Results 61 to 70 of about 137,324 (248)

LINC01116, a hypoxia‐lncRNA marker of pathological lymphangiogenesis and poor prognosis in lung adenocarcinoma

Molecular Oncology, EarlyView.
The LINC01116 long noncoding RNA is induced by hypoxia and associated with poor prognosis and high recurrence rates in two cohorts of lung adenocarcinoma patients. Here, we demonstrate that besides its expression in cancer cells, LINC01116 is markedly expressed in lymphatic endothelial cells of the tumor stroma in which it participates in hypoxia ...
Marine Gautier‐Isola, Rafael Lopes Goncalves, Marin Truchi, Caroline Lacoux, Célia Scribe, Hugo Cadis, Laetitia Guardini, Sophie Bekisz, Marius Ilié, Paul Hofman, Georges Vassaux, Bernard Mari, Roger Rezzonico   +12 more
wiley   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Impact of Multiplex PCR on Diagnosis of Bacterial and Fungal Infections and Choice of Appropriate Antimicrobial Therapy

Diagnostics
Multiplex Polymerase Chain Reaction (PCR) has significantly impacted the field of infectious disease diagnostics, offering rapid and precise identification of bacterial and fungal pathogens.
Francesca Serapide, Rita Pallone, Angela Quirino, Nadia Marascio, Giorgio Settimo Barreca, Chiara Davoli, Rosaria Lionello, Giovanni Matera, Alessandro Russo   +8 more
doaj   +1 more source

Development of a GeXP-multiplex PCR assay for the simultaneous detection and differentiation of six cattle viruses. [PDF]

PLoS ONE, 2017
Foot-and-mouth disease virus (FMDV), Bluetongue virus (BTV), Vesicular stomatitis Virus (VSV), Bovine viral diarrheal (BVDV), Bovine rotavirus (BRV), and Bovine herpesvirus 1 (IBRV) are common cattle infectious viruses that cause a great economic loss ...
Qing Fan, Zhixun Xie, Zhiqin Xie, Xianwen Deng, Liji Xie, Li Huang, Sisi Luo, Jiaoling Huang, Yanfang Zhang, Tingting Zeng, Sheng Wang, Jiabo Liu, Yaoshan Pang   +12 more
doaj   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Development of Multiplex PCR for Simultaneous Detection of Trypanosoma evansi and Equine Piroplasma Infection in Horses

Hayati Journal of Biosciences
The identification of equine piroplasmosis and surra disease in infected horses currently presents a significant challenge. Clinical symptoms and blood smears are commonly used to diagnose these two diseases. The objective of this study was to develop a
Arifin Nugraha, Umi Cahyaningsih
doaj   +1 more source

Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping

BMC Genomics, 2008
Background Microsatellite (SSR) and single nucleotide polymorphism (SNP) markers are widely used in plant breeding and genomic research. Thus, methods to improve the speed and efficiency of SSR and SNP genotyping are highly desirable.
Nguyen Thao M, Hayden Matthew J, Waterman Amanda, Chalmers Kenneth J   +3 more
doaj   +1 more source

Multiplex PCR for rapid diagnosis of gastrointestinal tuberculosis

Journal of Global Infectious Diseases, 2013
Rapid and specific diagnosis of gastrointestinal tuberculosis (GITB) is of utmost importance.To evaluate Multiplex PCR (MPCR) using MPB64 and IS6110 primers specific for M. tuberculosis for rapid diagnosis of GITB.MPCR was performed on colonoscopy biopsy specimens on 11 GITB confirmed (culture/AFB/histopathology was positive), 29 GITB suspected and 30 ...
Kusum Sharma, Saroj Kant Sinha, Aman Sharma, Ritambra Nada, Kaushal K Prasad, Kapil Goyal, Surinder Singh Rana, Deepak Kumar Bhasin, Meera Sharma   +8 more
openaire   +3 more sources

Differential Diagnosis of Human Sparganosis Using Multiplex PCR [PDF]

The Korean Journal of Parasitology, 2018
Human sparganosis was diagnosed by morphological and genetic analyses in Korea. The complete mitochondrial genomes of Spirometra erinaceieuropaei and S. decipiens isolated in Korea have been recorded. Present study was performed to provide information to diagnose the etiologic agent of sparganosis by multiplex PCR using mitochondrial genome sequences ...
Jeon, Hyeong-Kyu, Kim, Kyu-Heon, Sohn, Woon-Mok, Eom, Keeseon S.   +3 more
openaire   +2 more sources

Monocyte LOXHD1 and RHOB Expression Predictive of Progressive Systemic Sclerosis–Associated Interstitial Lung Disease

Arthritis Care &Research, EarlyView.
Objective A leading cause of death among patients with scleroderma (SSc), interstitial lung disease (ILD) remains challenging to prognosticate. The discovery of biomarkers that accurately determine which patients would benefit from close monitoring and aggressive therapy would be an essential clinical tool.
Cristina M. Padilla, Robert Lafyatis, Kevin F. Gibson, Christina Morse, Eleanor Valenzi, Xiaoping Chen, Xiaoyun Li, Yanwu Zhao, Yongseok Park, Dana Ascherman, Jonathan Minden, Robyn Domsic, Yingze Zhang, Daniel J. Kass   +13 more
wiley   +1 more source