Results 101 to 110 of about 1,433,700 (300)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Preclinical pharmacology characterization of HX009, a novel PD1 x CD47 Bi-specific antibody

Scientific Reports
Certain immune-checkpoint inhibitors have a narrow therapeutic window (TW) as cancer therapeutics, and engineered dual-/multi-targeting agents could potentially widen the TW to bring true clinical benefits.
Hang Ke, Tao Yang, Faming Zhang, Cen Chen, Jingjing Wang, Jinping Liu, Xiaoyu An, Lingxin Xiong, Xianfei He, Lei Zhang, Qi-Xiang Li   +10 more
doaj   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Invertebrate and vertebrate models in aging research

Biomedical Papers, 2019
Therapeutic interventions that can delay age associated diseases and ensure a longer health-span is a major goal of aging research. Consequent to understanding that aging is a modifiable trait, a large number of studies are currently being undertaken to ...
Manoj Kumar Chaudhary, Syed Ibrahim Rizvi   +1 more
doaj   +1 more source

A MULTI-ATTRIBUTE MODEL OF PUBLIC ACCEPTANCE OF GENETICALLY MODIFIED ORGANISMS [PDF]

Using Fishbein's multiattribute model as a theoretical background, this paper develops an empirical model to assess and identify attributes of agrobiotechnology and individual characteristics determining public acceptance of biotech foods.
Balasubramanian, Siva K., Moon, Wanki
core   +1 more source

ANN for Predicting Antibiotic Susceptibility [PDF]

, 2016
: In this research, an Artificial Neural Network (ANN) model was developed and tested to predict efficiency of antibiotics in treating various bacteria types. Attributes that were taken in account are: organism name, specimen type, and antibiotic name as
Ahmed, Maaruf, Randa, Qassas
core  

Asexual and sexual replication in sporulating organisms

, 2006
This paper develops models describing asexual and sexual replication in sporulating organisms. Replication via sporulation is the replication strategy for all multicellular life, and may even be observed in unicellular life (such as with budding yeast ...
Bohyun Lee, C. O. Wilke, C. S. Potten, D. B. Dusenbery, Emmanuel Tannenbaum, G. Bell, G. C. Williams, H. J. Muller, J. Felsenstein, J. Haigh, J. Maynard-Smith, J. R. Merok, K. Negishi, L. Kelly, P. Schuster, R. E. Michod, W. Ebeling   +16 more
core   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source