Results 71 to 80 of about 191,889 (301)
FEBS Letters, EarlyView.The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson +6 more
wiley +1 more source
Biophysics: An Introduction [PDF]
, 2012 Biophysics is the science of physical principles underlying all processes of life, including the dynamics and kinetics of biological systems. This fully revised 2nd English edition is an introductory text that spans all steps of biological organization ...
Glaser, Roland
core +1 more source
Molecular Mechanism of the Anti-Inflammatory Action of Heparin [PDF]
, 2021 Our objective is to reveal the molecular mechanism of the anti-inflammatory action of low-molecular-weight heparin (LMWH) based on its influence on the activity of two key cytokines, IFNγ and IL-6.
Peicho Petkov +11 more
core +1 more source
Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes
FEBS Letters, EarlyView.Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez +2 more
wiley +1 more source
High speed multispectral fluorescence lifetime imaging [PDF]
, 2013 We report a spectrally resolved fluorescence lifetime imaging system based on time gated single photon detection with a fixed gate width of 200 ps and 7 spectral channels.
Sub Soft Condensed Matter +5 more
core +1 more source
Combined Effects of Methylated Cytosine and Molecular Crowding on the Thermodynamic Stability of DNA Duplexes [PDF]
, 2021 Methylated cytosine within CpG dinucleotides is a key factor for epigenetic gene regulation. It has been revealed that methylated cytosine decreases DNA backbone flexibility and increases the thermal stability of DNA.
Daisuke Miyoshi +3 more
core +1 more source
Data-driven enzyme immobilisation: a case study using DNA to immobilise galactose oxidase
Engineering Biology, 2020 Biocatalysis has the potential to enable green chemistry. New methods of enzyme immobilisation will be required to improve enzyme stability, product purification, and compatibility of different enzymes in the same reaction conditions.
Wolfgang Ott +4 more
doaj +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Large Multidomain Protein NMR: HIV-1 Reverse Transcriptase Precursor in Solution [PDF]
, 2020 NMR studies of large proteins, over 100 kDa, in solution are technically challenging and, therefore, of considerable interest in the biophysics field.
Zhaoyong Xi +4 more
core +1 more source
Structural and Kinetic Views of Molecular Chaperones in Multidomain Protein Folding [PDF]
, 2022 Despite recent developments in protein structure prediction, the process of the structure formation, folding, remains poorly understood. Notably, folding of multidomain proteins, which involves multiple steps of segmental folding, is one of the biggest ...
Soichiro Kawagoe +2 more
core +1 more source