The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols [PDF]
Open Veterinary Journal, 2021 The MTT reduction assay is one of the methods used to evaluate the viability of sperm. In the assay, a tetrazolium component (MTT) is converted into MTT formazan by some specific enzymes in the viable cells.
Kakanang Buranaamnuay
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Improved Formazan Dissolution for Bacterial MTT Assay [PDF]
Microbiology Spectrum, 2021 The MTT assay, based on the enzymatic reduction of the water-soluble, yellowish tetrazolium salt 3-(4,5-dimethylthiazol)-2,5-diphenyl-tetrazolium bromide (MTT) to purple formazan, is commonly used for assessment of cell viability and proliferation ...
Ludmil Benov
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Interference of Phenylethanoid Glycosides from Cistanche tubulosa with the MTT Assay [PDF]
Molecules, 2015 The MTT assay, as a screening method, has been widely used to measure the viability and proliferation of cells. However, it should be noted that MTT assay may not accurately reflect the effect of Cistanche tubulosa ethanolic extract on EA.hy926 cells ...
Yu-Jie Wang +3 more
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The MTT Assay: Utility, Limitations, Pitfalls, and Interpretation in Bulk and Single-Cell Analysis [PDF]
International Journal of Molecular Sciences, 2021 The MTT assay for cellular metabolic activity is almost ubiquitous to studies of cell toxicity; however, it is commonly applied and interpreted erroneously.
Ivan Kempson +2 more
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Mathematical Modeling for an MTT Assay in Fluorine-Containing Graphene Quantum Dots [PDF]
Nanomaterials, 2022 The paper reports on a new mathematical model, starting with the original Hill equation which is derived to describe cell viability (V) while testing nanomaterials (NMs).
Paulo C. Morais, Dieime C. Silva
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Quantitative evaluation of human sperm viability using MTT assay: A laboratory study [PDF]
International Journal of Reproductive BioMedicine, 2020 Background: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability.
Hamid Reza Momeni +2 more
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Applied Sciences, 2021 Photodynamic therapy (PDT) combines light, a photosensitizing chemical substance, and molecular oxygen to elicit cell death and is employed in the treatment of a variety of diseases, including cancer.
Edith Alejandra Carreño +5 more
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Pitfalls and other issues with the MTT assay
Photodiagnosis and Photodynamic TherapyWhile the colorimetric assay commonly referred to as ‘MTT’ does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay.
David Kessel
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Evaluation of Direct Colorimetric MTT Assay for Rapid Detection of Rifampicin and Isoniazid Resistance in Mycobacterium tuberculosis. [PDF]
PLoS ONE, 2016 With the spread of multidrug-resistant tuberculosis (MDR-TB) strains there is an increasing need for new accurate and cost-effective methods for a rapid diagnostic and drug susceptibility testing (DST), particularly in low-income countries where ...
Gadissa Bedada Hundie +2 more
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Cytotoxicity of some 1-(2,4-dihydroxyphenyl)-3-(4-phenylpiperidin-1-yl) prop-2-en-1-one derivatives using MTT assay [PDF]
Trends in Pharmaceutical Sciences, 2015 Some N-substituted piperidine structures were synthesized and evaluated for cytotoxic activity against four different cell lines using the standard MTT assay method and Doxorubicin was used as the reference drug.
Farzaneh Fesahat +5 more
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