Pathogenic Gαo Mutants Drive Dominant GPCR Coupling in GNAO1 Encephalopathies. [PDF]
FASEB JMutations in GNAO1, the gene encoding Gαo, are associated with a wide range of neurodevelopmental disorders. In this study, we show that Gαo variants linked to the most severe patient phenotypes fail to disengage from activated Gi/o‐coupled GPCRs, thereby blocking GRK‐mediated receptor phosphorylation and internalization.
Larasati YA +4 more
europepmc +2 more sources
Potassium acts as a GTPase-activating element on each nucleotide-binding domain of the essential Bacillus subtilis EngA. [PDF]
PLoS ONE, 2012 EngA proteins form a unique family of bacterial GTPases with two GTP-binding domains in tandem, namely GD1 and GD2, followed by a KH (K-homology) domain.
Anne-Emmanuelle Foucher +4 more
doaj +1 more source
Biochemical characterization of highly purified leucine-rich repeat kinases 1 and 2 demonstrates formation of homodimers. [PDF]
PLoS ONE, 2012 Leucine-rich repeat kinase 1 and 2 (LRRK1 and LRRK2) are large multidomain proteins containing kinase, GTPase and multiple protein-protein interaction domains, but only mutations in LRRK2 are linked to familial Parkinson's disease (PD).
Laura Civiero +13 more
doaj +1 more source
The nucleotide exchange factor Ric-8A is a chaperone for the conformationally dynamic nucleotide-free state of Gαi1. [PDF]
PLoS ONE, 2011 Heterotrimeric G protein α subunits are activated upon exchange of GDP for GTP at the nucleotide binding site of Gα, catalyzed by guanine nucleotide exchange factors (GEFs). In addition to transmembrane G protein-coupled receptors (GPCRs), which act on G
Celestine J Thomas +7 more
doaj +1 more source
Role for Rab10 in Methamphetamine-Induced Behavior. [PDF]
PLoS ONE, 2015 Lipid rafts are specialized, cholesterol-rich membrane compartments that help to organize transmembrane signaling by restricting or promoting interactions with subsets of the cellular proteome.
Scott M Vanderwerf +6 more
doaj +1 more source
Nanoclustering as a dominant feature of plasma membrane organization [PDF]
, 2014 Early studies have revealed that some mammalian plasma membrane proteins exist in small nanoclusters. The advent of super-resolution microscopy has corroborated and extended this picture, and led to the suggestion that many, if not most, membrane ...
Cambi, Alessandra +4 more
core +11 more sources
Extensile Active Hydrogels Driven by Living FtsZ Polymers. [PDF]
SmallUpon post‐gelation activation with Mg2⁺/GTP, living FtsZ polymers treadmill inside a polyacrylamide network, generating extensile internal stresses that drive isotropic swelling and softening. Interpreting mechanics against a Flory–Rehner swelling baseline separates passive dilution from genuine activity, revealing an FR‐normalized permittivity that ...
Kharbedia M +8 more
europepmc +2 more sources
Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif [PDF]
, 2017 Heterotrimeric G proteins are quintessential signalling switches activated by nucleotide exchange on Gα. Although activation is predominantly carried out by G-protein-coupled receptors (GPCRs), non-receptor guanine-nucleotide exchange factors (GEFs) have
Baillie, George S. +14 more
core +1 more source
A gradient-forming MipZ protein mediating the control of cell division in the magnetotactic bacterium Magnetospirillum gryphiswaldense [PDF]
, 2019 Cell division needs to be tightly regulated and closely coordinated with other cellular processes to ensure the generation of fully viable offspring.
Bramkamp, M. +8 more
core +1 more source
Analysis of Binding Site Hot Spots on the Surface of Ras GTPase [PDF]
, 2011 We have recently discovered an allosteric switch in Ras, bringing an additional level of complexity to this GTPase whose mutants are involved in nearly 30% of cancers.
Buhrman, Greg +9 more
core +2 more sources