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Metagenomic next-generation sequencing for tuberculosis diagnosis: enhanced performance and cost-effectiveness. [PDF]

Microbiol Spectr
Zhou X, Wei G, Song T, Yu Y, Chen J, Long J, Tao X, Zhang J, Jiang L.   +8 more
europepmc   +1 more source

mNGS in clinical microbiology laboratories: on the road to maturity

Critical Reviews in Microbiology, 2019
Metagenomic next-generation sequencing (mNGS) is increasingly being applied in clinical laboratories for unbiased culture-independent diagnosis. Whether it can be a next routine pathogen identification tool has become a topic of concern. We review the current implementation of this new technology for infectious disease diagnostics and discuss the ...
Jinming Li, Dongsheng Han, Ping Tan
exaly   +4 more sources

The diagnostic value of RNA-mNGS and DNA-mNGS in differentiating bacterial infection from colonization in the lower respiratory tract [PDF]

Frontiers in Cellular and Infection Microbiology
BackgroundMetagenomic next-generation sequencing(mNGS) has been widely used in the pathogenetic diagnosis of lower respiratory tract infections. However, the interpretation of pathogens detected by mNGS remains inconclusive.ObjectiveOur study aimed to compare the differential diagnostic value of sequencing reads and the relative abundance of bacteria ...
Duan, Yuanfang, Li, Qin, Fei, Haitao, Song, Jiafu, Xu, Caiyun   +4 more
exaly   +4 more sources

mNGS for identifying pathogens in febrile neutropenic children with hematological diseases [PDF]

International Journal of Infectious Diseases, 2022
To investigate the application value of metagenomic next-generation sequencing (mNGS) in children with hematological diseases presenting with Febrile Neutropenia (FN).We retrospectively analyzed the clinical data of 49 hematological children with FN, and compared the results of mNGS with those of traditional pathogen detection (TPD) and the prognoses ...
Fang Guo
exaly   +4 more sources

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mNGS for the diagnosis of infection in patients with CTDs.

2022
Metagenomic next-generation sequencing (mNGS) is emerging as a promising technique for pathogens detection. Most patients with CTDs under immunosuppression are susceptible to infection. However, reports on the application of mNGS in patients with connective tissue diseases (CTDs) who were suspected infection remain scarce.
openaire   +1 more source