Results 51 to 60 of about 281,013 (338)

Development of multiplex real-time PCR assay for the detection of Brucella spp., Leptospira spp. and Campylobacter foetus [PDF]

Veterinaria Italiana, 2014
Abortion among dairy cattle is one of the major causes of economic losses in the livestock industry. This study describes a 1-step multiplex real-time polymerase chain reaction (PCR) to detect Brucella spp., Leptospira spp.
Abdelfattah M. Selim, Mahmoud M. Elhaig, Wolfgang Gaede   +2 more
doaj   +1 more source

Use of Whole Genome Phylogeny and Comparisons in the Development of a Multiplex-PCR Assay to Identify Sequence Type 36 Vibrio parahaemolyticus [PDF]

, 2015
Vibrio parahaemolyticus sequence type (ST) 36 strains that are native to the Pacific Ocean have recently caused multi-state outbreaks of gastroenteritis linked to shellfish harvested from the Atlantic Ocean.
Cooper, Vaughn S., Hall, Jeffrey A., Ilyas, Saba, Jones, Stephen H., Siwakoti, Puskar, Whistler, Cheryl A., Xu, Feng   +6 more
core   +1 more source

Multiplex PCR for avian pathogenic mycoplasmas

Molecular and Cellular Probes, 1997
Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production. A multiplex polymerase chain reaction (PCR) was optimized to simultaneously detect four pathogenic species of avian mycoplasmas. Four sets of oligonucleotide primers specific for Mycoplasma gallisepticum (MG), M.
H, Wang, A A, Fadl, M I, Khan
openaire   +2 more sources

Characterizing the salivary RNA landscape to identify potential diagnostic, prognostic, and follow‐up biomarkers for breast cancer

Molecular Oncology, EarlyView.
This study explores salivary RNA for breast cancer (BC) diagnosis, prognosis, and follow‐up. High‐throughput RNA sequencing identified distinct salivary RNA signatures, including novel transcripts, that differentiate BC from healthy controls, characterize histological and molecular subtypes, and indicate lymph node involvement.
Nicholas Rajan, Irina Primac, Emre Etlioglu, Laurens Debruyne, Ann Janssen, Magy Sallam, Kevin Tabury, Roel Quintens, Wiebren Tjalma, Mohammed Abderrafi Benotmane   +9 more
wiley   +1 more source

Comparison of Application of MD-VF-Auto SEM Method and Plankton Gene Multiplex PCR System in the Diagnosis of Drowning

Fayixue Zazhi, 2022
ObjectiveTo compare the application effect of microwave digestion - vacuum filtration - automated scanning electron microscopy （MD-VF-Auto SEM） method and plankton gene multiplex PCR system in the diagnosis of drowning.MethodsLung, liver and kidney ...
ZHANG Jian-miao, LIN Tian-chun, LIAO Zhen-yu, DU Yu-kun, YU Zhong-hao, LIU Jing-jian, WU Sai-qun, KANG Xiao-dong, XU Qu-yi, SHI He, ZHAO Jian, LIU Chao, GU Dang-en   +12 more
doaj   +1 more source

Mutation screening of the medium-chain acyl-CoA dehydrogenase (MCAD) and the ornithine transcarbamylase (OTC) genes by multiplex PCR amplification and sequencing [PDF]

, 2017
Background: Sequencing based mutation screening assays of genes encompassing large numbers of exons could be substantially optimized by multiplex PCR, which enables simultaneous amplification of many targets in one reaction.
Andrey, Gisela, Horn, Michael P., Largiadèr, Carlo R., Mäder-Heinemann, Gabriela   +3 more
core  

Bridging the gap: Multi‐stakeholder perspectives of molecular diagnostics in oncology

Molecular Oncology, EarlyView.
Although molecular diagnostics is transforming cancer care, implementing novel technologies remains challenging. This study identifies unmet needs and technology requirements through a two‐step stakeholder involvement. Liquid biopsies for monitoring applications and predictive biomarker testing emerge as key unmet needs. Technology requirements vary by
Jorine Arnouts, Senada Koljenović, Elise Daems, Karolien De Wael, Marc Peeters, Léon C. van Kempen, Greetje Vanhoutte, Karen Zwaenepoel, Timon Vandamme   +8 more
wiley   +1 more source

Successful gene expression analysis by multiplex, real-time, one-step RT-PCR, irrespective of the targets amplified

BioTechniques, 2007
Multiplex, real-time, one-step RT-PCR enables simultaneous quantification of several transcripts in one reaction vessel. This saves time and conserves precious sample material when analyzing the expression of reference and target genes in a particular ...
Holger Engel, Corinna Kueppers, Margaretha Koenig, Dirk Loeffert   +3 more
doaj   +1 more source

Development of novel DNA marker for species discrimination of Fasciola flukes based on the fatty acid binding protein type I gene

Parasites & Vectors, 2022
Background Multiplex polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) for nuclear phosphoenolpyruvate carboxykinase (pepck) and polymerase delta (pold), respectively, have been used to differentiate Fasciola ...
Emi Okamoto, Michiyo Tashiro, Pedro Ortiz, Uday Kumar Mohanta, Cristian Hobán, César A. Murga-Moreno, José M. Angulo-Tisoc, Madoka Ichikawa-Seki   +7 more
doaj   +1 more source

Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR [PDF]

, 2009
The increased occurrence of Salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. This study is aimed to detect the prevalence of twenty different virulence factors among Salmonella enterica strains ...
Awang Salleh, Noorzaleha, Cheah, Yoke Kqueen, Khoo, Chai Hoon, Lee, Learn Han, Mohd Sidik, Shiran, Radu, Son, Sim, Jiun Horng, Sukardi, Sabrina   +7 more
core   +1 more source