Results 221 to 230 of about 137,324 (248)

Real-Time Multiplex PCR Assays

Methods, 2001
The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra.
C T, Wittwer, M G, Herrmann, C N, Gundry, K S, Elenitoba-Johnson   +3 more
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Multiplex PCR Design for Scalable Resequencing

2021
While conventional PCR applications typically focus on a single PCR assay per reaction, multiplex PCR applications are a convenient and scalable solution becoming more routine. Multiplex methods can be applied to virtually any DNA template source (e.g., plant or human DNA, FFPE DNA isolated from clinical samples, bisulfite-converted DNA for DNA ...
Darren, Korbie, Matt, Trau
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Hydrogels for Efficient Multiplex PCR

Biotechnology and Bioprocess Engineering, 2020
Multiplex PCR simultaneously detects several different DNA targets in a given sample and has, therefore, gained considerable interest for use in examining a genetic target whose number rapidly increases in a single phenotype. Conventional real-time PCR lacks sufficient multiplex capacity, as the limited number of color channels restricts the number of ...
Juny Shin, Cheulhee Jung
openaire   +1 more source

Optimization of Multiplex PCRs

1994
The development of the polymerase chain reaction (PCR) has enabled rapid and efficient analysis of specific DNA sequences (Mullis and Faloona, 1987). Most PCR strategies are designed to amplify one or more target sequences with a single set of oligonucleotide primers.
Jeffrey S. Chamberlain, Joel R. Chamberlain   +1 more
openaire   +1 more source

Multiplex PCR Amplification of Ancient DNA

2011
Multiplex PCR allows the simultaneous amplification of up to dozens of target fragments in a single PCR. It is therefore a powerful tool to obtain many kilobases of continuous sequence from minute amounts of ancient DNA (aDNA), which usually must be amplified in multiple short and overlapping fragments.
Mathias, Stiller, Tara L, Fulton
openaire   +2 more sources

Multiplex PCR for genotypingFlavobacterium columnare

Journal of Fish Diseases, 2019
AbstractRecent research has identified four distinct genetic groups among isolates ofFlavobacterium columnarethrough multilocus phylogenetic analyses; however, there are no quick methods to determine the genotype of an isolate. The objective of this research was to develop a multiplex PCR to rapidly genotypeF. columnareto genetic group.
Benjamin R. LaFrentz, Julio C. García, John P. Shelley   +2 more
openaire   +2 more sources

Multiplex PCR for detection of acquired carbapenemase genes

Diagnostic Microbiology and Infectious Disease, 2011
A rapid and reliable PCR-based technique was developed for detection of genes encoding carbapenemases belonging to different classes. Primers were designed to amplify the following 11 genes: bla(IMP), bla(VIM), bla(NDM), bla(SPM), bla(AIM), bla(DIM), bla(GIM), bla(SIM)bla(KPC), bla(BIC), and bla(OXA-48). Three different multiplex reaction mixtures were
Poirel, Laurent, Walsh, Timothy R., Cuvillier, Vincent, Nordmann, Patrice   +3 more
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Selecting Degenerate Multiplex PCR Primers

2003
Single Nucleotide Polymorphism (SNP) Genotyping is an important molecular genetics technique in the early stages of producing results that will be useful in the medical field. One of the proposed methods for performing SNP Genotyping requires amplifying regions of DNA surrounding a large number of SNP loci. In order to automate a portion of this method
Richard Souvenir, Jeremy Buhler, Gary Stormo, Weixiong Zhang   +3 more
openaire   +1 more source

METHYLENETETRAHYDROFOLATE REDUCTASE (MTHFR) MULTIPLEX – PCR METHOD

Clinical and Investigative Medicine, 2009
Introduction Gene polymorphisms in human methylenetetrahydrofolate reductase (MTHFR) have been linked to the disorders of folate metabolism, and may contribute to such disease as neural tube defect, coronary heart disease, venous thrombosis, and several types of cancers.
H Kary, B M Gilfix
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[Multiplex PCR in clinical microbiology].

Enfermedades infecciosas y microbiologia clinica, 2004
The introduction of molecular biology methods in clinical microbiology laboratories brings important insights to obtain sensitive and specific diagnoses as fast as possible. These methods are not intended for replacement but for complement of the already applied microbiologic methods.
Sebastián, Méndez-Alvarez, Eduardo, Pérez-Roth   +1 more
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