Results 31 to 40 of about 137,324 (248)

Serotyping Streptococcus pneumoniae by Multiplex PCR [PDF]

Journal of Clinical Microbiology, 2003
ABSTRACT The capsule is a major virulence factor of pneumococci, and it was shown that some capsular variants are associated with antimicrobial resistance and certain types of disease. Moreover, pneumococcal capsular typing has received renewed interest since the availability of conjugate vaccines, which include serotypes frequently ...
D A, Brito, M, Ramirez, H, de Lencastre
openaire   +2 more sources

Multiplex PCR: Optimization and Application in Diagnostic Virology [PDF]

Clinical Microbiology Reviews, 2000
SUMMARY PCR has revolutionized the field of infectious disease diagnosis. To overcome the inherent disadvantage of cost and to improve the diagnostic capacity of the test, multiplex PCR, a variant of the test in which more than one target sequence is amplified using more than one pair of primers, has been developed.
Elnifro, E. M., Ashshi, A. M., Cooper, R. J., Klapper, P. E.   +3 more
openaire   +3 more sources

Establishment of multiplex PCR for detection of genes related to Quinolone resistance

Vietnam Journal of Science, Technology and Engineering, 2018
Background/aims: It has been shown that quinolone resistance arises due to mutations in the quinolone resistance-determining regions of the drug targets.
Ngo Tat Trung, Dao Thanh Quyen, Phan Quoc Hoan, Thirumalaisamy P.P. Velavan, Le Huu Song   +4 more
doaj   +1 more source

Multiplex PCRs for Identification of Necrotoxigenic Escherichia coli [PDF]

Journal of Clinical Microbiology, 2003
ABSTRACT Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli
Van Bost, S., Jacquemin, E., Oswald, E., Mainil, Jacques   +3 more
openaire   +5 more sources

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

A new multiplex polymerase chain reaction assay for the identification a panel of bacteria involved in bacteremia

Advanced Biomedical Research, 2013
Background: Throughout the world, bloodstream infections (BSI s ) are associated with high rates of morbidity and mortality. Rapid pathogens identification is central significance for the outcome of the patient than culture techniques for microbial ...
Hossein Fazzeli, Mohammad R Arabestani, Bahram N Esfahani, Farzin Khorvash, Mohammad R Pourshafie, Sharareh Moghim, Hajieh G Safaei, Jamshid Faghri, Amir Azimian   +8 more
doaj   +1 more source

Diagnostic Accuracy of Multiplex Polymerase Chain Reaction in Early Onset Neonatal Sepsis

Children, 2023
Early onset neonatal sepsis is a significant contributor to neonatal morbidity and mortality. Although blood cultures remain the diagnostic gold standard, they detect pathogens in only a minority of suspected cases.
Anja Stein, Daniel Soukup, Peter-Michael Rath, Ursula Felderhoff-Müser   +3 more
doaj   +1 more source

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

FEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf, Nicholas F. Downing, Kaitlyn M. Mills, Samuel C. Metcalfe, Alexander E. Kritzer, Lindsey D. Mayo, Peter C. Hollenhorst   +6 more
wiley   +1 more source

Rapid detection and molecular survey of blaVIM, blaIMP and blaNDM genes among clinical isolates of Acinetobacter baumannii using new multiplex real-time PCR and melting curve analysis

BMC Microbiology, 2019
Background Acinetobacter baumannii is a cosmopolitan bacterium that is frequently reported from hospitalized patients, especially those patients who admitted in the intensive care unit.
Hossein Goudarzi, Elnaz Sadat Mirsamadi, Zohreh Ghalavand, Mojdeh Hakemi Vala, Hamed Mirjalali, Ali Hashemi   +5 more
doaj   +1 more source

Tracking mite trophic interactions by multiplex PCR

Pest Management Science, 2019
AbstractBACKGROUNDA thorough knowledge of trophic webs in agroecosystems is essential to achieve successful biological pest control. Phytoseiid mites are the most efficient natural enemies of tetranychid mites, which include several important pests worldwide.
Gómez-Martínez, María Antonia, Pina, Tatiana, Aguilar-Fenollosa, Ernestina, Jaques , Josep A., Hurtado-Ruiz, Monica   +4 more
openaire   +3 more sources