Results 81 to 90 of about 281,013 (338)

Microbiological, chemical and physical quality of drinking water for commercial turkeys: a cross-sectional study. [PDF]

, 2018
open9Drinking water for poultry is not subject to particular microbiological, chemical and physical requirements, thereby representing a potential transmission route for pathogenic microorganisms and contaminants and/or becoming unsuitable for water ...

core   +1 more source

LINC01116, a hypoxia‐lncRNA marker of pathological lymphangiogenesis and poor prognosis in lung adenocarcinoma

Molecular Oncology, EarlyView.
The LINC01116 long noncoding RNA is induced by hypoxia and associated with poor prognosis and high recurrence rates in two cohorts of lung adenocarcinoma patients. Here, we demonstrate that besides its expression in cancer cells, LINC01116 is markedly expressed in lymphatic endothelial cells of the tumor stroma in which it participates in hypoxia ...
Marine Gautier‐Isola, Rafael Lopes Goncalves, Marin Truchi, Caroline Lacoux, Célia Scribe, Hugo Cadis, Laetitia Guardini, Sophie Bekisz, Marius Ilié, Paul Hofman, Georges Vassaux, Bernard Mari, Roger Rezzonico   +12 more
wiley   +1 more source

Development of a GeXP-multiplex PCR assay for the simultaneous detection and differentiation of six cattle viruses. [PDF]

PLoS ONE, 2017
Foot-and-mouth disease virus (FMDV), Bluetongue virus (BTV), Vesicular stomatitis Virus (VSV), Bovine viral diarrheal (BVDV), Bovine rotavirus (BRV), and Bovine herpesvirus 1 (IBRV) are common cattle infectious viruses that cause a great economic loss ...
Qing Fan, Zhixun Xie, Zhiqin Xie, Xianwen Deng, Liji Xie, Li Huang, Sisi Luo, Jiaoling Huang, Yanfang Zhang, Tingting Zeng, Sheng Wang, Jiabo Liu, Yaoshan Pang   +12 more
doaj   +1 more source

Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt [PDF]

, 2019
Brucellosis is considered as endemic disease of animals and humans since thousands of years in Egypt. However, brucellosis in pigs has never been reported in Egypt.
El-Adawy, Hosny, El-Soally, Sherif Abdel Ghafar Elsayed, Elschner, Mandy C., Khan, Aman Ullah, Melzer, Falk, Mohamed, Shereen Aziz, Neubauer, Heinrich, Roesler, Uwe, Sayed Ahmed, Mohamed Abdelmonem   +8 more
core   +1 more source

MegaPlex PCR: a strategy for multiplex amplification [PDF]

Nature Methods, 2007
'MegaPlex PCR' is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair.
Meuzelaar, Linda Strömqvist, Lancaster, Owen, Pasche, J. Paul, Kopal, Guido, Brookes, Anthony J.   +4 more
openaire   +2 more sources

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Impact of Multiplex PCR on Diagnosis of Bacterial and Fungal Infections and Choice of Appropriate Antimicrobial Therapy

Diagnostics
Multiplex Polymerase Chain Reaction (PCR) has significantly impacted the field of infectious disease diagnostics, offering rapid and precise identification of bacterial and fungal pathogens.
Francesca Serapide, Rita Pallone, Angela Quirino, Nadia Marascio, Giorgio Settimo Barreca, Chiara Davoli, Rosaria Lionello, Giovanni Matera, Alessandro Russo   +8 more
doaj   +1 more source

Molecular analysis of PKU-associated PAH mutations: a fast and simple genotyping test [PDF]

, 2018
: Neonatal screening for phenylketonuria (PKU, OMIM: 261600) was introduced at the end of the 1960s. We developed a rapid and simple molecular test for the most frequent phenylalanine hydroxylase (PAH, Gene ID: 5053) mutations.
Antonio Angeloni, ARTIOLA, CRISTIANA, CARDUCCI, Carla, CARDUCCI, Claudia, D'AMICI, SIRIO, GIOVANNIELLO, TERESA, Manuela Tolve, PASQUALI, Amelia, PIZZUTI, Antonio, Vincenzo Leuzzi   +9 more
core   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Development of Multiplex PCR for Simultaneous Detection of Trypanosoma evansi and Equine Piroplasma Infection in Horses

Hayati Journal of Biosciences
The identification of equine piroplasmosis and surra disease in infected horses currently presents a significant challenge. Clinical symptoms and blood smears are commonly used to diagnose these two diseases. The objective of this study was to develop a
Arifin Nugraha, Umi Cahyaningsih
doaj   +1 more source