Results 71 to 80 of about 680,200 (262)

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Seamless Function-Oriented Mechanical System Architectures and Models

Eng
One major challenge of today’s product development is to master the constantly increasing product complexity driven by the interactions between different disciplines, like mechanical, electrical and software engineering.
Christian Wyrwich, Kathrin Boelsen, Georg Jacobs, Thilo Zerwas, Gregor Höpfner, Christian Konrad, Joerg Berroth   +6 more
doaj   +1 more source

Organizational Simulation for Model Based Systems Engineering

Procedia Computer Science, 2013
AbstractOrganizations creating complex systems often have hierarchical team networks; this structure affects system performance because some teams have greater influence over dimensions of parts produced by other teams. Dimensional interdependencies among components produce ripple-effects; examples include load paths and thermal flows.
Daniel A. O'Neil, Mikel D. Petty
openaire   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Engineering Systems with Standards and Digital Models: Specifying Stakeholder Needs and Capabilities—MGOS

Systems
This paper proposes a formal method and associated techniques for completing the ISO/IEC/IEEE Standard 15288 technical process 6.4.2—Stakeholder Needs and Requirements definition within the 15288-SysML Grid framework.
Kevin MacG. Adams, Irfan Ibrahim, Steven L. Krahn   +2 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Model Based Systems Engineering for Smart Grids as Systems of Systems

Procedia Computer Science, 2011
AbstractThis paper presents a System of Systems (SOS) approach to understand, analyze, and design modern energy Smart Grids using Model-Based Systems Engineering (MBSE) methodologies. The concept and definition of a Smart Grid as SOS is presented and Smart Grids are analyzed in the form of detailed architectural artifacts with System Modeling Language (
Amit J. Lopes, R. Lezama, Ricardo Pineda
openaire   +1 more source

Phosphoinositides and inositol phosphates as molecular glues

FEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry, Alexander J. Cutright, Mong Na Claire Loi, Jessica Martin, Joseph W. Gilligan, Jakub N. Kubina, Michael J. Arambula, Susanna Tanada Palmu, Daniella Zhou, Raymond D. Blind   +9 more
wiley   +1 more source