Results 321 to 330 of about 4,711,930 (381)

Molecular cloning in heterologous systems

1983
During the last years the knowledge accumulated by fundamental research of DNA recombination in vitro became more relevant to biotechnology.
Christine Dr. Lang-Hinrichs, Karl Esser
openaire   +3 more sources

Molecular Cloning of Human Amidophosphoribosyltransferase

Biochemical and Biophysical Research Communications, 1993
The cDNA of human amidophosphoribosyltransferase (EC 2.4.2.14, ATase), which is the supposed regulatory allosteric enzyme of de novo purine nucleotide biosynthesis, has been cloned from human hepatoma (HepG2) cDNA library. The predicted open reading frame encodes a protein of 517 amino acids with a deduced molecular weight (Mr) of 57,398, which is ...
H, Iwahana, J, Oka, N, Mizusawa, E, Kudo, S, Ii, K, Yoshimoto, E W, Holmes, M, Itakura   +7 more
openaire   +3 more sources

Molecular Cloning of the Thyrotropin Receptor

Science, 1989
The pituitary hormone thyrotropin, or thyroid-stimulating hormone (TSH), is the main physiological agent that regulates the thyroid gland. The thyrotropin receptor (TSHR) was cloned by selective amplification with the polymerase chain reaction of DNA segments presenting sequence similarity with genes for G protein-coupled receptors. Out of
Parmentier, Marc, Libert, Frédérick, Maenhaut, Carine, Lefort, Anne, Gérard, Claude, Perret, Jason, Van Sande, Jacqueline, Dumont, Jacques, Vassart, Gilbert   +8 more
openaire   +3 more sources

Molecular Cloning of HumanFrizzled-6,

Biochemical and Biophysical Research Communications, 1998
The Frizzled genes encode receptors for WNTs, secreted glycoproteins implicated in development as well as in carcinogenesis. In this paper, we report molecular cloning of Hfz6, the human homologue of Mfz6. Nucleotide sequence analysis showed that the Hfz6 gene encodes the 706 amino-acid protein with seven transmembrane domains, a cystein-rich domain in
Masaru Katoh, Yutaka Atomi, Masaaki Terada, Momoki Hirai, Makoto Tokuhara   +4 more
openaire   +3 more sources

Isolation and molecular cloning of a fish myeloperoxidase

Molecular Immunology, 2008
Myeloperoxidase (MPO) is a conspicuous enzyme in neutrophils of many fish species. Although the MPO gene has been identified in some fish species, the structure and functions of the protein remain to be determined in these vertebrates. In the present study, we isolated turbot neutrophil MPO from kidney cells by affinity chromatography, with Ulva rigida
Castro, Rosario, Piazzon de Haro, María Carla, Noia, Manuel, Leiro, José M., Lamas, Jesús   +4 more
openaire   +4 more sources

Molecular cloning of the interleukin-1 beta converting enzyme.

Science, 1992
Interleukin-1 beta (IL-1 beta) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor.
D. Cerretti, C. Kozlosky, B. Mosley, N. Nelson, K. V. Ness, T. Greenstreet, C. March, Kronheim, T. Druck, LA Cannizzaro   +9 more
semanticscholar   +1 more source

Molecular cloning and expression of internalin in Listeria

1994
Publisher Summary This chapter discusses the molecular cloning and expression of internalin in Listeria . Listeria monocytogenes is a ubiquitous gram-positive bacterium that causes severe infections in humans and in a variety of warm-blooded animals. This pathogen has emerged as an important agent of foodborne disease in the last decade.
Gaillard, Jean-Louis, Dramsi, Shaynoor, Berche, Patrick, Cossart, Pascale   +3 more
openaire   +3 more sources

Molecular cloning of mouse allantoicase cDNA

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2001
The uric acid degradation pathway is progressively lost during vertebrate evolution. In mammals, the end product of this catabolic pathway is allantoin and, therefore, no allantoicase should be present in mouse tissues. Surprisingly, we have found an expressed sequence tag (EST) from mouse testis with high similarity to allantoicase.
VIGETTI, DAVIDE, MONETTI C, BERNARDINI, GIOVANNI BATTISTA   +2 more
openaire   +4 more sources

Molecular cloning of lupin leghemoglobin cDNA

Molecular Biology Reports, 1987
Poly(A)+ RNA isolated from root nodules of yellow lupin (Lupinus luteus, var. Ventus) has been used as a template for the construction of a cDNA library. The ds cDNA was synthesized and inserted into the Hind III site of plasmid pBR 322 using synthetic Hind III linkers.
Konieczny, A, Jensen, E O, Marcker, K A, Legocki, A   +3 more
openaire   +4 more sources

Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.

Science, 1985
Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce
G. Wong, JS Witek, P. Temple, KM Wilkens, AC Leary, DP Luxenberg, Jones Ss, E. Brown, Kay Rm, Orr Ec   +9 more
semanticscholar   +1 more source