The COOH terminus of the c-Abl tyrosine kinase contains distinct F- and G-actin binding domains with bundling activity [PDF]
, 1994 The myristoylated form of c-Abl protein, as well as the P210bcr/abl protein, have been shown by indirect immunofluorescence to associate with F-actin stress fibers in fibroblasts. Analysis of deletion mutants of c-Abl stably expressed in fibroblasts maps
Baltimore, David +5 more
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Neck linker docking is critical for Kinesin-1 force generation in cells but at a cost to motor speed and processivity. [PDF]
, 2019 Kinesin force generation involves ATP-induced docking of the neck linker (NL) along the motor core. However, the roles of the proposed steps of NL docking, cover-neck bundle (CNB) and asparagine latch (N-latch) formation, during force generation are ...
Budaitis, Breane G +8 more
core +1 more source
The protein import machinery of chloroplasts [PDF]
, 1995 Mathis, Paul, Seedorf, M., Soll, Jürgen
core +1 more source
Protein fluorescence of nicotinamide nucleotide-dependent dehydrogenases [PDF]
, 1972 Evans, RW +5 more
core
Nascent polypeptide chains on mitochondrial ribosomes and their integration into the inner membrane [PDF]
, 1974 Kroon, Albertus M. +3 more
core
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Molecular analysis of the monomeric GTP-binding proteins of oligodendrocytes
Molecular Brain Research, 1997Vesicle transport plays an important role in the formation of myelin. Transport of proteins, including proteolipid protein and myelin associated glycoprotein, from their site of synthesis in the endoplasmic reticulum in the perikaryon of the oligodendrocytes, to myelin, takes place via carrier vesicles. The mechanisms that regulate vesicle transport in
R Burcelin +2 more
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The control of plant growth, differentiation, and development is considered in relation to the involvement of monomeric GTP-binding proteins (mG-proteins) in the extra-and intracellular signal transduction. The principal attention is paid to Rop mG-proteins, unique small GTPases of eukaryotic cells functioning during various developmental stages of ...
I E Moshkov
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An Expression Cloning Method to Identify Monomeric GTP-Binding Proteins by GTP Overlay
Analytical Biochemistry, 1997We have developed a method for identifying monomeric GTP-binding proteins that is based on probing plasmid expression libraries with [alpha-32P]GTP. The method involves the production of nitrocellulose replica filter lifts from a plasmid cDNA expression library and treatment of the filters with chloroform vapor to lyse the Escherichia coli and to ...
Douglas A Andres
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A cDNA Cloning Method for Monomeric GTP-Binding Proteins by Ligand Blotting
Analytical Biochemistry, 1993We have developed a convenient method of cDNA cloning for monomeric GTP-binding proteins. The method consists of a serial dilution of cDNA expression library, a separation of proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and a ligand blotting; each group containing the proteins from about 1000 independent colonies ...
Yukio Nagano, R Matsuno
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The monomeric GTP binding protein, rab3a, is associated with the acrosome in mouse sperm
Molecular Reproduction and Development, 1999Exocytosis of the sperm acrosome is an obligate precursor to successful egg penetration and subsequent fertilization. In most mammals, acrosomal exocytosis occurs at a precise time, after sperm binding to the zona pellucida of the egg, and is induced by a specific component of the zona pellucida.
C R, Ward, D, Faundes, J A, Foster
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