Results 121 to 130 of about 8,966 (157)

Detection and typing of lymphotropic herpesviruses by multiplex polymerase chain reaction

Journal of Virological Methods, 1999
A multiplex nested-polymerase chain reaction (PCR) method was developed for the simultaneous detection and typing of all human lymphotropic herpesviruses described to date, including Ebstein Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6, variants A and B (HHV6-A, HHV6-B), human herpesvirus 7 (HHV7) and human herpesvirus 8 (HHV8 ...
F, Pozo, A, Tenorio
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Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia*

Critical Care Medicine, 2008
To test a multiplex real-time polymerase chain reaction (PCR) method for simultaneous detection of multiple organisms in bloodstream infections.Prospective observational study at the University of California Davis Medical Center (Sacramento, CA). Two hundred adult (>18 yrs) patients from the emergency room, intensive care units, and general medicine ...
Richard F, Louie, Zuping, Tang, Timothy E, Albertson, Stuart, Cohen, Nam K, Tran, Gerald J, Kost   +5 more
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Multiplex Quantitative Fluorescent Polymerase Chain Reaction for Detection of Aneuploidies

Genetic Testing and Molecular Biomarkers, 2012
Quantitative fluorescent polymerase chain reaction (QF-PCR) is a well-established method in Western countries where commercial kits are being used for rapid prenatal diagnosis of aneuploidies. The limitations of these kits are that these are basically designed for the Caucasian population and are very expensive.
Shalu, Jain, Inusha, Panigrahi, Rekha, Gupta, Shubha R, Phadke, Sarita, Agarwal   +4 more
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Multiplex polymerase chain reaction.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 1992
The polymerase chain reaction (PCR) is a widely utilized assay for specifically amplifying small fragments of DNA. Multiplex PCR is the amplification of more than one DNA fragment per reaction and has many potential uses. When more than one primer set per reaction tube is utilized, the total number of tubes in any one experiment may be reduced ...
L J, Burgart, R A, Robinson, M J, Heller, W W, Wilke, O K, Iakoubova, J C, Cheville   +5 more
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Multiplex polymerase chain reaction of Alu polymorphic insertions

ELECTROPHORESIS, 1998
AbstractAlu sequences are present in humans in excess of 500 000 copies per haploid genome and represent the largest family of short interspersed repetitive elements (SINEs). These mobile genetic elements are ancestrally derived from the 7SL RNA gene and move throughout the genomes of primates by retroposition. Polymorphic Alu insertions have proven to
E, Thomas, R J, Herrera
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A Multiplex Polymerase Chain Reaction–Based Diagnostic Method for Bacterial Vaginosis

Obstetrics & Gynecology, 2002
To develop a polymerase chain reaction (PCR)-based diagnostic method for bacterial vaginosis using bacterial vaginosis-associated anaerobes.A multiple PCR assay was developed using primers specific to 16S ribosomal deoxyribonucleic acid (DNA) (Mobiluncus mulieris and Mobiluncus curtisii), nanH (Bacteroides fragilis), and an internal spacer region of ...
Mana, Obata-Yasuoka, William, Ba-Thein, Hiromi, Hamada, Hideo, Hayashi   +3 more
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Multiplex Polymerase Chain Reaction for the Diagnosis of Sexually Transmitted Diseases

Clinics in Laboratory Medicine, 1996
Sexually transmitted diseases (STD) are a major cause of death worldwide. Traditional laboratory methods of culture and serology are giving way to new DNA technologies, especially nucleic acid amplification. To date, polymerase chain reaction (PCR) has been the most widely used amplification method.
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Optimization of 3D Digital Microfluidic Biochips for the Multiplexed Polymerase Chain Reaction

ACM Transactions on Design Automation of Electronic Systems, 2016
A digital microfluidic biochip (DMFB) is an attractive technology platform for revolutionizing immunoassays, clinical diagnostics, drug discovery, DNA sequencing, and other laboratory procedures in biochemistry. In most of these applications, real-time polymerase chain reaction (PCR) is an indispensable step for amplifying specific DNA segments.
Zipeng Li, Tsung-Yi Ho, Krishnendu Chakrabarty   +2 more
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Human sex determination using multiplex polymerase chain reaction (PCR)

Science & Justice, 1996
A sex determination method has been developed using the polymerase chain reaction (PCR) which involved the amplification of the sex-determining region Y (SRY) gene and the amplification of the HLA-DQa gene as an internal control. This method, which can be applied to old and degraded DNA samples, allowed confident sexing of biological samples producing ...
Finch, Jennie Louise, Hope, Rory M., van Daal, A.   +2 more
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Classification of Giardia intestinalis isolates by multiple polymerase chain reaction (multiplex)

Parasitology Research, 2008
Agarose gel electrophoresis of gdh gene fragments, amplified by Multiplex, was used to classify the assemblage of 24 Giardia isolates obtained from axenic cultures, children's stools, and feces of puppies from different dog breeds. Isolates were compared with seven reference strains of Giardia intestinalis.
Leticia, Eligio-García, Adrián, Cortés-Campos, Enedina, Jiménez-Cardoso   +2 more
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