Journal of Clinical Laboratory Analysis, EarlyView.This assay enables multiplex genotyping of HLA‐CREGs and HPA alleles from whole blood using qPCR with an integrated parallel negative control. Results are interpreted based on amplification curves and Ct values, with validity dependent on strict quality control criteria for both the target reactions and the negative control.
Huimin Ji +9 more
wiley +1 more source
Multiplex Polymerase Chain Reaction Panels for Gastrointestinal Infections: Current Evidence, Regulatory Hurdles, and the Way Forward. [PDF]
Open Forum Infect DisTansarli GS, Allen DR, Fang FC.
europepmc +1 more source
Aging and periodontitis increase brain dissemination of oral bacteria
Journal of Periodontology, EarlyView.Abstract Background The microbiome is a dynamic system that changes throughout life. Studies have revealed the relationship between periodontal disease and the oral microbiota; however, the impact of periodontal disease on the expression of senescence markers and on the inflammaging of the oral and systemic microbiome remains unclear.
Ozge Unlu +7 more
wiley +1 more source
Real-world experience using multiplex polymerase chain reaction in intensive care unit patients with hospital-acquired and ventilator-associated pneumonia in South Korea. [PDF]
Acute Crit CareYoon EC +11 more
europepmc +1 more source
Journal of Surgical Oncology, EarlyView.ABSTRACT Sarcomas represent a diverse group of mesenchymal tumors with high rates of recurrence after resection. While recent technical advances have enabled the detection of rare circulating tumor DNA (ctDNA) in other malignancies, the complexity and heterogeneity of sarcoma genomics have historically limited ctDNA in these cancers.
Kristin E. Goodsell +5 more
wiley +1 more source
Multiplex Polymerase Chain Reaction (PCR) and Conventional Methods for Diagnosing Ventilator-Associated Pneumonia in ICU Settings: A Systematic Review. [PDF]
CureusFadel Yosif AS +5 more
europepmc +1 more source
Development of Multiplex Polymerase Chain Reaction (PCR)-Based MSA Assay for Bladder Cancer Detection. [PDF]
Int J Mol Sci, 2023 Reynolds T +4 more
europepmc +1 more source
Data‐Independent Acquisition Mass Spectrometry in Tumor Classification and Cancer Biomarker Research
Mass Spectrometry Reviews, EarlyView.Abstract Cancer treatment is far from optimal also because current classification systems do not reflect the complex molecular status of the tumor and its phenotype in sufficient detail. To construct molecular tumor classifiers, omics tools provide complex molecular data reflecting many aspects from genotype to phenotype.
Jan Simonik +3 more
wiley +1 more source
<i>Cubilin</i> gene mutation screening in Thai Border Collies using double-mismatch allele-specific and multiplex polymerase chain reaction: Implications for Imerslund-Gräsbeck syndrome. [PDF]
Vet WorldLerdkrai C +2 more
europepmc +1 more source
Medicine Bulletin, EarlyView.The figure was created in BioRender.com. ABSTRACT Accurate and early diagnosis remains a key challenge in modern disease management. Conventional diagnostic methods often suffer from limitations in sensitivity, spatial resolution, and practical usability.
Zizhuo Du +10 more
wiley +1 more source