Results 31 to 40 of about 637 (211)

Comparative sequence analysis as a tool for studying the secondary structure of mRNAs [PDF]

Nucleic Acids Research, 1985
Analysis of phylogenetically conserved secondary structure has been important in the development of models for the secondary structure of structural RNAs. In this paper, we apply this type of analysis to several families of informational RNAs to evaluate its usefulness in developing secondary structure models for mRNAs and mRNA precursors.
M F, Browner, C B, Lawrence
openaire   +2 more sources

Making ends meet: new functions of mRNA secondary structure [PDF]

WIREs RNA, 2020
Abstract The 5’ cap and 3’ poly(A) tail of mRNA are known to synergistically regulate mRNA translation and stability. Recent computational and experimental studies revealed that both protein-coding and non-coding RNAs will fold with extensive intramolecular secondary structure, which will result in close distances between the sequence ...
Dmitri N. Ermolenko, David H. Mathews
openaire   +3 more sources

Reverse Transcription Slippage over the mRNA Secondary Structure of the LIP1 Gene

BioTechniques, 2001
The secondary structures in mRNA often cause early termination during the synthesis of cDNA. In an attempt to determine the 5′-untranslated region (UTR) of the gene LIP1 using the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE), we found ...
Yan-Jin Zhang, Hong-Yi Pan, Shou-Jiang Gao   +2 more
doaj   +1 more source

PRKCE non-coding variants influence on transcription as well as translation of its gene

RNA Biology, 2022
Untranslated regions of the gene play a crucial role in gene expression regulation at mRNA and protein levels. Mutations at UTRs impact expression by altering transcription factor binding, transcriptional/translational efficacy, miRNA-mediated gene ...
Khushbukhat Khan, Sameen Zafar, Amna Hafeez, Yasmin Badshah, Kanza Shahid, Naeem Mahmood Ashraf, Maria Shabbir   +6 more
doaj   +1 more source

Weak 5′-mRNA Secondary Structures in Short Eukaryotic Genes [PDF]

Genome Biology and Evolution, 2012
Experimental studies of translation have found that short genes tend to exhibit greater densities of ribosomes than long genes in eukaryotic species. It remains an open question whether the elevated ribosome density on short genes is due to faster initiation or slower elongation dynamics.
Ding, Yang, Shah, Premal, Plotkin, Joshua B.   +2 more
openaire   +2 more sources

Avian influenza mRNA vaccine encoding hemagglutinin provides complete protection against divergent H5N1 viruses in specific-pathogen-free chickens

Journal of Nanobiotechnology
Background The rapid mutation of avian influenza virus (AIV) poses a significant threat to both the poultry industry and public health. Herein, we have successfully developed an mRNA-LNPs candidate vaccine for H5 subtype highly pathogenic avian influenza
Zhaoyang Wang, Chongyu Tian, Jiahang Zhu, Shiqian Wang, Xiang Ao, Yanjuan He, Huixin Chen, Xiuying Liao, Deming Kong, Yongfei Zhou, Wanbo Tai, Ming Liao, Huiying Fan   +12 more
doaj   +1 more source

An mRNA-binding channel in the ES6S region of the translation 48S-PIC promotes RNA unwinding and scanning

eLife, 2019
Loading of mRNA onto the ribosomal 43S pre-initiation complex (PIC) and its subsequent scanning require the removal of the secondary structure of the by RNA helicases such as eIF4A.
Irene Díaz-López, René Toribio, Juan José Berlanga, Iván Ventoso   +3 more
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Dynamics of translation by single ribosomes through mRNA secondary structures [PDF]

Nature Structural & Molecular Biology, 2013
During protein synthesis, the ribosome translates nucleotide triplets in single-stranded mRNA into polypeptide sequences. Strong downstream mRNA secondary structures, which must be unfolded for translation, can slow or even halt protein synthesis.
Chen, Chunlai, Zhang, Haibo, Broitman, Steven L., Reiche, Michael, Farrell, Ian, Cooperman, Barry S., Goldman, Yale E.   +6 more
openaire   +2 more sources

Correlation between the secondary structure of pre-mRNA introns and the efficiency of splicing in Saccharomyces cerevisiae

BMC Genomics, 2008
Background Secondary structure interactions within introns have been shown to be essential for efficient splicing of several yeast genes. The nature of these base-pairing interactions and their effect on splicing efficiency were most extensively studied ...
Rogic Sanja, Montpetit Ben, Hoos Holger H, Mackworth Alan K, Ouellette BF Francis, Hieter Philip   +5 more
doaj   +1 more source