Results 71 to 80 of about 602,475 (301)

Regulation of α-Globin mRNA Stability

Experimental Biology and Medicine, 2003
mRNA stability is a critical determinant of normal red blood cell development and function. The long half-life of globin mRNA is central to the continued synthesis of globin proteins throughout all stages of erythropoiesis, even as the cells undergo programmed transcriptional arrest during terminal differentiation.
Shelly A, Waggoner, Stephen A, Liebhaber
openaire   +2 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

RNA Interference by Single- and Double-stranded siRNA With a DNA Extension Containing a 3′ Nuclease-resistant Mini-hairpin Structure [PDF]

, 2009
Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs.
Allison, Simon J., Milner, Jo
core   +4 more sources

Preconditioning of mesenchymal stromal cells with low-intensity ultrasound: influence on chondrogenesis and directed SOX9 signaling pathways [PDF]

, 2020
Background: Continuous low-intensity ultrasound (cLIUS) facilitates the chondrogenic differentiation of human mesenchymal stromal cells (MSCs) in the absence of exogenously added transforming growth factor-beta (TGFβ) by upregulating the expression of ...
A Argentaro, A Augello, A Katsumi, A Woods, A Woods, AD Miller, AH Huang, B Yang, BH Choi, C Huang, C Matta, CA Simmons, CB Little, CJ O’Conor, CY Huang, D Schumann, DR Haudenschild, EM Darling, F Dell'Accio, G Coricor, H Sim, H-C Hsu, HJ Kwon, HJ Lee, HJ Lee, J Li, J Puetzer, JC Sng, JD Kisiday, JH Cui, JH Cui, JW Choi, K Ebisawa, K Pelttari, L Bian, Li Ling, LJ Ng, M Shakibaei, M Tsuda, MA Corrigan, MB Mueller, N Fahy, N Thelin, NP Whitney, P Müller, R Ogawa, RL Mauck, RM Delaine-Smith, RS Tigli, S Murakami, S Noriega, S Noriega, S Yamaguchi, SD Cook, SD Cook, T Furumatsu, T Guo, T Ito, T Juhasz, T Nishida, T Sanjukta Guha, TM Louw, V Lefebvre, V Lefebvre, W Huang, W Huang, Z Li, ZJ Zhang   +67 more
core   +2 more sources

An mRNA Stability Complex Functions with Poly(A)-Binding Protein To Stabilize mRNA In Vitro [PDF]

Molecular and Cellular Biology, 1999
The stable globin mRNAs provide an ideal system for studying the mechanism governing mammalian mRNA turnover. alpha-Globin mRNA stability is dictated by sequences in the 3' untranslated region (3'UTR) which form a specific ribonucleoprotein complex (alpha-complex) whose presence correlates with mRNA stability. One of the major protein components within
Z, Wang, N, Day, P, Trifillis, M, Kiledjian   +3 more
openaire   +2 more sources

Integrated genomic and proteomic profiling reveals insights into chemoradiation resistance in cervical cancer

Molecular Oncology, EarlyView.
A comprehensive genomic and proteomic analysis of cervical cancer revealed STK11 and STX3 as a potential biomarkers of chemoradiation resistance. Our study demonstrated EGFR as a therapeutic target, paving the way for precision strategies to overcome treatment failure and the DNA repair pathway as a critical mechanism of resistance.
Janani Sambath, Irene A. George, Srikanth S. Manda, Prasanth Ariyannur, Ekta R. Dhawale, Raja Sekhar Kommu, Rajan Datar, Darshana Patil, Vinita Trivedi, Manisha Singh, Kumar Prabhash, Sewanti Limaye, Richa Chauhan, Prashant Kumar   +13 more
wiley   +1 more source

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

CUG-BP and 3'UTR sequences influence PARN-mediated deadenylation in mammalian cell extracts

Genetics and Molecular Biology, 2007
Several mRNAs have been shown to exhibit distinct patterns of poly(A) shortening prior to their decay in vivo. In this study, we show that individual transcripts also demonstrate distinct patterns of deadenylation in in vitro systems derived from HeLa ...
Karen C.M. Moraes, Carol J. Wilusz, Jeffrey Wilusz   +2 more
doaj   +1 more source

Time-delayed models of gene regulatory networks [PDF]

, 2015
We discuss different mathematical models of gene regulatory networks as relevant to the onset and development of cancer. After discussion of alternativemodelling approaches, we use a paradigmatic two-gene network to focus on the role played by time ...
Blyuss, K B, Hogan, S J, Kyrychko, Y N, Parmar, K   +3 more
core   +4 more sources

mRNA stability in mammalian cells

Microbiological Reviews, 1995
This review concerns how cytoplasmic mRNA half-lives are regulated and how mRNA decay rates influence gene expression. mRNA stability influences gene expression in virtually all organisms, from bacteria to mammals, and the abundance of a particular mRNA can fluctuate manyfold following a change in the mRNA half-life, without any change in transcription.
openaire   +2 more sources