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Diagnosing superinfection keratitis with multiplex polymerase chain reaction
Journal of Infection and Chemotherapy, 2018To report the potential usefulness of multiplex polymerase chain reaction (mPCR) for diagnosing superinfection keratitis caused by herpes simplex virus-1 (HSV-1), bacteria and fungus.Case series. Corneal scrapings were analyzed with mPCR for human herpes virus 1-8, bacterial 16S ribosomal DNA (rDNA) and fungal 28S rDNA.Case 1 was a 69-year-old man who ...
Sunao Sugita +2 more
exaly +3 more sources
Detection and typing of human herpesviruses by multiplex polymerase chain reaction
Journal of Virological Methods, 1993A new approach to simultaneous detection and typing of related agents by the multiplex polymerase chain reaction (PCR) is described. The reaction was been applied to human herpesviruses by nested amplification of fragments of the DNA polymerase genes.
Juan E Echevarría +2 more
exaly +3 more sources
Multiplexed digital polymerase chain reaction as a powerful diagnostic tool
Biosensors and Bioelectronics, 2021The digital polymerase chain reaction (dPCR) multiplexing method can simultaneously detect and quantify closely related deoxyribonucleic acid sequences in complex mixtures. The dPCR concept is continuously improved by the development of microfluidics and micro- and nanofabrication, and different complex techniques are introduced.
Martina Gaňová +4 more
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Reaction-Mapped Quantitative Multiplexed Polymerase Chain Reaction on a Microfluidic Device
Analytical Chemistry, 2008We have applied multiple-time-point reaction mapping to generate high-dynamic-range quantitative data from PCR multiplexes. The approach measures, then compensates, numerous PCR slope nonidealities across the multiplex without prejudice. A multilane microelectophoresis device with a novel scanning detector that reports redundantly over more than six ...
Jörn, Ueberfeld +4 more
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Multiplex gene removal by two-step polymerase chain reactions
Analytical Biochemistry, 2015Precise DNA manipulation is critical for molecular biotechnology. Restriction enzyme-based approaches are limited by their requirement of specific enzyme sites. Restriction-free cloning has greatly improved the flexibility and speed of precise DNA assembly. Most of these approaches focus on DNA assembly rather than gene removal.
Vishnu Vardhan, Krishnamurthy +4 more
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Multiplex polymerase chain reaction assay for genotyping Clostridium perfringens
American Journal of Veterinary Research, 1997Abstract Objective To develop a multiplex polymerase chain reaction (PCR) assay to detect the genes for the major toxins of Clostridium perfringens (cpa [α toxin], cpb [β toxin], etx [ε toxin], iA [ι i toxin], and cpe [enterotoxin]). Sample Population Cultures of C perfringens obtained from collections and diagnosticians throughout North America ...
R R, Meer, J G, Songer
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