Results 61 to 70 of about 8,440 (225)

Clinical application of amplification-based versus amplification-free metagenomic next-generation sequencing test in infectious diseases

Frontiers in Cellular and Infection Microbiology, 2023
BackgroundRecently, metagenomic next-generation sequencing (mNGS) has been used in the diagnosis of infectious diseases (IDs) as an emerging and powerful tool.
Zhe-Ying Wang, Zhe-Ying Wang, Lu-Lu Li, Xue-Lei Cao, Xue-Lei Cao, Ping Li, Ping Li, Jian Du, Ming-Jin Zou, Li-Li Wang, Li-Li Wang   +10 more
doaj   +1 more source

Engineering and Characterization of a Fluorescent Fission Yeast Arp2/3 Complex for Single Molecule Mechanistic Investigations

Cytoskeleton, EarlyView.
ABSTRACT Arp2/3 complex is a seven‐component protein complex that facilitates the assembly of branched actin filament networks by binding to pre‐existing “mother” actin filaments and initiating the nucleation of new branched “daughter” filaments. Arp2/3 complex must be activated by a nucleation promoting factor such as the WASP/Scar protein family. The
Caitlin A. Anderson, Meghan E. O'Connell, Kaitlin E. Homa, Cristian Suarez, Michael L. James, Kashmeera D. Baboolall, Vilmos Zsolnay, Vladimir Sirotkin, David R. Kovar   +8 more
wiley   +1 more source

A Paired Comparison of Plasma and Bronchoalveolar Lavage Fluid for Metagenomic Next-Generation Sequencing in Critically Ill Patients with Suspected Severe Pneumonia

Infection and Drug Resistance, 2022
Ting Sun,1 Yijie Liu,2 Ying Cai,3 Tianshu Zhai,3 Yun Zhou,4 Bin Yang,5 Xiaojing Wu,3,* Qingyuan Zhan1,3,* 1Capital Medical University China-Japan Friendship School of Clinical Medicine, Beijing, People’s Republic of China; 2Graduate School of ...
Sun T, Liu Y, Cai Y, Zhai T, Zhou Y, Yang B, Wu X, Zhan Q   +7 more
doaj  

Exploring the pathogen diagnosis and prognostic factors of severe COVID-19 using metagenomic next-generation sequencing: A retrospective study [PDF]

Journal of Medical Biochemistry
Background: This study aimed to identify pathogens and factors that predict the outcome of severe COVID-19 by utilizing metagenomic next-generation sequencing (mNGS) technology.
Zeng Weizhong, Liang Yanchao, He Xiaoyuan, Chen Fangwei, Xiong Jiali, Wen Zhenhua, Tang Liang, Chen Xun, Zhang Juan   +8 more
doaj   +1 more source

Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia

Canadian Journal of Infectious Diseases and Medical Microbiology, 2020
Background. Metagenomic next-generation sequencing (mNGS) has made a revolution in the mode of pathogen identification. We decided to explore the diagnostic value of blood and bronchoalveolar lavage fluid (BALF) as mNGS samples in pneumonia. Methods.
Xu Chen, Shuizi Ding, Cheng Lei, Jieli Qin, Ting Guo, Danhui Yang, Min Yang, Jie Qing, Wenlong He, Min Song, Yan Zhang, Huihui Zeng, Qingwu Qin, Lizhen Yang, Yingjiao Long, Yan Chen, Bingyin Ma, Ruoyun Ouyang, Ping Chen, Hong Luo   +19 more
doaj   +1 more source

Comparison of metagenomic next-generation sequencing using cell-free DNA and whole-cell DNA for the diagnoses of pulmonary infections

Frontiers in Cellular and Infection Microbiology, 2022
Although the fast-growing metagenomic next-generation sequencing (mNGS) has been used in diagnosing infectious diseases, low detection rate of mNGS in detecting pathogens with low loads limits its extensive application.
Ping He, Jing Wang, Rui Ke, Wei Zhang, Pu Ning, Dexin Zhang, Xia Yang, Hongyang Shi, Ping Fang, Zongjuan Ming, Wei Li, Jie Zhang, Xilin Dong, Yun Liu, Jiemin Zhou, Han Xia, Shuanying Yang   +16 more
doaj   +1 more source

Prevalence of Colonization, Association With Disease Stage and Effect of Eradication of Staphylococcus aureus in Cutaneous T‐Cell Lymphoma: A Systematic Review and Meta‐Analysis

JEADV Clinical Practice, EarlyView.
ABSTRACT Background Cutaneous T‐cell lymphoma (CTCL) is a rare and heterogeneous group of skin‐homing lymphomas with a largely unknown pathogenesis. Staphylococcus aureus (S. aureus) colonization has been implicated in exacerbating CTCL, particularly in advanced stages. Objectives This study assessed the prevalence of S.
Ida Lind Holm, Sarah Søltoft Rasmussen, Somaia Naassan, Misbah N. Ghazanfar, Kenneth Thomsen, Rikke Bech, Lise M. Lindahl, Maria Kamstrup, Simon Francis Thomsen, Zarqa Ali   +9 more
wiley   +1 more source

mNGS-based dynamic pathogen monitoring for accurate diagnosis and treatment of severe pneumonia caused by fungal infections [PDF]

, 2023
Metagenomic next-generation sequencing (mNGS) has been widely applied to identify pathogens associated with infectious diseases. However, limited studies have explored the use of mNGS-based dynamic pathogen monitoring in intensive care unit patients with
Wenjie Tan, Li-An Tang, Changcheng Wu, Wenling Wang, Yinjie Liang, Chuanyi Ning, Zhen Li, Ruhan A, Debin Huang   +8 more
core   +1 more source

Clinical pathogen profiles and lung microbiome features in lung infection patients and concurrent cancer: insights from metagenomics next-generation sequencing

Open Life Sciences
Pulmonary infections in immunocompromised cancer patients present significant diagnostic and therapeutic challenges. From Dec 2021 to Aug 2023, 85 patients with pulmonary infection were enrolled and categorized into a cancer group (CP, n = 20) and a non ...
Liu Dandan, Ma Yuehong, Ma Qian, Huang Huihua, Li Tianyu, Wang Jin, Zhang Jin, Cheng Xuesong, Ge Xueru, Chen Yan, Zhang Yanbei   +10 more
doaj   +1 more source

The Performance of Metagenomic Next-Generation Sequence in the Diagnosis of Suspected Opportunistic Infections in Patients with Acquired Immunodeficiency Syndrome [Corrigendum]

Infection and Drug Resistance, 2023
Liu L, Yuan M, Sun S, et al. Infect Drug Resist. 2022;15:5645–5653. The authors have advised that affiliation 1 on page 5645 is incorrect.
Liu L, Yuan M, Sun S, Wang J, Shi Y, Yu Y, Su X   +6 more
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