Results 251 to 260 of about 3,481,864 (291)

Detection and typing of lymphotropic herpesviruses by multiplex polymerase chain reaction

Journal of Virological Methods, 1999
A multiplex nested-polymerase chain reaction (PCR) method was developed for the simultaneous detection and typing of all human lymphotropic herpesviruses described to date, including Ebstein Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6, variants A and B (HHV6-A, HHV6-B), human herpesvirus 7 (HHV7) and human herpesvirus 8 (HHV8 ...
F, Pozo, A, Tenorio
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Comparison of DNA extraction methods for multiplex polymerase chain reaction

Analytical Biochemistry, 2010
We compared six DNA extraction methods for obtaining DNA from whole blood and saliva for use in multiplex polymerase chain reaction (PCR) assays. The aim was to evaluate saliva sampling as an alternative to blood sampling to obtain DNA for molecular diagnostics, genetic genealogy, and research purposes.
Triin, Viltrop, Kaarel, Krjutskov, Priit, Palta, Andres, Metspalu   +3 more
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Multiplex polymerase chain reaction.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 1992
The polymerase chain reaction (PCR) is a widely utilized assay for specifically amplifying small fragments of DNA. Multiplex PCR is the amplification of more than one DNA fragment per reaction and has many potential uses. When more than one primer set per reaction tube is utilized, the total number of tubes in any one experiment may be reduced ...
L J, Burgart, R A, Robinson, M J, Heller, W W, Wilke, O K, Iakoubova, J C, Cheville   +5 more
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Multiplex Quantitative Fluorescent Polymerase Chain Reaction for Detection of Aneuploidies

Genetic Testing and Molecular Biomarkers, 2012
Quantitative fluorescent polymerase chain reaction (QF-PCR) is a well-established method in Western countries where commercial kits are being used for rapid prenatal diagnosis of aneuploidies. The limitations of these kits are that these are basically designed for the Caucasian population and are very expensive.
Shalu, Jain, Inusha, Panigrahi, Rekha, Gupta, Shubha R, Phadke, Sarita, Agarwal   +4 more
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Multiplex polymerase chain reaction of Alu polymorphic insertions

ELECTROPHORESIS, 1998
AbstractAlu sequences are present in humans in excess of 500 000 copies per haploid genome and represent the largest family of short interspersed repetitive elements (SINEs). These mobile genetic elements are ancestrally derived from the 7SL RNA gene and move throughout the genomes of primates by retroposition. Polymorphic Alu insertions have proven to
E, Thomas, R J, Herrera
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Multiplex Polymerase Chain Reaction for the Diagnosis of Sexually Transmitted Diseases

Clinics in Laboratory Medicine, 1996
Sexually transmitted diseases (STD) are a major cause of death worldwide. Traditional laboratory methods of culture and serology are giving way to new DNA technologies, especially nucleic acid amplification. To date, polymerase chain reaction (PCR) has been the most widely used amplification method.
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A Multiplex Polymerase Chain Reaction–Based Diagnostic Method for Bacterial Vaginosis

Obstetrics & Gynecology, 2002
To develop a polymerase chain reaction (PCR)-based diagnostic method for bacterial vaginosis using bacterial vaginosis-associated anaerobes.A multiple PCR assay was developed using primers specific to 16S ribosomal deoxyribonucleic acid (DNA) (Mobiluncus mulieris and Mobiluncus curtisii), nanH (Bacteroides fragilis), and an internal spacer region of ...
Mana, Obata-Yasuoka, William, Ba-Thein, Hiromi, Hamada, Hideo, Hayashi   +3 more
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Optimization of 3D Digital Microfluidic Biochips for the Multiplexed Polymerase Chain Reaction

ACM Transactions on Design Automation of Electronic Systems, 2016
A digital microfluidic biochip (DMFB) is an attractive technology platform for revolutionizing immunoassays, clinical diagnostics, drug discovery, DNA sequencing, and other laboratory procedures in biochemistry. In most of these applications, real-time polymerase chain reaction (PCR) is an indispensable step for amplifying specific DNA segments.
Zipeng Li, Tsung-Yi Ho, Krishnendu Chakrabarty   +2 more
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Multiplex Polymerase Chain Reaction Diagnostics of Bed Bug (Hemiptera: Cimicidae)

Journal of Medical Entomology, 2011
Cimex lectularius L. (Hemiptera: Cimicidae) is a widespread blood feeding pest of humans around the world, including North America, and has recently undergone a resurgence. A molecular diagnostic technique applying multiplex polymerase chain reaction (PCR) was developed to distinguish bed bug eggs, leg fragments, and degraded samples from other ...
Allen L, Szalanski, Amber D, Tripodi, James W, Austin   +2 more
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Multiplex polymerase chain reaction for sex determination of single mouse blastomeres

Molecular Human Reproduction, 1995
Using a multiplex nested polymerase chain reaction (PCR) method with single copy genes and a dinucleotide repeat locus for the mouse Y and X chromosomes respectively, it was possible to discriminate between single cells derived from male and female embryos. Using single cells, amplification of Sry and Zfy sequences was not evident in all cases.
J C, Dreesen, J C, Dumoulin, J L, Evers, J P, Geraedts, M H, Pieters   +4 more
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