Results 311 to 320 of about 4,711,930 (381)
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Fish & Shellfish Immunology, 2020
Transcription factor c-Jun is a member of AP-1 transcription complex that can be induced by various pathogens and plays a broad regulatory role in vertebrate immune response. In this study, the complete c-Jun cDNA of large yellow croaker Larimichthys crocea (Lcc-Jun) was cloned, whose open reading frame (ORF) is 984 bp long and encodes a protein of 327
Yuhong Chen +5 more
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Transcription factor c-Jun is a member of AP-1 transcription complex that can be induced by various pathogens and plays a broad regulatory role in vertebrate immune response. In this study, the complete c-Jun cDNA of large yellow croaker Larimichthys crocea (Lcc-Jun) was cloned, whose open reading frame (ORF) is 984 bp long and encodes a protein of 327
Yuhong Chen +5 more
openaire +4 more sources
Methods in Enzymology, 2013
This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
J. Lessard
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This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
J. Lessard
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2020
A variety of strategies have been used to obtain cDNA and genomic clones encoding ricin. Since their isolation these sequences have been manipulated to allow expression of A chain (19) and A chain mutants (15,20,34), B chain (14,21-23) and proricin (24).
L M, Roberts, J W, Tregear, J M, Lord
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A variety of strategies have been used to obtain cDNA and genomic clones encoding ricin. Since their isolation these sequences have been manipulated to allow expression of A chain (19) and A chain mutants (15,20,34), B chain (14,21-23) and proricin (24).
L M, Roberts, J W, Tregear, J M, Lord
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Molecular cloning of human RP105
European Journal of Immunology, 1997AbstractRP105 is a 105‐kDa type I membrane protein of the leucine‐rich repeat (LRR) family. Anti‐RP105 sensitizes B cells to antigen‐receptor‐mediated apoptosis, but protects B cells from radiation‐induced apoptosis and stimulates B cell proliferation. The sequence of the mouse RP105 has been reported.
Odette de Bouteiller +9 more
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Molecular cloning of the cDNA for ligatin
Journal of Cell Science, 1989ABSTRACT We describe the first isolation and sequence of a partial cDNA clone encoding ligatin, a trafficking receptor for phosphoglycoproteins. The clone was isolated from a human U937 promonocyte λgtll cDNA library using rabbit antiserum to rat ileal ligatin.
Ye-Shih Ho +3 more
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Molecular Cloning in the Streptococci
1982The genus Streptococcus contains a large number of species able to cause infection in humans and in animals. Our research efforts have revolved around the study of streptococci that normally reside in the human oral cavity. In this regard, Streptococcus mutans is thought to play an important role in the etiology of dental caries (tooth decay) in humans
Francis L. Macrina +3 more
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Molecular Cloning of PCR Products
Current Protocols in Molecular Biology, 2001AbstractIt is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high‐quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified
Ayoub Rashtchian +2 more
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Molecular Cloning of Steroid Hydroxylases
Endocrine Research, 1984Recombinant plasmids specific for bovine adrenocortical cytochromes P-450scc and P-45011 beta have been identified and characterized. Using these cDNA inserts as probes, it is found that tissue specificity of gene expression for these two proteins is as expected. Cytochrome P-450scc mRNA is found in adrenocortical and corpus luteum RNA while cytochrome
Evan R. Simpson +3 more
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[26] Molecular cloning of laminin
1987Publisher Summary Molecular cloning can be used to answer questions about the domain structure of laminin, its relationship to other matrix glycoproteins, the subunit composition of the laminin synthesized by different cell types, the possible existence of alternate forms of laminin polypeptides, the structure and chromosomal localization of laminin ...
Barlow, DP, McVey, JH, Hogan, BLM
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Molecular cloning of feline CD34
Veterinary Immunology and Immunopathology, 2003In humans, baboons, dogs and mice CD34 is a cell surface molecule that is expressed on primitive hematopoietic cells and in all these species CD34 positive cells can be used to effect long-term haematopoietic reconstitution. CD34 positive haematopoietic cells therefore provide a convenient and relatively small cell population to target when attempting ...
Gouri Yogalingam +2 more
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