Results 61 to 70 of about 1,154,864 (307)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Synergy between explicit teaching, ICT integration, and the TaRL approach: structural modeling of learning gains

Frontiers in Education
This study examines the effectiveness of the Teaching at the Right Level (TaRL) program when enriched by the judicious use of Information and Communication Technologies for Education (ICTE), from an explicit teaching perspective.
Mounia Machkour, Hiba Gharbaoui, Latifa Lamalif, Sophia Faris, Khalifa Mansouri   +4 more
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Mathematical modelling and control approach for sustainable ecosystems in mitigating the impact of pollutants on aquatic species in rivers

Scientific Reports
This paper focuses on the urgent issue of minimising the impact of pollutants on aquatic life in river ecosystems. Our innovative approach involves the integration of mathematical modelling and strategic control methods to counteract the negative ...
Marouane Karim, Imane Dehaj, Ali Akgül, Murad Khan Hassani, Hanane Ferjouchia, Mostafa Rachik   +5 more
doaj   +1 more source

Coupled thermal and mechanical analysis of composite cross sections using mathematical optimization strategies

Engineering Structures and Technologies, 2017
In the present article an alternative approach for the coupled thermal and mechanical analysis of composite cross sections under temperature effects is introduced, which uses the mathematical optimization as a consistent methodical base.
Christopher Taube, Hans-Georg Timmler, Marcel Helmrich, Guido Morgenthal   +3 more
doaj   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

A New Paradigm for Scientific Computing: Accelerated Algorithm Development With Large Reasoning Models

IEEE Access
This study examines the application of Large Reasoning Model (LRM)-based artificial intelligence (AI) agents to accelerate scientific discovery, with a specific focus on the rapid prototyping of numerical algorithms.
Ioannis W. Kokkinakis, Dimitris Drikakis
doaj   +1 more source

Shock Reduction through Opposing Jets—Aerodynamic Performance and Flight Stability Perspectives

Applied Sciences, 2019
In this research paper, investigations of counter flow (opposing) jet on the aerodynamic performance, and flight stability characteristics of an airfoil with blunt leading-edge in supersonic regime are performed. Unsteady Reynolds-Averaged Navier-Stokes (
Shagufta Rashid, Fahad Nawaz, Adnan Maqsood, Rizwan Riaz, Shuaib Salamat   +4 more
doaj   +1 more source