Results 1 to 10 of about 2,165,084 (288)
eLife, 2022 The rapid development of new imaging approaches is generating larger and more complex datasets, revealing the time evolution of individual cells and biomolecules. Single-molecule techniques, in particular, provide access to rare intermediates in complex,
Nadia M Huisjes +7 more
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Nature Communications, 2022 TRIM E3 ligases are key regulators of numerous cellular functions, yet their mechanism of action remains poorly understood. Here, the authors show that paralogous TRIM2 and TRIM3 adopt different self-association and activity profiles but interact in ...
Diego Esposito +3 more
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Biotechnology & Biotechnological Equipment, 2021 In the recent years, the application of new antitumor drugs has focused on the replacement of conventional chemotherapeutics with compounds derived from natural products.
Jordana Todorova +4 more
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Benchmarking tomographic acquisition schemes for high-resolution structural biology
Nature Communications, 2020 Here the authors systematically benchmark cryo-electron tomography acquisition schemes to optimize the attainable resolution for subtomogram averaging, and find that dose-symmetric acquisition with even angular sampling provides a better outcome than ...
Beata Turoňová +7 more
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Structure of the human marker of self 5-transmembrane receptor CD47
Nature Communications, 2021 CD47 is a transmembrane receptor involved in the regulation of various signalling pathways and a promising target for immuno-oncology therapeutics. Here, the authors present the crystal structure of full-length human CD47 and provide insights into the ...
Gustavo Fenalti +15 more
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Adipocyte HIF2α functions as a thermostat via PKA Cα regulation in beige adipocytes
Nature Communications, 2022 Thermogenic adipocytes maintain body temperature in response to cold, but how this is tuned during cold and re-warming is unclear. Here, the authors show HIF2α inhibits beige adipocyte retention, regulating PKA catalysis to control dynamic adipocyte ...
Ji Seul Han +14 more
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Structure of human TRPM8 channel
Communications Biology, 2023 TRPM8 is a non-selective cation channel permeable to both monovalent and divalent cations that is activated by multiple factors, such as temperature, voltage, pressure, and changes in osmolality. It is a therapeutic target for anticancer drug development,
Sergii Palchevskyi +7 more
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Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision
Nature Communications, 2020 Single molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100 ...
Rohit Agarwal, Karl E. Duderstadt
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Molecular Metabolism, 2014 Background/objectives: Fasting dyslipidemia is commonly observed in insulin resistant states and mechanistically linked to hepatic overproduction of very low density lipoprotein (VLDL).
Jennifer Taher +9 more
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Characterisation of HOIP RBR E3 ligase conformational dynamics using integrative modelling
Scientific Reports, 2022 Multidomain proteins composed of individual domains connected by flexible linkers pose a challenge for structural studies due to their intrinsic conformational dynamics.
Marius Kausas +3 more
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