Results 191 to 200 of about 3,481,864 (291)

Monocyte inflammation and adaptive immune dysfunction in severe Klebsiella pneumoniae pneumonia

iMeta, EarlyView.
Severe Klebsiella pneumoniae pneumonia combines intense inflammation with failure to control infection, yet the cellular basis is unclear. Using a severity‐focused single‐cell atlas of 708,894 peripheral blood mononuclear cells from 100 individuals, complemented by plasma protein measurements and flow cytometry, we show that disease severity redirects ...
Hongquan Chen, Xiaoling Wang, Xiang Liu, Kui Dong, Yanying Feng, Min Yuan, Shiyi Wang, Fangfang Fan, Yong Tian, Zhiming Kang, Xiushuai Du, Chaolu Hasi, Hong Lu, Wei Guan, Xu Chen, Mengyu Cheng, Wei Wei, Nan Guo, Zhiguo Liu, Xiaotong Qiu, Yiwei Shi, Kun Xiao, Yi Wang, Zhenjun Li   +23 more
wiley   +1 more source

Multiplex polymerase chain reaction (PCR) with Nanopore sequencing for sequence-based detection of four tilapia pathogens. [PDF]

PeerJ
Delamare-Deboutteville J, Meemetta W, Pimsannil K, Gan HM, Khor L, Chadag M, Dong HT, Senapin S.   +7 more
europepmc   +1 more source

Multiplex Real-time Polymerase Chain Reaction Assay [PDF]

, 2020
openaire   +1 more source

Dissecting the angiogenic mechanism of pachychoroid polypoidal choroidal vasculopathy

iMeta, EarlyView.
A prognosis‐oriented classification system was established to distinguish Pachy PCV from Non‐pachy PCV using a large multicenter dataset. Comparative plasma multi‐omics profiling of Pachy PCV, Non‐pachy PCV, and normal controls revealed unique upregulation of the fluid shear stress and atherosclerosis (FSS‐AS) and HIF‐1 signaling pathways in Pachy PCV.
Xinyu Zhao, Tiantian Cheng, Wenfei Zhang, Xingwang Gu, Qing Zhao, Yaning Chen, Lihui Meng, Jiaqi Zhang, Bintao Qiu, Lili Li, Shiyu Cheng, Zicheng Wang, Chuting Wang, Yuelin Wang, Zuyi Yang, Zhengming Shi, Ye Guo, Shengzhi Liu, Youxin Chen   +18 more
wiley   +1 more source

Rapid pathogen detection in synovial fluid of acute native joint infections in adults: a clinical evaluation of a novel automated multiplex polymerase chain reaction (mPCR) system. [PDF]

J Bone Jt Infect
Rabitsch L, Luger M, Lötsch F, Starzengruber P, Thalhammer F, Windhager R, Willinger B, Sigmund IK.   +7 more
europepmc   +1 more source

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

Correction to "Application of Nested Multiplex Polymerase Chain Reaction Respiratory and Pneumonia Panels in Children With Severe Community-Acquired Pneumonia". [PDF]

J Med Virol
europepmc   +1 more source

Impact of Respiratory Multiplex Polymerase Chain Reaction (PCR) on Antibiotic Stewardship: A Real-World Experience. [PDF]

Cureus
Bhattacharyya M, Todi S, Saha A.
europepmc   +1 more source

Adult Influenza Patients Diagnosed by Multiplex Polymerase Chain Reaction Tests, but Not Rapid Antigen Tests, and Managed Successfully During the 2024 to 2025 Major Influenza-Endemic Season in a Tertiary Hospital in Japan. [PDF]

Infect Drug Resist
Seki M.
europepmc   +1 more source

Low‐Concentration Enhancement Effect: Enabling Sensitive Detection of EML4‐ALK Variants via Multiplex RT‐MIRA

Journal of Clinical Laboratory Analysis, EarlyView.
This study developed an optimized multiplex RT‐MIRA assay for the simultaneous detection of EML4‐ALK variants, achieving single‐copy sensitivity. Unexpectedly, during the development of the reaction system, we discovered a “low‐concentration enhancement effect,” where reducing primer or probe concentrations within a specific range markedly improved ...
Xiao‐Dong Ren, Xi‐Yao Luo, Jin‐Mi Li, Xian‐Ge Sun, Jing Lv, Hua‐Liang Xiao, Qiu‐Shi Wang, Hong Wang, Chun‐Yan Wang, Hao‐Zheng Li, Qing Huang   +10 more
wiley   +1 more source