Results 71 to 80 of about 315,729 (282)
Engineered gene networks enable non-genetic drug resistance and enhanced cellular robustness
Engineering Biology, 2019 Drug resistance complicates the treatment of cancer and infectious diseases, and often arises from the elevated expression of a gene that neutralises or reduces drug activity.
Brendan Camellato +6 more
doaj +1 more source
Structural basis for the inhibition of RecBCD by Gam and its synergistic antibacterial effect with quinolones [PDF]
, 2016 Our previous paper (Wilkinson et al, 2016) used high-resolution cryo-electron microscopy to solve the structure of the Escherichia coli RecBCD complex, which acts in both the repair of double-stranded DNA breaks and the degradation of bacteriophage DNA ...
Aedo +44 more
core +5 more sources
FEBS Open Bio, EarlyView.Tandem VHH targeting distinct EGFR epitopes were engineered into a monovalent bispecific antibody (7D12‐EGA1‐Fc) with more potent ADCC without increasing affinity to EGFR. Structural modeling of 7D12‐EGA1‐Fc showed cross‐linking of separate EGFR domains to enhance CD16a engagement on NK cells.
Yuqiang Xu +5 more
wiley +1 more source
Machine learning-driven protein engineering: a case study in computational drug discovery
Engineering Biology, 2020 Research and development in drug discovery will need to find significant efficiency gains if the industry is to continue generating novel drugs. There is great expectation for machine learning (ML) to provide this boost in R&D productivity, but to ...
Harry F. Rickerby +2 more
doaj +1 more source
Marriages of Mathematics and Physics: A Challenge for Biology [PDF]
, 2017 The human attempts to access, measure and organize physical phenomena have led to a manifold construction of mathematical and physical spaces. We will survey the evolution of geometries from Euclid to the Algebraic Geometry of the 20th century.
Islami, Arezoo, Longo, Giuseppe
core +4 more sources
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
STAR ProtocolsSummary: Here, we present a protocol for encapsulating DNA molecules under crowded conditions within cell-sized lipid-coated droplets. We describe steps for preparing a lipid-oil mixture and adding an aqueous solution containing DNA, which, when mixed ...
Mehdi Shafiei Aporvari +2 more
doaj +1 more source
Large-scale-free network organisation is likely key for biofilm phase transition
Engineering Biology, 2019 Non-linear Kuramoto model has been used to study synchronised or sync behaviour in numerous fields; however, its application in biology is scarce. Here, the basic model has been introduced and examples where large-scale small-world or scale-free networks
Kumar Selvarajoo
doaj +1 more source
Evidence for DNA-mediated nuclear compartmentalization distinct from phase separation. [PDF]
, 2019 RNA Polymerase II (Pol II) and transcription factors form concentrated hubs in cells via multivalent protein-protein interactions, often mediated by proteins with intrinsically disordered regions.
Darzacq, Xavier +9 more
core
Inhibition by small-molecule ligands of formation of amyloid fibrils of an immunoglobulin light chain variable domain. [PDF]
, 2015 Overproduction of immunoglobulin light chains leads to systemic amyloidosis, a lethal disease characterized by the formation of amyloid fibrils in patients' tissues. Excess light chains are in equilibrium between dimers and less stable monomers which can
Brumshtein, Boris +7 more
core +1 more source