Results 71 to 80 of about 318,972 (299)
Mechanistic modelling of a recombinase-based two-input temporal logic gate
Engineering Biology, 2017 Site-specific recombinases (SSRs) mediate efficient manipulation of DNA sequences in vitro and in vivo. In particular, serine integrases have been identified as highly effective tools for facilitating DNA inversion, enabling the design of genetic ...
Jack E. Bowyer +3 more
doaj +1 more source
Structure-based inhibitors of amyloid beta core suggest a common interface with tau. [PDF]
, 2019 Alzheimer's disease (AD) pathology is characterized by plaques of amyloid beta (Aβ) and neurofibrillary tangles of tau. Aβ aggregation is thought to occur at early stages of the disease, and ultimately gives way to the formation of tau tangles which ...
Bowler, Jeannette +13 more
core +1 more source
The acidic domain of the endothelial membrane protein GPIHBP1 stabilizes lipoprotein lipase activity by preventing unfolding of its catalytic domain. [PDF]
, 2016 GPIHBP1 is a glycolipid-anchored membrane protein of capillary endothelial cells that binds lipoprotein lipase (LPL) within the interstitial space and shuttles it to the capillary lumen.
Beigneux, Anne P +9 more
core +4 more sources
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
N-terminal tagging of RNA Polymerase II shapes transcriptomes more than C-terminal alterations
iScienceSummary: RNA polymerase II (Pol II) has a C-terminal domain (CTD) that is unstructured, consisting of a large number of heptad repeats, and whose precise function remains unclear.
Adam Callan-Sidat +4 more
doaj +1 more source
Thermofluidic chip containing virtual thermal wells
Engineering Biology, 2019 The authors describe a thermofluidic chip on which microscale islands of controlled temperature are formed within an open fluidic environment. The chip forms part of the authors’ technology for thermally controlled DNA synthesis, whereby the site ...
Andrew J. Ferguson +6 more
doaj +1 more source
Engineering Biology, 2020 Duchenne muscular dystrophy (DMD) is an X-linked genetic disease affecting 1 in 5000 young males worldwide annually. Patients experience muscle weakness and loss of ambulation at an early age, with ∼75% reduced life expectancy. Recently developed genetic
Marthe Helene Solberg +2 more
doaj +1 more source
FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
Molecular and Cellular Biophysics [PDF]
Choice Reviews Online, 2006 Molecular and Cellular Biophysics provides advanced undergraduate and graduate students with a foundation in the basic concepts of biophysics. Students who have taken physical chemistry and calculus courses will find this book an accessible and valuable aid in learning how these concepts can be used in biological research.
openaire +2 more sources
Data model for biopart datasheets
Engineering Biology, 2018 This study introduces a new data model, based on the DICOM-SB (see glossary of terms for definition of acronyms) standard for synthetic biology, that is capable of describing/incorporating the data, metadata and ancillary information from detailed ...
Iñaki Sainz de Murieta +2 more
doaj +1 more source