Results 11 to 20 of about 1,060,750 (286)

The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocols [PDF]

open access: yesOpen Veterinary Journal, 2021
The MTT reduction assay is one of the methods used to evaluate the viability of sperm. In the assay, a tetrazolium component (MTT) is converted into MTT formazan by some specific enzymes in the viable cells.
Kakanang Buranaamnuay
doaj   +7 more sources

Improved Formazan Dissolution for Bacterial MTT Assay [PDF]

open access: yesMicrobiology Spectrum, 2021
The MTT assay, based on the enzymatic reduction of the water-soluble, yellowish tetrazolium salt 3-(4,5-dimethylthiazol)-2,5-diphenyl-tetrazolium bromide (MTT) to purple formazan, is commonly used for assessment of cell viability and proliferation ...
Ludmil Benov
doaj   +5 more sources

Interference of Phenylethanoid Glycosides from Cistanche tubulosa with the MTT Assay [PDF]

open access: yesMolecules, 2015
The MTT assay, as a screening method, has been widely used to measure the viability and proliferation of cells. However, it should be noted that MTT assay may not accurately reflect the effect of Cistanche tubulosa ethanolic extract on EA.hy926 cells ...
Yu-Jie Wang   +3 more
doaj   +4 more sources

Mathematical Modeling for an MTT Assay in Fluorine-Containing Graphene Quantum Dots [PDF]

open access: yesNanomaterials, 2022
The paper reports on a new mathematical model, starting with the original Hill equation which is derived to describe cell viability (V) while testing nanomaterials (NMs).
Paulo C. Morais, Dieime C. Silva
doaj   +2 more sources

Detection of Microorganisms in Body Fluids via MTT-PMS Assay

open access: yesDiagnostics, 2021
Early detection of microorganisms is essential for the management of infectious diseases. However, this is challenging, as traditional culture methods are labor-intensive and time-consuming.
Cheng-Han Chen   +8 more
doaj   +2 more sources

Quantitative evaluation of human sperm viability using MTT assay: A laboratory study [PDF]

open access: yesInternational Journal of Reproductive BioMedicine, 2020
Background: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability.
Hamid Reza Momeni   +2 more
doaj   +2 more sources

Considerations and Technical Pitfalls in the Employment of the MTT Assay to Evaluate Photosensitizers for Photodynamic Therapy

open access: yesApplied Sciences, 2021
Photodynamic therapy (PDT) combines light, a photosensitizing chemical substance, and molecular oxygen to elicit cell death and is employed in the treatment of a variety of diseases, including cancer.
Edith Alejandra Carreño   +5 more
doaj   +3 more sources

Pitfalls and other issues with the MTT assay

open access: yesPhotodiagnosis and Photodynamic Therapy
While the colorimetric assay commonly referred to as ‘MTT’ does provide an indication of effects of photodamage on mitochondrial enzyme levels, a careful analysis showed that the resulting data were not correlated with a clonogenic assay.
David Kessel
doaj   +3 more sources

Evaluation of Direct Colorimetric MTT Assay for Rapid Detection of Rifampicin and Isoniazid Resistance in Mycobacterium tuberculosis. [PDF]

open access: yesPLoS ONE, 2016
With the spread of multidrug-resistant tuberculosis (MDR-TB) strains there is an increasing need for new accurate and cost-effective methods for a rapid diagnostic and drug susceptibility testing (DST), particularly in low-income countries where ...
Gadissa Bedada Hundie   +2 more
doaj   +2 more sources

MTT assay to evaluate the cytotoxic potential of a drug

open access: yesBangladesh Journal of Pharmacology, 2017
Quantification of cell viability and proliferation form the fundamental for numerous in vitro assays in response to external factors. An MTT assay is a colorimetric assay based on assessing the cell metabolic activity.
Ashutosh Bahuguna   +3 more
doaj   +2 more sources

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