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Biosensors based on molecular beacons
Chemical Papers, 2015AbstractA fundamental molecular beacon (MB) consists of a special short nucleic acid strand with a fluorophore-quencher pair attached to its ends. It provides a unique framework that is susceptible to conformational transitions between a hairpin (closed) conformation and an extended (open) conformation.
Magdalena Stobiecka, Agata Chałupa
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2009
Molecular beacons are single-stranded oligonucleotide hybridization probes with a stem-and-loop structure that recognize and report the presence of specific nucleic acids in homogeneous solutions (Tyagi et al. 1996). The loop contains a probe sequence that is complementary to a target sequence, and the stem is formed by the annealing of complementary ...
Zhiguo Wang, Baofeng Yang
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Molecular beacons are single-stranded oligonucleotide hybridization probes with a stem-and-loop structure that recognize and report the presence of specific nucleic acids in homogeneous solutions (Tyagi et al. 1996). The loop contains a probe sequence that is complementary to a target sequence, and the stem is formed by the annealing of complementary ...
Zhiguo Wang, Baofeng Yang
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Measurement of molecular beacons in the SPECTRAmax� GEMINI spectrofluorometer
Journal of Chemical Technology & Biotechnology, 2000This article discusses the use of a dual monochromator based microplate spectrofluorometer in the analysis of molecular beacons used to monitor PCR reactions. The SPECTRAmax GEMINI spectrofluorometer is the first such instrument that incorporates Excitation and Emission dual scanning monochromators in its design. This feature gives the sensitivity that
R P Dennis, E McGown, M Su
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Molecular Beacons: Probes that Fluoresce upon Hybridization
Nature Biotechnology, 1996We have developed novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions. These probes undergo a spontaneous fluorogenic conformational change when they hybridize to their targets. Only perfectly complementary targets elicit this response, as hybridization does not occur when the target ...
S, Tyagi, F R, Kramer
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Recent trends in molecular beacon design and applications
Analytical and Bioanalytical Chemistry, 2011A molecular beacon (MB) is a hairpin-structured oligonucleotide probe containing a photoluminescent species (PLS) and a quencher at different ends of the strand. In a recognition and detection process, the hybridization of MBs with target DNA sequences restores the strong photoluminescence, which is quenched before hybridization.
Kewei, Huang, Angel A, Martí
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Programmed Nanoparticle Aggregation Using Molecular Beacons
Angewandte Chemie, 2010Nucleic acids can be used as robust building blocks or scaffolds for new nanoarchitectures. The potential of nucleic acids has been demonstrated with the DNA-based construction of a variety of shapes including, for example, an incredible range of two-dimensional patterns, three-dimensional crystals, and even containers with lids that can be opened. DNA
Jonathan F, Lovell +3 more
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2013
During the detection in complex biological system, similar to other fluorescence probes, the molecular beacons suffer severely from the background signal interference. Recent studies indicated that excimer molecular beacon (EMB) can address the problem.
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During the detection in complex biological system, similar to other fluorescence probes, the molecular beacons suffer severely from the background signal interference. Recent studies indicated that excimer molecular beacon (EMB) can address the problem.
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Molecular Beacons for Protein—DNA Interaction Studies
2008Real-time monitoring of DNA-protein interactions involving molecular beacon (MB) and molecular beacon aptamer (MBA) was discussed in this chapter. MBs are single-stranded oligonucleotide probes with a hairpin structure. MBs have been designed for oligonucleotide recognition and protein-DNA interaction studies.
Jun, Li +4 more
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Molecular Beacons on Solid Surfaces
2013In this chapter, immobilization of molecular beacons (MBs) on different bulk surfaces and nanomaterial surfaces is discussed. For use in large-scale parallel analysis with increased sensitivity at a pathogen-detector interface, MBs have been immobilized on different bulk solid support matrixes, including microscope glass slides, gold surfaces, and ...
Tao Chen, Weihong Tan
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Molecular beacon: a multitask probe
Journal of Applied Microbiology, 2005G, Goel +4 more
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