture to determine the optimal conditions for each purification step.
Maria, Sophie +6 more
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Biochemical engineering journal, 2019
Advance chromatin extraction, based on a fatty acid/allantoin/solid phase system, has been reported to significantly improve the purification performance of protein A chromatography, cation exchange chromatography and electronegative mixed-mode ...
Wenshuai Liu +9 more
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Advance chromatin extraction, based on a fatty acid/allantoin/solid phase system, has been reported to significantly improve the purification performance of protein A chromatography, cation exchange chromatography and electronegative mixed-mode ...
Wenshuai Liu +9 more
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Two-dimensional liquid chromatography with mixed mode stationary phases
Journal of Chromatography A, 2005Mixed mode stationary phases with ion-pairing reagent (acidic or basic) as integral part of hydrophobic chain offers unique selectivity, and hence, are ideal for multidimensional separations. The retention of hydrophobic components is a function of organic content, whereas that of charged species is a function of organic content, ionogenic modifier and
Cadapakam J, Venkatramani +1 more
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