Results 171 to 180 of about 37,287 (222)

DNAzyme amplification of molecular beacon signal

Talanta, 2005
This paper reports an improved catalytic molecular beacon. Addition of the target oligonucleotide activates a DNA enzyme (DNAzyme), which, in turn, activates multiple copies of molecular beacons (MB) and gives rise to a strong fluorescence signal.
Chengde Mao
exaly   +3 more sources

Oligothiophene Molecular Beacons [PDF]

Bioconjugate Chemistry, 2007
Oligomers of thiophene are widely studied compounds for their electronic and optoelectronic properties. Despite their strong fluorescence, their use as markers for biomolecules, especially for oligonucleotides (ONs), is still largely unexplored. Here, we describe the synthesis of a series of ON molecular beacons employing different oligothiophenes as ...
A Cazzato, M L Capobianco, M Zambianchi, L Favaretto, C Bettini, G Barbarella   +5 more
openaire   +3 more sources

Catalytic Molecular Beacons

ChemBioChem, 2001
We have constructed catalytic molecular beacons from a hammerhead-type deoxyribozyme by a modular design. The deoxyribozyme was engineered to contain a molecular beacon stem-loop module that, when closed, inhibits the deoxyribozyme module and is complementary to a target oligonucleotide. Binding of target oligonucleotides opens the beacon stem-loop and
M N, Stojanovic, P, de Prada, D W, Landry   +2 more
openaire   +2 more sources

Molecular beacons for bioanalytical applications

The Analyst, 2005
Molecular beacons (MBs) are hairpin-shaped oligonucleotides that contain both fluorophore and quencher moieties. They act like switches and are normally in a closed state, when the fluorophore and the quencher are brought together to turn "off" the fluorescence.
Li, Tan, Ying, Li, Timothy J, Drake, Leonid, Moroz, Kemin, Wang, Jun, Li, Alina, Munteanu, James Yang, Chaoyong, Karen, Martinez, Weihong, Tan   +9 more
openaire   +2 more sources

Wavelength-shifting molecular beacons

Nature Biotechnology, 2000
We describe wavelength-shifting molecular beacons, which are nucleic acid hybridization probes that fluoresce in a variety of different colors, yet are excited by a common monochromatic light source. The twin functions of absorption of energy from the excitation light and emission of that energy in the form of fluorescent light are assigned to two ...
S, Tyagi, S A, Marras, F R, Kramer
openaire   +2 more sources

Polyvalent Carbocyanine Molecular Beacons for Molecular Recognitions

Journal of the American Chemical Society, 2004
Polyvalent carboxylate-terminating near-infrared (NIR) carbocyanine molecular beacons were prepared by homologation of reactive carboxyl groups of the beacon with imino diacetic acid. Their conjugation with unprotected d-(+)-glucosamine gave dendritic arrays of the carbohydrate on an inner NIR chromophore core.
Yunpeng, Ye, Sharon, Bloch, Samuel, Achilefu   +2 more
openaire   +2 more sources

Activation Kinetics of Zipper Molecular Beacons

The Journal of Physical Chemistry B, 2014
Proteases play key roles in the regulation of normal cellular function, and thus, their deregulation leads to many disease states. Molecular beacons are promising protease-imaging probes for the detection and characterization of disease as well as for the evaluation of treatment. Inspired by this, we examined the efficiency of zipper molecular beacons (
Tracy W, Liu, Juan, Chen, Laura, Burgess, Brian C, Wilson, Gang, Zheng, Lixin, Zhan, Wing-Ki, Liu, Bae-Yeun, Ha   +7 more
openaire   +2 more sources

Locked Nucleic Acid Molecular Beacons

Journal of the American Chemical Society, 2005
A novel LNA-MB (molecular beacon based on locked nucleic acid bases) has been designed and investigated. It exhibits very high melting temperature and is thermally stable, shows superior single base mismatch discrimination capability, and is stable against digestion by nuclease and has no binding with single-stranded DNA binding proteins.
Lin, Wang, Chaoyong James, Yang, Colin D, Medley, Steven A, Benner, Weihong, Tan   +4 more
openaire   +2 more sources

Multicolor molecular beacons for allele discrimination

Nature Biotechnology, 1998
Molecular beacons are hairpin-shaped oligonucleotide probes that report the presence of specific nucleic acids in homogenous solutions. When they bind to their targets they undergo a conformational reorganization that restores the fluorescence of an internally quenched fluorophore.
S, Tyagi, D P, Bratu, F R, Kramer
openaire   +2 more sources

Molecular Aptamer Beacons

2013
In this chapter, we present a special class of nucleic acid probes, called molecular aptamer beacons, which combine the specific recognition of aptamers and the signal transduction of molecular beacons. By using SELEX process, single-stranded aptamers can be selected to bind essentially any targets ranging from ions, small molecules, peptides, proteins,
Cuichen Wu, Chaoyong James Yang, Weihong Tan   +2 more
openaire   +1 more source