Results 151 to 160 of about 3,358,336 (306)

Epitope-specificity repertoire of CD4⁺ and CD8⁺ T cells in the lungs.

, 2019
For experimental design and codes of experimental groups, see Fig 1A and Table 1. Frequencies of epitope-specific cells among immunomagnetically-purified CD4+ and CD8+ T cells, retrieved from lung tissue at 48 hrs after the last challenge exposure to OVA
Jürgen Podlech (339202), Hendrik Beckert (6293033), Matthias J. Reddehase (139680), Daniel Teschner (6293024), Roland Buhl (347962), Frederic Ries (6293030), Christian Taube (35629), Sebastian Reuter (347960), Joachim Maxeiner (6432917), Rafaela Holtappels (555480), Niels A. W. Lemmermann (437752), Kirsten Freitag (270256)   +11 more
core   +1 more source

Multi-epitope vaccine design for hepatitis E virus based on protein ORF2 and ORF3

Frontiers in Microbiology
IntroductionHepatitis E virus (HEV), with heightened virulence in immunocompromised individuals and pregnant women, is a pervasive threat in developing countries.
Qiong Lu, Hao Wu, Hao Wu, Jing Meng, Jiangyuan Wang, Jiajing Wu, Shuo Liu, Shuo Liu, Jincheng Tong, Jianhui Nie, Weijin Huang   +10 more
doaj   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Expression and immunogenicity evaluation of a novel Lentiviral multi- epitope vaccine against Leishmania major in BALB/c mice

Tropical Diseases, Travel Medicine and Vaccines
Background Nowadays, the prevention of parasitic diseases including leishmaniasis, particularly cutaneous leishmaniasis as the smost common type of the disease, has increased health concerns around the world.
Mahsa Rabienia, Zahra Roudbari, Ali Ghanbariasad, Abdolmajid Ghasemian, Akbar Farjadfar, Nahid Mortazavidehkordi   +5 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

Multi-epitope Melanoma Peptide Vaccine [PDF]

, 2020
openaire   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source