Results 11 to 20 of about 137,324 (248)

Clinical use of multiplex-PCR for the diagnosis of acute bacterial meningitis

Journal of Family Medicine and Primary Care, 2022
Background and Objectives: Prompt and accurate diagnosis of acute bacterial meningitis (ABM) is critical for patient management. We designed and evaluated two sets of multiplex-PCR assays for the simultaneous detection of six major etiologies of ABM i.e.,
Nupur Sharma, Hitender Gautam, Sonu Tyagi, Shahid Raza, Sarita Mohapatra, Seema Sood, Benu Dhawan, Arti Kapil, Bimal K Das   +8 more
doaj   +1 more source

Development of multiplex PCR for rapid detection of metallo-β-lactamase genes in clinical isolates of Acinetobacter baumannii

Iranian Journal of Microbiology, 2020
Background and Objectives: Acinetobacter baumannii has been known as a major pathogen causing nosocomial infections. The aim of this study was to develop multiplex PCR for rapid and simultaneous detection of metallo-β-lactamase (MBL) genes in clinical ...
Reza Ranjbar, Shahin Zayeri, Amir Mirzaie   +2 more
doaj   +1 more source

Molecular diagnosis of the human immunodeficiency, Hepatitis B and C viruses among blood donors in Lomé (Togo) by multiplex real time PCR

The Pan African Medical Journal, 2016
This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo.
Maleki Assih, Lochina Feteke, Cyrille Bisseye, Djeneba Ouermi, Florencia Djigma, Simplice Damintoti Karou, Jacques Simpore   +6 more
doaj   +1 more source

Multiplex PCR: Critical Parameters and Step-by-Step Protocol

BioTechniques, 1997
By simultaneously amplifying more than one locus in the same reaction, multiplex PCR is becoming a rapid and convenient screening assay in both the clinical and the research laboratory.
O. Henegariu, N.A. Heerema, S.R. Dlouhy, G.H. Vance, P.H. Vogt   +4 more
doaj   +1 more source

Fragment Size Difference between Multiplex and Singleplex PCR Products and Their Practical Implications

BioTechniques, 2000
By simultaneously amplifying several loci in the same reaction, multiplex PCR has been used in gene mapping and DNA typing with polymorphic short tandem repeat loci.
H.-W. Deng, Y. Zhou, R.R. Recker, M.L. Johnson, J. Li   +4 more
doaj   +1 more source

primerJinn: a tool for rationally designing multiplex PCR primer sets for amplicon sequencing and performing in silico PCR

BMC Bioinformatics, 2023
Background Multiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of its most important applications is in the targeted sequencing of pathogens.
Jason D. Limberis, John Z. Metcalfe
doaj   +1 more source

Development of a Multiplex Crystal Digital RT-PCR for Differential Detection of Classical, Highly Pathogenic, and NADC30-like Porcine Reproductive and Respiratory Syndrome Virus

Animals, 2023
Porcine reproductive and respiratory syndrome virus (PRRSV) type 1 (European genotype) and PRRSV type 2 (North American genotype) are prevalent all over the world.
Feng Long, Yating Chen, Kaichuang Shi, Yanwen Yin, Shuping Feng, Hongbin Si   +5 more
doaj   +1 more source

Multiplex PCR theranostics of severe respiratory infections [PDF]

Expert Review of Anti-infective Therapy, 2010
Acute respiratory tract infection is the lead-ing cause of hospitalization for infants and young children in developed countries, and a major determinant of death in develop-ing countries. In 2002, lower respiratory tract infections accounted for approxi-mately 4 million deaths worldwide and 7% of all deaths that ...
Pozzetto, Bruno, Grattard, Florence, Pillet, Sylvie   +2 more
openaire   +4 more sources

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.)

Silva Fennica, 2016
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube.
Nevenka Ćelepirović, Monika Karija Vlahović, Aikaterini Dounavi, Mladen Ivanković   +3 more
doaj   +1 more source

Cryptococcusspecies identification by multiplex PCR [PDF]

Medical Mycology, 2008
Members of the Cryptococcus species complex are encapsulated basidiomycetous yeasts, which can affect the central nervous system (CNS) and if untreated may cause meningitis. Cryptococcus neoformans is an opportunistic pathogen causing infections mainly in immunocompromised individuals.
Ana Lusia, Leal, Josiane, Faganello, Maria Cristina, Bassanesi, Marilene H, Vainstein   +3 more
openaire   +2 more sources