Results 41 to 50 of about 66,572 (210)

Cloning, expression and purification of the general stress protein Yhbo from Escherichia coli [PDF]

Protein expression and purification. sous presse (2005) ?, 2005
We cloned, expressed and purified the Escherichia coli yhbO gene product, which is homolog to the Bacillus subtilis general stress protein 18 (the yfkM gene product), the Pyrococcus furiosus intracellular protease PfpI, and the human Parkinson disease protein DJ-1. The gene coding for YhbO was generated by amplifying the yhbO gene from E.
arxiv   +1 more source

Structural insight into TPX2-stimulated microtubule assembly [PDF]

, 2017
During mitosis and meiosis, microtubule (MT) assembly is locally upregulated by the chromatin-dependent Ran-GTP pathway. One of its key targets is the MT-associated spindle assembly factor TPX2.
Nogales, Eva, Roostalu, Johanna, Surrey, Thomas, Zhang, Rui   +3 more
core   +2 more sources

Polymeric Assembly of Gluten Proteins in an Aqueous Ethanol Solvent [PDF]

J. Phys. Chem. B 2014, 118, 11065, 2014
The supramolecular organization of wheat gluten proteins is largely unknown due to the intrinsic complexity of this family of proteins and their insolubility in water. We fractionate gluten in a water/ethanol (50/50 v/v) and obtain a protein extract which is depleted in gliadin, the monomeric part of wheat gluten proteins, and enriched in glutenin, the
arxiv   +1 more source

The Cool-2/α-Pix Protein Mediates a Cdc42-Rac Signaling Cascade [PDF]

, 2005
Background:Cloned-out of library-2 (Cool-2)/PAK-interactive exchange factor (α-Pix) was identified through its ability to bind the Cdc42/Rac target p21-activated kinase (PAK) and has been implicated in certain forms of X-linked mental retardation as well
Baird, Dan, Feng, Qiyu, Cerione, Richard A.   +2 more
core   +3 more sources

Mitochondrial dysfunction: Related diseases, influencing factors, and detection

Interdisciplinary Medicine, EarlyView.
Mitochondrial dysfunction is implicated in the pathogenesis of numerous diseases, including neurological disorders, cancers, and cardiovascular conditions, through mechanisms such as mitochondrial DNA mutations, dysregulation of mitochondrial network dynamics, and impaired mitophagy.
Zhaojin Li, Shiyu Li, Wanying Chen, Ziyu Zhang, Chun Pan, Peng Lei, Cheng Cheng, Junyou Zhu, Hanxiao Sun, Zhenning Dai   +9 more
wiley   +1 more source

Cellular localization, accumulation and trafficking of double-walled carbon nanotubes in human prostate cancer cells [PDF]

, 2012
Carbon nanotubes (CNTs) are at present being considered as potential nanovectors with the ability to deliver therapeutic cargoes into living cells. Previous studies established the ability of CNTs to enter cells and their therapeutic utility, but an ...
A. T. Jones, Andreas Gerondopoulos, B. M. Mullock, B. Soula, C. Bucci, C. N. Connolly, Carmen Tîlmaciu, D. J. Yamashiro, D. Pantarotto, E. F. C. Blommaart, E. Heister, E. Heister, Elena Heister, Emmanuel Flahaut, F. Aniento, F. R. Maxfield, F. Zhou, G. Bartholomeusz, G. Li, H. Jin, H. Jin, H. N. Yehia, H. W. Davidson, Helen M. Coley, J. F. Clarke, J. P. Luzio, J. Saraste, Johnjoe McFadden, K. Kostarelos, L. Lacerda, L. Lacerda, M. Cardone, M. J. Clague, M. J. Quon, M. L. Wei, M. Ren, M. S. Strano, N. W. S. Kam, P. O. Seglen, P. Saftig, Q. Mu, R. G. Parton, R. N. Ghosh, R. Saito, S. K. Doorn, S. Ravi P. Silva, V. Neves, Vera Neves, W. J. Brown, Y. Wu   +49 more
core   +3 more sources

The Actin‐Binding Prolyl‐Isomerase Par17 Sustains Its Substrate Selectivity by Interdomain Allostery

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT The human peptidyl‐prolyl‐cis/trans isomerases (PPIases), Parvulin 14 and Parvulin 17, accelerate the cis/trans isomerization of Xaa‐Pro moieties within protein sequences. By modulating the respective binding interfaces of their target proteins, they play a crucial role in determining the fate of their substrates within the cell. Although both
Anna Sternberg, Jennifer Lynne Borger, Mathilda Thies, Anja Matena, Mike Blueggel, Bianca E. Kamba, Christine Beuck, Farnusch Kaschani, Markus Kaiser, Peter Bayer   +9 more
wiley   +1 more source

Delicate Analysis of Interacting Proteins and Their Assemblies by Flow Field-Flow Fractionation Techniques [PDF]

Biomacromolecules, 2024, 25, pp.3976 - 3989
We study the efficiency of several Asymmetrical Flow Field-Flow Fractionation (AF4) techniques to investigate the self-associating wheat gluten proteins. We compare the use of a denaturing buffer including sodium dodecyl sulfate (SDS) and a mild chaotropic solvent, water/ethanol, as eluent, on a model gluten sample.
arxiv   +1 more source

Structural entanglements in protein complexes [PDF]

The Journal of Chemical Physics, 146(22), 225102, 2017
We consider multi-chain protein native structures and propose a criterion that determines whether two chains in the system are entangled or not. The criterion is based on the behavior observed by pulling at both temini of each chain simultaneously in the two chains.
arxiv   +1 more source

Luteinizing hormone receptor knockout mouse: What has it taught us?

Andrology, EarlyView.
Abstract Luteinizing hormone (LH), along with its agonist choriongonadotropin (hCG) in humans, is the key hormone responsible for the tropic regulation of the gonadal function. LH and hCG act through their cognate receptor, the luteinizing hormone/choriongonadotropin receptor (LHCGR; more appropriately LHR in rodents lacking CG), located in the testis ...
Ilpo T. Huhtaniemi
wiley   +1 more source