Results 161 to 170 of about 84,981 (250)

Immune Complexes Shape the Pathogenicity of Autoantibodies in Systemic Autoimmune Diseases: Insights From an FcγRIIIA‐Reporter Assay

Arthritis &Rheumatology, EarlyView.
Objective Immune complexes (ICs), formed by autoantigen and autoantibody, play a pathogenic role in systemic autoimmune diseases through stimulation of Fcγ receptors (FcγR). However, studies investigating bioactivity of circulating ICs across various diseases remain limited.
Koji Suzuki, Masaru Takeshita, Yuki Imai, Hiroyuki Fukui, Kazunori Furuhashi, Kotaro Matsumoto, Jun Kikuchi, Keiko Yoshimoto, Yuko Kaneko   +8 more
wiley   +1 more source

Analysis of Single Particles of Amyloid Beta and α‐Synuclein With Seeded Amplification for the Diagnosis of Alzheimer's and Parkinson's Disease

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Neurodegenerative diseases, including Alzheimer's disease (AD) and Parkinson's disease (PD), are characterized by the pathological aggregation of specific proteins such as amyloid beta (Aβ) and α‐synuclein, respectively. Early detection of these protein aggregates in biological fluids could facilitate timely diagnosis and therapeutic ...
Alexandra Dybala, Marlene Pils, Oliver Bannach, Gültekin Tamgüney, Detlev Riesner   +4 more
wiley   +1 more source

Targeted photodynamic therapy of breast cancer cells using antibody–phthalocyanine–gold nanoparticle conjugates [PDF]

, 2011
Cook, MJ, Edwards, DR, Moreno, M, Russell, DA, Stuchinskaya, T   +4 more
core   +1 more source

Transient Transfection of Rolling‐Circle Amplified DNA in Biomanufacturing‐Relevant Mammalian Cell Lines: A Comparison of Transfection Conditions for Optimal Protein Expression

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Rolling‐circle amplification (RCA) is a completely synthetic and rapid mechanism for scaling‐up bulk DNA that eliminates requirements for large‐scale bacterial fermentation. Several reports have described the surprising ability of large unprocessed (hyperbranched) rolling circle‐amplified DNA (RCA DNA) to effectively transfect cultured cells ...
Evelina Loghin, Erik Kvam, Lisa Lowery, Nathan Hudson, Jenny Keen, Brian Davis, Roger B. Dodd, Christine Surrette, John Nelson   +8 more
wiley   +1 more source

Design of experiments‐driven optimization of Porphyridium marinum cultivation: impact of operational variables on phycoerythrin and exopolysaccharide production

Biofuels, Bioproducts and Biorefining, EarlyView.
Abstract Among the Porphyridium genus, Porphyridium marinum exhibits the highest phycoerythrin (PE) content. In this study, the metabolic trade‐off between biomass, PE, and sulfated exopolysaccharide (EPS) production was assessed under varying nitrogen and sulfur availability, light intensity, residence time, and cultivation mode.
Rosaria Tizzani, Agnese Torrisi, Fabrizio Bezzo, Eleonora Sforza   +3 more
wiley   +1 more source

The effect of pretreatment on VFA production from tofu and tempeh wastewater through anaerobic digestion batch

Biofuels, Bioproducts and Biorefining, EarlyView.
Abstract Tofu and tempeh, derived from soybeans, are widely consumed for their nutritional value and high protein content. However, the production of these foods generates nutrient‐rich wastewater that poses environmental challenges while offering opportunities for valorization.
Lydia Mawar Ningsih, Mohammad J. Taherzadeh, Steven Wainaina, Hynek Roubík   +3 more
wiley   +1 more source

Monomeric C-Reactive Protein Aggravates Secondary Degeneration after Intracerebral Haemorrhagic Stroke and May Function as a Sensor for Systemic Inflammation. [PDF]

J Clin Med, 2020
Slevin M, García-Lara E, Capitanescu B, Sanfeliu C, Zeinolabediny Y, AlBaradie R, Olah P, Guo B, Pirici D, Di Napoli M, Popa-Wagner A.   +10 more
europepmc   +1 more source

Development of Raman Spectroscopy and Machine Learning Methods for Protein Aggregate Quantification: Application to BSA in Chromatographic Processes

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Protein aggregation poses a significant risk to biopharmaceutical product quality, as even minor amounts of oligomeric species can compromise efficacy and safety. Rapid and reliable detection of protein aggregates thus remains a major challenge in biopharmaceutical manufacturing.
Jakob Heyer‐Müller, Robin Schiemer, Lars Robbel, Michael Schmitt, Jürgen Hubbuch   +4 more
wiley   +1 more source

Quantitative Analysis Reveals Hitchhiking Drives Polysorbate Hydrolase Persistence Via Host Cell Protein–Antibody Interactions

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Polysorbate‐degrading host cell proteins (HCPs) represent a critical challenge in the manufacturing of monoclonal antibody therapeutics due to their potential to persist during downstream processing. While their enzymatic activity has been characterized, the role of direct HCP‐mAb interactions, particularly those involving polysorbate ...
Melanie Maier, Lukas Griesinger, Matthias Franzreb, Simon Kluters   +3 more
wiley   +1 more source

Engineered Bacterial Ligand From Streptococcus pneumoniae on Macroporous Resins for Selective Affinity Capture of Secretory IgA

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Secretory immunoglobulin A (sIgA) is a promising emerging biopharmaceutical candidate, but it currently lacks a standardized platform for purification. To address this, a novel affinity chromatography resin was developed by immobilizing protein variants of the Streptococcus pneumoniae surface protein, SpsA, as an affinity ligand.
David Scheich, Andres Männik, Oliver Meikar, Joan Teyra, Laura Kibena, Sebastiaan Eeltink, Rainer Hahn, Alois Jungbauer, Nico Lingg   +8 more
wiley   +1 more source