Results 141 to 150 of about 472,091 (296)

Molecular cloning and characterization of a human eotaxin receptor expressed selectively on eosinophils. [PDF]

, 1996
Paul Ponath, Suofu Qin, T W Post, Jian-Feng Wang, Lin Wu, N P Gerard, Walter Newman, Craig Gerard, Charles R. Mackay   +8 more
openalex   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Molecular Cloning, Bioinformatics, and Expression Analysis of the <i>NPR1</i> Homolog in Sesame (<i>Sesamum indicum</i> L.). [PDF]

Plants (Basel)
Yan M, Zhao X, Li X, He Z, Hua J, Wei L, Sun Y, Wan C, Huang S.   +8 more
europepmc   +1 more source

Molecular Cloning and Functional Characterization of the 5' Regulatory Region of the SLC11A1 Gene from Yaks. [PDF]

Animals (Basel), 2023
Chong Y, Wang L, Wang B, Gao Z, Lu Y, Deng W, Xi D.   +6 more
europepmc   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Molecular cloning and the expression profile of two calnexin genes - CNX1 and CNX2 - during pollen development and pollen tube growth in Petunia. [PDF]

BMC Plant Biol
Suwińska A, Wasąg P, Lenartowska M, Tyburski J, Lenartowski R.   +4 more
europepmc   +1 more source

Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276

FEBS Open Bio, EarlyView.
Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig, Swanhild Lohse, Sara Elahi, Nils Hartmann, Stefanie Deckert, Shutian Si, Alexander Desuki, Anja Harder   +7 more
wiley   +1 more source

Single genome amplification and molecular cloning of HIV-1 populations in acute HIV-1 infection: implications for studies on HIV-1 diversity and evolutionary rate. [PDF]

Virus Evol
Hsieh AYY, Hassan AS, Nazziwa J, Lindquist L, Karlson S, Hare J, Kamali A, Karita E, Kilembe W, Price MA, Björkman P, Kaleebu P, Allen S, Hunter E, Gilmour J, Rowland-Jones SL, Sanders EJ, Esbjörnsson J.   +17 more
europepmc   +1 more source

ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

FEBS Open Bio, EarlyView.
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa Takano   +8 more
wiley   +1 more source

Molecular Cloning and Characterization of Estrogen-Related Receptor Gene in <i>Corbicula fluminea</i>: Expression Profiles in Response to Bisphenol A and Its Substitutes Exposure. [PDF]

Biology (Basel)
Xu R, Guo W, Zhang P, Zhang C.
europepmc   +1 more source